{"product_id":"biofargo-nhei-gmp-grade-restriction-enzyme-20-u-µl","title":"Biofargo NheI, GMP Grade Restriction Enzyme (20 U\/µL)","description":"\u003cdiv style=\"width: 100%; margin: 0; padding: 0; line-height: 1.7; color: #222222;\"\u003e\n\u003ch2 style=\"margin-top: 0; padding-top: 0;\"\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eBiofargo NheI, GMP Grade is a recombinant restriction endonuclease expressed in \u003cem\u003eEscherichia coli\u003c\/em\u003e and supplied at 20 U\/µL with its matching 10× CutOne® Buffer, GMP Grade. It recognizes the sequence G^CTAGC and completely digests target DNA in 15 minutes to 1 hour at 37 °C.\u003c\/p\u003e\n\u003cp\u003eThe enzyme is manufactured and quality-controlled in compliance with GMP specifications, with full traceability of the production process and raw materials. No antibiotics and no animal-derived raw materials or excipients are used anywhere in production. Process-related impurities are tightly controlled, including host cell protein, host cell DNA, non-specific endonuclease, DNase and RNase activity, microbial limits and bacterial endotoxin. According to the manufacturer, the product meets the requirements for raw materials in fields such as vaccine and pharmaceutical production.\u003c\/p\u003e\n\u003cp\u003eEach lot is tested for host cell DNA, host cell protein, microbial limit, bacterial endotoxin and heavy metals using methods from the Chinese Pharmacopoeia (ChP 2025, Volume IV), and for mycoplasma using a LAMP-based detection kit.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eGMP Grade:\u003c\/strong\u003e “GMP Grade” describes the manufacturing and quality-control standard of this reagent. It is not a claim of suitability for clinical use; users are responsible for qualifying the material for their own processes.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResearch Use Only:\u003c\/strong\u003e This product is intended for laboratory research use only. It is not intended for diagnostic, therapeutic, food, household, or clinical use.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cdiv style=\"width: 100%; overflow-x: auto; -webkit-overflow-scrolling: touch;\"\u003e\n\u003ctable style=\"width: 100%; min-width: 560px; border-collapse: collapse; border: 1px solid #dddddd;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; padding: 12px 15px; border: 1px solid #dddddd; background-color: #f7f7f7; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 12px 15px; border: 1px solid #dddddd; vertical-align: middle;\"\u003eNheI, GMP Grade\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; background-color: #f7f7f7; vertical-align: middle;\"\u003e\u003cstrong\u003eCatalog Number\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; vertical-align: middle;\"\u003eGMP505S\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; background-color: #f7f7f7; vertical-align: middle;\"\u003e\u003cstrong\u003ePackage Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; vertical-align: middle;\"\u003e1 mL enzyme + 6 × 1 mL 10× CutOne® Buffer, GMP Grade\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; background-color: #f7f7f7; vertical-align: middle;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; vertical-align: middle;\"\u003e20 U\/µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; background-color: #f7f7f7; vertical-align: middle;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; vertical-align: middle;\"\u003e5'...G^CTAGC...3'\u003cbr\u003e3'...CGATC^G...5'\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; background-color: #f7f7f7; vertical-align: middle;\"\u003e\u003cstrong\u003eIsoschizomers\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; vertical-align: middle;\"\u003eAsuNHI (isoschizomer); BmtI, BspOI (neoschizomers, which produce different ends)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; background-color: #f7f7f7; vertical-align: middle;\"\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; vertical-align: middle;\"\u003eRecombinant, expressed in \u003cem\u003eE. coli\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; background-color: #f7f7f7; vertical-align: middle;\"\u003e\u003cstrong\u003eSupplied Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; vertical-align: middle;\"\u003e10× CutOne® Buffer, GMP Grade\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; background-color: #f7f7f7; vertical-align: middle;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; vertical-align: middle;\"\u003eOne unit is the amount of enzyme required to completely digest 1 µg of λDNA (HindIII digest) in 1 hour at 37 °C in a 50 µL reaction.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; background-color: #f7f7f7; vertical-align: middle;\"\u003e\u003cstrong\u003eOptimal Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; vertical-align: middle;\"\u003e37 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; background-color: #f7f7f7; vertical-align: middle;\"\u003e\u003cstrong\u003eHeat Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; vertical-align: middle;\"\u003e80 °C for 20 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; background-color: #f7f7f7; vertical-align: middle;\"\u003e\u003cstrong\u003eMethylation Sensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; vertical-align: middle;\"\u003eCleavage may be impaired by overlapping CpG methylation.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; background-color: #f7f7f7; vertical-align: middle;\"\u003e\u003cstrong\u003eProtein Purity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; vertical-align: middle;\"\u003e≥95% (SDS-PAGE, Coomassie Blue staining)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; background-color: #f7f7f7; vertical-align: middle;\"\u003e\u003cstrong\u003eAnimal-Derived Components\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; vertical-align: middle;\"\u003eNone (animal-component-free)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; background-color: #f7f7f7; vertical-align: middle;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; vertical-align: middle;\"\u003e−20 ± 5 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; background-color: #f7f7f7; vertical-align: middle;\"\u003e\u003cstrong\u003eShipping Condition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; vertical-align: middle;\"\u003eShipped at ≤0 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; background-color: #f7f7f7; vertical-align: middle;\"\u003e\u003cstrong\u003eShelf Life\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; vertical-align: middle;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; background-color: #f7f7f7; vertical-align: middle;\"\u003e\u003cstrong\u003eIntended Use\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; vertical-align: middle;\"\u003eResearch use only; GMP-grade product\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch2\u003eQuality Control\u003c\/h2\u003e\n\u003cdiv style=\"width: 100%; overflow-x: auto; -webkit-overflow-scrolling: touch;\"\u003e\n\u003ctable style=\"width: 100%; min-width: 560px; border-collapse: collapse; border: 1px solid #dddddd;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; padding: 12px 15px; border: 1px solid #dddddd; background-color: #f7f7f7; vertical-align: middle;\"\u003e\u003cstrong\u003eProtein Purity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 12px 15px; border: 1px solid #dddddd; vertical-align: middle;\"\u003e≥95% as determined by SDS-PAGE with Coomassie Blue staining\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; background-color: #f7f7f7; vertical-align: middle;\"\u003e\u003cstrong\u003eStar Activity \/ Prolonged Incubation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; vertical-align: middle;\"\u003eNo detectable degradation by other nucleases or star activity after 16 hours at 37 °C with 20 U and 1 µg λDNA (HindIII digest) in 50 µL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; background-color: #f7f7f7; vertical-align: middle;\"\u003e\u003cstrong\u003eNon-Specific Endonuclease Activity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; vertical-align: middle;\"\u003e\u0026lt;20% conversion of 1 µg supercoiled plasmid to the nicked or linearized form after 4 hours at 37 °C with 20 U (50 µL reaction).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; background-color: #f7f7f7; vertical-align: middle;\"\u003e\u003cstrong\u003eDNase Activity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; vertical-align: middle;\"\u003eNo detectable degradation of 15 ng dsDNA fragments after 16 hours at 37 °C with 20 U (20 µL reaction).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; background-color: #f7f7f7; vertical-align: middle;\"\u003e\u003cstrong\u003eRNase Activity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; vertical-align: middle;\"\u003e\u0026gt;90% of 500 ng RNA remains intact after 1 hour at 37 °C with 20 U (10 µL reaction).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; background-color: #f7f7f7; vertical-align: middle;\"\u003e\u003cstrong\u003eHost Cell DNA\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; vertical-align: middle;\"\u003e\u0026lt;10 copies\/20 U of residual \u003cem\u003eE. coli\u003c\/em\u003e DNA (qPCR, ChP 2025 Vol. IV, General Chapter 3407, Method 3)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; background-color: #f7f7f7; vertical-align: middle;\"\u003e\u003cstrong\u003eHost Cell Protein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; vertical-align: middle;\"\u003e\u0026lt;50 ppm residual \u003cem\u003eE. coli\u003c\/em\u003e host cell protein (ChP 2025 Vol. IV, General Chapter 3412)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; background-color: #f7f7f7; vertical-align: middle;\"\u003e\u003cstrong\u003eMicrobial Limit\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; vertical-align: middle;\"\u003eTotal aerobic microbial count ≤5 cfu\/mL; total combined yeasts\/molds count ≤5 cfu\/mL (ChP 2025 Vol. IV, General Chapter 1105)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; background-color: #f7f7f7; vertical-align: middle;\"\u003e\u003cstrong\u003eBacterial Endotoxin\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; vertical-align: middle;\"\u003e\u0026lt;0.5 EU\/KU (gel-clot, ChP 2025 Vol. IV, General Chapter 1143, Method 1)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; background-color: #f7f7f7; vertical-align: middle;\"\u003e\u003cstrong\u003eMycoplasma\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; vertical-align: middle;\"\u003eNegative (20 U tested, LAMP method)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; background-color: #f7f7f7; vertical-align: middle;\"\u003e\u003cstrong\u003eHeavy Metals\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 15px; border: 1px solid #dddddd; vertical-align: middle;\"\u003e\u0026lt;10 ppm (ChP 2025 Vol. IV, General Chapter 0821, Method 1)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch2\u003eKey Features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGMP-compliant manufacturing:\u003c\/strong\u003e Produced and quality-controlled under GMP specifications with full traceability of the process and raw materials.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAnimal-component-free and antibiotic-free:\u003c\/strong\u003e No animal-derived materials, excipients or antibiotics are used in production.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFast, complete digestion:\u003c\/strong\u003e Digests target DNA in 15 minutes to 1 hour at 37 °C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eComprehensive impurity control:\u003c\/strong\u003e Each lot is tested for host cell DNA, host cell protein, endotoxin, microbial limit, mycoplasma and heavy metals.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh purity:\u003c\/strong\u003e ≥95% pure by SDS-PAGE, with no detectable DNase or non-specific nuclease contamination under the specified test conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMatched GMP-grade buffer:\u003c\/strong\u003e Supplied with 6 × 1 mL of 10× CutOne® Buffer, GMP Grade.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHeat inactivation:\u003c\/strong\u003e Inactivated by incubation at 80 °C for 20 minutes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRestriction cloning and subcloning\u003c\/li\u003e\n\u003cli\u003ePlasmid linearization for in vitro transcription (IVT) templates\u003c\/li\u003e\n\u003cli\u003eConstruction of expression vectors for vaccine and biopharmaceutical development\u003c\/li\u003e\n\u003cli\u003eRestriction mapping and DNA fragment preparation\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eInstructions for Use\u003c\/h2\u003e\n\u003cp\u003eCombine the following components on ice in the order shown:\u003c\/p\u003e\n\u003cdiv style=\"width: 100%; overflow-x: auto; -webkit-overflow-scrolling: touch;\"\u003e\n\u003ctable style=\"width: 100%; min-width: 560px; border-collapse: collapse; border: 1px solid #dddddd;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px 12px; border: 1px solid #dddddd; background-color: #f7f7f7; text-align: center;\"\u003e\u003cstrong\u003eReagent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 12px; border: 1px solid #dddddd; background-color: #f7f7f7; text-align: center;\"\u003e\u003cstrong\u003eVolume\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px 12px; border: 1px solid #dddddd; text-align: center;\"\u003eddH\u003csub\u003e2\u003c\/sub\u003eO\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 12px; border: 1px solid #dddddd; text-align: center;\"\u003eUp to 50 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px 12px; border: 1px solid #dddddd; text-align: center;\"\u003e10× CutOne® Buffer, GMP Grade\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 12px; border: 1px solid #dddddd; text-align: center;\"\u003e5 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px 12px; border: 1px solid #dddddd; text-align: center;\"\u003eDNA\u003csup\u003ea\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 12px; border: 1px solid #dddddd; text-align: center;\"\u003e1 µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px 12px; border: 1px solid #dddddd; text-align: center;\"\u003eNheI, GMP Grade (20 U\/µL)\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 12px; border: 1px solid #dddddd; text-align: center;\"\u003e1 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px 12px; border: 1px solid #dddddd; text-align: center;\"\u003e\u003cstrong\u003eTotal\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 12px; border: 1px solid #dddddd; text-align: center;\"\u003e\u003cstrong\u003e50 µL\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003csup\u003ea\u003c\/sup\u003e DNA substrates should be free of phenol, chloroform, ethanol, EDTA, detergents and high salt concentrations, which can reduce enzyme activity.\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eMix gently by pipetting or flicking the tube (do not vortex), then spin down briefly.\u003c\/li\u003e\n\u003cli\u003eIncubate at 37 °C for 15 minutes to 1 hour.\u003c\/li\u003e\n\u003cli\u003eInactivate the enzyme by heating at 80 °C for 20 minutes.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003cstrong\u003eNotes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eThe enzyme volume should not exceed 10% of the total reaction volume, to avoid star activity caused by excess glycerol from the enzyme storage buffer.\u003c\/li\u003e\n\u003cli\u003eAdditives in the enzyme storage buffer (e.g. glycerol, salt) and contaminants in the DNA solution (e.g. salt, EDTA, ethanol) inhibit digestion more strongly in smaller reaction volumes.\u003c\/li\u003e\n\u003cli\u003eStar activity does not occur under the recommended conditions. Extended incubation or high enzyme concentrations may result in star activity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eNumber of Recognition Sites in Common DNAs\u003c\/h3\u003e\n\u003cdiv style=\"width: 100%; overflow-x: auto; -webkit-overflow-scrolling: touch;\"\u003e\n\u003ctable style=\"width: 100%; min-width: 560px; border-collapse: collapse; border: 1px solid #dddddd;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px 12px; border: 1px solid #dddddd; background-color: #f7f7f7; text-align: center;\"\u003e\u003cstrong\u003eλDNA\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 12px; border: 1px solid #dddddd; background-color: #f7f7f7; text-align: center;\"\u003e\u003cstrong\u003eΦX174\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 12px; border: 1px solid #dddddd; background-color: #f7f7f7; text-align: center;\"\u003e\u003cstrong\u003epBR322\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 12px; border: 1px solid #dddddd; background-color: #f7f7f7; text-align: center;\"\u003e\u003cstrong\u003epUC57\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 12px; border: 1px solid #dddddd; background-color: #f7f7f7; text-align: center;\"\u003e\u003cstrong\u003epUC18\/19\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 12px; border: 1px solid #dddddd; background-color: #f7f7f7; text-align: center;\"\u003e\u003cstrong\u003eSV40\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 12px; border: 1px solid #dddddd; background-color: #f7f7f7; text-align: center;\"\u003e\u003cstrong\u003eM13mp18\/19\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 12px; border: 1px solid #dddddd; background-color: #f7f7f7; text-align: center;\"\u003e\u003cstrong\u003eAdeno2\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px 12px; border: 1px solid #dddddd; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 12px; border: 1px solid #dddddd; text-align: center;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 12px; border: 1px solid #dddddd; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 12px; border: 1px solid #dddddd; text-align: center;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 12px; border: 1px solid #dddddd; text-align: center;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 12px; border: 1px solid #dddddd; text-align: center;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 12px; border: 1px solid #dddddd; text-align: center;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 12px; border: 1px solid #dddddd; text-align: center;\"\u003e4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3\u003eMethylation Effects on Digestion\u003c\/h3\u003e\n\u003cdiv style=\"width: 100%; overflow-x: auto; -webkit-overflow-scrolling: touch;\"\u003e\n\u003ctable style=\"width: 100%; min-width: 560px; border-collapse: collapse; border: 1px solid #dddddd;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px 12px; border: 1px solid #dddddd; background-color: #f7f7f7; text-align: center;\"\u003e\u003cstrong\u003eDam\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 12px; border: 1px solid #dddddd; background-color: #f7f7f7; text-align: center;\"\u003e\u003cstrong\u003eDcm\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 12px; border: 1px solid #dddddd; background-color: #f7f7f7; text-align: center;\"\u003e\u003cstrong\u003eCpG\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 12px; border: 1px solid #dddddd; background-color: #f7f7f7; text-align: center;\"\u003e\u003cstrong\u003eEcoKI\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 12px; border: 1px solid #dddddd; background-color: #f7f7f7; text-align: center;\"\u003e\u003cstrong\u003eEcoBI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px 12px; border: 1px solid #dddddd; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 12px; border: 1px solid #dddddd; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 12px; border: 1px solid #dddddd; text-align: center;\"\u003eImpaired\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 12px; border: 1px solid #dddddd; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 12px; border: 1px solid #dddddd; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch2\u003eStorage and Handling\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eStore at −20 ± 5 °C.\u003c\/li\u003e\n\u003cli\u003eShelf life is 24 months when stored under the recommended conditions.\u003c\/li\u003e\n\u003cli\u003eShipped at ≤0 °C.\u003c\/li\u003e\n\u003cli\u003eKeep the enzyme on ice during reaction setup and return it to −20 °C promptly after use.\u003c\/li\u003e\n\u003cli\u003eUse appropriate laboratory protective equipment when handling.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Biofargo_GMP505S_NheI_GMP_Grade_Manual.pdf?v=1793720042\" rel=\"noopener noreferrer\" type=\"application\/pdf\" target=\"_blank\"\u003e NheI, GMP Grade – Instruction Manual (GMP505S) \u003c\/a\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe Safety Data Sheet (SDS) and lot-specific Certificate of Analysis (COA) are available on request. Please \u003ca href=\"\/pages\/contact-us\"\u003econtact us\u003c\/a\u003e with the catalog number and lot number.\u003c\/p\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cdetails open=\"\"\u003e\n\u003csummary\u003e\u003cstrong\u003eWhat makes NheI, GMP Grade different from a standard NheI?\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cp\u003eNheI, GMP Grade is manufactured and quality-controlled under GMP specifications, with full raw-material traceability, no animal-derived components and no antibiotics. Each lot is additionally tested for host cell DNA, host cell protein, endotoxin, microbial limit, mycoplasma and heavy metals.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eWhat is the recognition sequence of NheI?\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cp\u003eNheI recognizes G^CTAGC. AsuNHI is an isoschizomer; BmtI and BspOI are neoschizomers that recognize the same sequence but produce different ends. Isoschizomers and neoschizomers may have different methylation sensitivities.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eWhich buffer should I use?\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cp\u003eUse the supplied 10× CutOne® Buffer, GMP Grade at 1× final concentration (5 µL in a 50 µL reaction).\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eHow long should I incubate the reaction?\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cp\u003eIncubate at 37 °C for 15 minutes to 1 hour. Longer incubation or excess enzyme may lead to star activity.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eCan NheI be heat-inactivated?\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cp\u003eYes. Heat at 80 °C for 20 minutes to inactivate the enzyme.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eIs NheI sensitive to DNA methylation?\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cp\u003eCleavage may be impaired by overlapping CpG methylation.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eHow should the enzyme be stored?\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cp\u003eStore at −20 ± 5 °C. The shelf life is 24 months under the recommended conditions. The product is shipped at ≤0 °C.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eIs this product approved for clinical use?\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cp\u003eNo. “GMP Grade” describes the manufacturing and quality-control standard. The product is labeled for research use only, and users must qualify it for their own manufacturing processes.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdiv id=\"display-search-keyword\" style=\"display: none;\"\u003enhei gmp, gmp grade nhei, nhei restriction enzyme, gmp restriction endonuclease, plasmid linearization enzyme, animal-free nhei\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Biofargo","offers":[{"title":"1 mL","offer_id":53663550111925,"sku":"BFE4005","price":1500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Biofargo_GMP505S_NheI_GMP_Grade.jpg?v=1793720026","url":"https:\/\/biofargo.com\/products\/biofargo-nhei-gmp-grade-restriction-enzyme-20-u-%c2%b5l","provider":"Biofargo","version":"1.0","type":"link"}