Name: hES/iPSC Suspension Culture Medium Kit (AOF)
SKU: BFIPSCS500

Can this medium support organoid research?

Yes, suspension cultured aggregates can transition into 3D differentiation workflows.

Description

Scaling human pluripotent stem cell (hPSC) culture from routine research workflows to larger expansion systems remains challenging when using conventional adherent culture methods. Limited surface area, dependence on extracellular matrix coating, manual passaging steps, and difficulties maintaining consistent cell aggregates can affect expansion efficiency and downstream applications.

hES/iPSC Suspension Culture Medium Kit (AOF) is an animal-origin-free (AOF) human pluripotent stem cell culture medium designed for feeder-free suspension expansion of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). This iPSC suspension culture medium enables the formation of uniform cell aggregates, supporting scalable 3D stem cell culture workflows without the requirement for microcarriers.

Designed for researchers developing scalable hPSC expansion workflows, hES/iPSC Suspension Culture Medium Kit supports suspension-based iPSC expansion, spheroid formation, and downstream differentiation applications including organoid research. The system provides a flexible solution for transitioning between suspension (3D) and adherent (2D) culture formats.

Specifications

Product Name	hES/iPSC Suspension Culture Medium Kit (AOF)
SKU	BFIPSCS500
Product Type	Human pluripotent stem cell suspension culture medium
Application Format	3D suspension culture of hESC and iPSC
Composition	Animal-origin-free (AOF)
Basal Medium	496 mL
Additive	4 mL
Filtration	<0.2 μm
Endotoxin	≤0.125 EU/mL
Bacteria Detection	Not detected
Fungi Detection	Not detected
Storage	Basal medium: 2–8°C Additive: -20°C
Shelf Life	12 months

Features

Feeder-free iPSC suspension culture medium designed for human pluripotent stem cell expansion.
Animal-origin-free formulation suitable for defined stem cell culture workflows.
Supports microcarrier-free 3D suspension culture of hESC and iPSC.
Promotes formation of relatively uniform cell aggregates mainly distributed around 200–300 μm.
Enables transition between suspension culture and adherent culture workflows.
Supports scalable hPSC expansion from multi-well plates toward bioreactor-based culture systems.
Designed for downstream 3D differentiation and organoid research applications.
Filtered using a <0.2 μm filtration process for consistent culture preparation.

Performance Data

Suspension Expansion of Human iPSC

hES/iPSC Suspension Culture Medium Kit was evaluated using human iPSC suspension culture workflows. In ultra-low attachment 6-well plates, iPSC aggregates were expanded under orbital shaking conditions, demonstrating efficient suspension-based cell expansion.

Human iPSC suspension expansion performance using hES/iPSC suspension culture medium in ultra-low attachment plates

In a disposable bioreactor-based suspension culture workflow, hES/iPSC Suspension Culture Medium supported scalable expansion conditions suitable for larger-volume hPSC culture evaluation.

Bioreactor scale-up suspension culture of human iPSC using stem cell expansion medium

Uniform iPSC Aggregate Formation

Suspension culture with medium enables formation of three-dimensional human pluripotent stem cell aggregates. Uniform aggregate size distribution can help support consistent downstream differentiation workflows.

Uniform iPSC spheroid aggregate formation during 3D suspension stem cell culture

Maintenance of Pluripotent Characteristics

Long-term suspension culture evaluation demonstrated maintenance of commonly used pluripotency markers including SSEA-3 and TRA-1-81 in human iPSC and embryonic stem cell cultures.

SSEA-3 and TRA-1-81 pluripotency marker analysis of hPSC suspension culture

Applications

HES/iPSC Suspension Culture Medium Kit is designed for researchers requiring scalable and reproducible human pluripotent stem cell expansion solutions. The suspension culture format supports applications where traditional adherent workflows may become limiting.

Human induced pluripotent stem cell (iPSC) suspension expansion
Human embryonic stem cell (hESC) 3D suspension culture
Feeder-free pluripotent stem cell culture workflows
Large-scale hPSC expansion research
3D stem cell aggregate and spheroid culture
Organoid differentiation research using iPSC-derived cells
Stem cell bioprocess development studies
Bioreactor-compatible suspension culture research
Human pluripotent stem cell characterization studies
Regenerative medicine and disease modeling research

Why Choose hES/iPSC Suspension Culture Medium?

Researchers working with human pluripotent stem cells increasingly require culture systems that support scalability, consistency, and compatibility with advanced 3D biology workflows. Suspension-based hPSC expansion provides an alternative approach when conventional adherent culture methods become difficult to scale.

hES/iPSC Suspension Culture Medium Kit combines feeder-free suspension expansion, animal-origin-free formulation, and 3D aggregate culture capability to support modern iPSC research workflows including organoid development and scalable stem cell culture studies.

FAQ

What is hES/iPSC Suspension Culture Medium Kit used for?

This product is a human pluripotent stem cell suspension culture medium designed for expansion of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) in 3D suspension workflows.

What is the advantage of iPSC suspension culture compared with adherent culture?

Suspension culture can reduce dependence on surface coating materials and provide a scalable approach for generating human pluripotent stem cell aggregates.

Does this medium support feeder-free iPSC culture?

Yes. hES/iPSC Suspension Culture Medium Kit is designed for feeder-free hPSC expansion workflows.

What size cell aggregates are generated during suspension culture?

The product supports formation of relatively uniform cell aggregates, mainly distributed around 200–300 μm according to evaluation data.

Can hES/iPSC Suspension Medium be used for organoid research?

Yes. Suspension-cultured hPSC aggregates can be directly transitioned into 3D differentiation workflows including organoid research applications.

Is this an animal-origin-free stem cell culture medium?

Yes. hES/iPSC Suspension Culture Medium Kit is designed as an animal-origin-free (AOF) culture system.

Can this medium support bioreactor-based hPSC expansion?

The product has been evaluated in disposable bioreactor suspension culture workflows, supporting scalable hPSC expansion research.

How should hES/iPSC Suspension Culture Medium be stored?

The basal medium should be stored at 2–8°C and the additive should be stored at -20°C.

hESC/iPSC Suspension Expansion Medium Kit (AOF)