Synonym(s): 2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane

Description

In biological experiments, it is an important pH stabilizing reagent. Usually, an appropriate weak acid is mixed with its conjugate base to obtain a suitable pH value. Most biological reactions occur under neutral conditions, and the pH is generally selected between 6-8. Meanwhile, the effective buffering range of the buffer is required to be between 6-8 as well. In addition, it is required that the acidic and basic forms of the buffer do not undergo chelation with certain metal ions. It is also used for detecting creatine kinase, effectively protecting hemoglobin during freeze-drying; as a cathode electrolyte, it is applied in IEF/2-D gel electrophoresis.

Specifications

Item	Value
Chemical Name	Bis[2-Hydroxyethyl] imino Tris-(Hydroxymethyl)-methane
Appearance	White Crystal
CAS No.	6976-37-0
Molecular Formula	C8H19NO5
Molecular Weight	209.2
Grade	Biochemical Grade
Purity	≥99.0%
Melting Point	100.0-104.0℃
Loss on Drying	≤1.0%
Ignition Residue	≤0.1%
Water Solubility	Transparent
Heavy Metals	≤5ppm
Iron	≤5PPM
Chlorides	≤50PPM
pH (1M Aqueous Solution at 20℃)	9.0-11.0
PKa (25℃)	6.3-6.7
UV Absorbance (280nm, 1mol/L, 1cm)	≤0.40
Storage / Handling	Store at Room Temperature.

Features

High Purity (≥99%) ensuring reliable performance in sensitive biochemical and molecular workflows.
Zwitterionic, stable buffering capacity near pH 6.0–7.0, ideal for maintaining physiological conditions.
Low UV absorbance suitable for spectrophotometric and fluorescence-based assays.
Excellent water solubility for easy preparation of buffer solutions and stock formulations.
Low impurity profile with minimal heavy metals, chloride, and ignition residue for consistent experimental results.

Applications

Protein purification and biochemical assays requiring stable and low-interference buffering conditions.
Electrophoresis systems, including running, gel, and sample buffers for protein and nucleic acid separation.
Cell culture and bioprocessing buffers to help maintain pH stability during cell growth or expression studies.
Analytical and preparative chromatography, ensuring reproducible separation performance.
Diagnostic reagent formulations where high purity and low background absorbance are essential.

Safety Disclaimer

This product is intended for laboratory research use only. Not for drug, human, or veterinary use. Review the Safety Data Sheet (SDS) provided by BioFargo for detailed handling, storage, hazard, and disposal information. Use appropriate personal protective equipment (PPE) including gloves, lab coat, and eye protection when handling. Dispose of waste and residual buffer in accordance with local, state, and federal regulations. Ensure proper ventilation if preparing large volumes or heating solutions.

Documents

SDS

Bis-Tris 99% Biochemical Grade