Description

Human Epithelial Cell Medium Kit is a low-serum medium system for primary epithelial cells from a wide range of tissues, including the respiratory and digestive tracts, cornea, kidney, mammary gland and prostate. It promotes attachment and spreading and maintains viability, proliferation, epithelial phenotype and key functions.

Each kit combines 480 mL of epithelial basal medium with one of two 20 mL supplements: EpiGM-M for most epithelial cell types, or EpiGM-B for retinal pigment epithelium (RPE). The supplements contain recombinant human EGF and transferrin and a small amount (2% v/v) of premium FBS to reduce senescence of primary epithelial cells.

Specifications

Parameter	Specification
Product Name	Human Epithelial Cell Medium Kit
Catalog Numbers	BFEPIGMM500 (EpiGM-M) / BFEPIGMB500 (EpiGM-B, RPE)
Format	Basal medium + supplement
Volume	480 mL basal medium + 20 mL supplement
Serum	2% (v/v) FBS
Key Supplements	Recombinant human EGF, transferrin
pH	7.2–7.4
Endotoxin	≤ 0.25 EU/mL
Filtration	0.2 µm
Sterility	Bacteria and fungi not detected
Antibiotics	None
Shelf Life	12 months
Intended Use	For research use only

Features

Two matched supplements: EpiGM-M for general epithelia, EpiGM-B for retinal pigment epithelium.
Recombinant human EGF and transferrin for epithelial proliferation.
Low serum (2%) to reduce senescence of primary epithelial cells.
Supports attachment, spreading and epithelial phenotype.
Low endotoxin (≤ 0.25 EU/mL); no antibiotics or antifungals.

Applications

EpiGM-M: bronchial and alveolar, mammary, corneal and conjunctival, renal and proximal tubule, prostate epithelial cells and sebocytes.
EpiGM-B: retinal pigment epithelial (RPE) cells.
Barrier & Toxicology Studies: Epithelial monolayer and permeability assays.
Disease Modeling: Primary epithelial cultures for respiratory, renal and ocular research.

Kit Components

Component	Volume	Storage
Epithelial Basal Medium	480 mL	4 °C
EpiGM-M or EpiGM-B Supplement	20 mL	−20 °C

Storage & Shipping

Store the basal medium at 4 °C and the supplement at −20 °C. Shelf life 12 months.
Complete medium: store at 2–8 °C and use within 4 weeks.
Shipped with ice packs and dry ice.

Protocol Overview

Thaw the supplement at 4 °C overnight or for 1–2 hours at room temperature, then add it to the basal medium (or mix 4 mL supplement with 96 mL basal medium for smaller batches).
Add 0.5–1% antibiotics if desired.
Seed primary epithelial cells at 1–1.5 × 10⁴ cells/cm² and change the medium every other day until 70–80% confluent.
For routine passaging, seed at 5–8 × 10³ cells/cm².

Frequently Asked Questions (FAQs)

Common questions about Human Epithelial Cell Medium Kit.

Which supplement should I choose?

Choose EpiGM-B for retinal pigment epithelial (RPE) cells and EpiGM-M for other epithelial cells, such as airway, mammary, corneal, renal and prostate epithelium.

Is the medium serum-free?

No. It contains a low level (2% v/v) of premium FBS to reduce senescence of primary epithelial cells.

Does it contain antibiotics?

No. Antibiotics (0.5–1%) can be added if desired.

How long is the complete medium stable?

Store at 2–8 °C and use within 4 weeks.

Can it be used for clinical or diagnostic purposes?

No. This product is for research use only and is not intended for human, diagnostic or therapeutic use.

Documents

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