Name: Biofargo hES/iPSC Adherent Culture Medium Kit (High-protein, AOF)
Brand: Biofargo
SKU: BFIPSCA500

What does AOF mean in stem cell culture medium?

AOF means animal-origin-free. The formulation is designed without animal-origin components.

Can Biofargo hES/iPSC Medium support feeder-free iPSC culture?

Yes. Biofargo hES/iPSC Medium is designed for feeder-free human ES and iPSC adherent culture workflows.

Description

Maintaining reliable expansion of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) requires a culture environment that supports cell proliferation while preserving key pluripotency characteristics. During routine stem cell culture workflows, variations in cell recovery after thawing, passage adaptation, and expansion efficiency may affect the availability of high-quality pluripotent cell populations for downstream research.

Biofargo hES/iPSC Adherent Culture Medium Kit (High-protein, AOF) is a human pluripotent stem cell culture medium designed for feeder-free adherent culture of human ES cells and iPS cells. The formulation contains recombinant human serum albumin and provides a defined culture environment without animal-origin components or human-origin components, supporting consistent stem cell maintenance and expansion workflows.

This AOF iPSC culture medium is developed for researchers requiring reliable human pluripotent stem cell expansion while maintaining commonly evaluated pluripotency markers. Performance evaluation demonstrated enhanced cell expansion compared with TeSR™-AOF under tested conditions, while maintaining comparable cell viability and pluripotency marker expression including SSEA-3 and TRA-1-81.

Specifications

Product Name	Biofargo hES/iPSC Adherent Culture Medium Kit (High-protein, AOF)
Product Type	Human pluripotent stem cell culture medium
Product SKU	BFIPSCA500
Culture Application	Adherent culture of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs)
Formulation	Animal-origin-free (AOF) formulation containing recombinant human serum albumin
Basal Medium	496 mL
Additive	4 mL
Filtration	<0.2 μm
Endotoxin	Basal medium: ≤0.25 EU/mL Additive: ≤0.125 EU/mL
Bacteria Detection	Not detected
Fungi Detection	Not detected
Storage Condition	Basal medium: 2–8°C Additive: -20°C
Shelf Life	12 months
Production Standard	Research grade / GMP grade

Features

Animal-origin-free (AOF) formulation designed for human pluripotent stem cell culture workflows without animal-origin components.
High-protein culture formulation containing recombinant human serum albumin to support iPSC proliferation and culture performance.
Designed for feeder-free iPSC culture and adherent maintenance of human ES and iPS cells.
Supports human pluripotent stem cell expansion with enhanced cell recovery and proliferation performance.
Compatible with evaluation of commonly used pluripotency markers including SSEA-3 and TRA-1-81.
Provides a defined culture system for researchers performing hESC and iPSC maintenance studies.
Manufactured with filtration and quality control testing including endotoxin, bacteria, and fungi detection.

Performance Data

Enhanced iPSC Expansion Performance Compared with TeSR™-AOF

Biofargo hES/iPSC High-protein AOF Medium was evaluated against TeSR™-AOF using continuous five-passage human iPSC culture experiments. High-passage iPSC cells were recovered and expanded using adherent culture conditions on Corning Matrigel-coated plates. Cell expansion performance was analyzed by AO/PI staining-based viable cell counting.

Human iPSC expansion comparison between Biofargo high protein AOF medium and TeSR AOF culture medium

The evaluation showed that Biofargo high-protein AOF medium produced higher cumulative viable cell numbers compared with TeSR™-AOF under tested culture conditions.

Expansion Performance Across Different iPSC Sources

Three different human iPSC cell sources were evaluated using Biofargo high-protein AOF medium and TeSR™-AOF. Average expansion performance across five passages was analyzed to assess consistency among different iPSC backgrounds.

Expansion performance analysis of multiple human iPSC lines cultured with Biofargo hES iPSC medium

Biofargo hES/iPSC Medium demonstrated higher average expansion performance across tested iPSC sources while maintaining comparable culture characteristics.

Cell Viability Evaluation During iPSC Expansion

Human iPSC viability analysis during expansion using animal origin free stem cell culture medium

AO/PI staining analysis showed comparable cell viability between Biofargo high-protein AOF medium and TeSR™-AOF during the evaluation period.

Pluripotency Marker Maintenance

Maintaining pluripotency characteristics is an important consideration during human pluripotent stem cell culture. Biofargo hES/iPSC Medium was evaluated using SSEA-3 and TRA-1-81 expression analysis.

SSEA 3 pluripotency marker expression analysis of human iPSC cultured with Biofargo AOF medium

SSEA-3 expression levels showed no significant difference between Biofargo high-protein AOF medium and TeSR™-AOF under tested conditions.

TRA 1 81 pluripotency marker expression analysis of human iPSC culture medium comparison

TRA-1-81 expression analysis demonstrated comparable pluripotency marker maintenance between tested culture conditions.

Applications

Biofargo hES/iPSC Adherent Culture Medium Kit (High-protein, AOF) is designed for researchers performing human pluripotent stem cell culture workflows that require consistent maintenance and expansion of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). The animal-origin-free formulation and high-protein culture environment support routine feeder-free adherent stem cell culture applications.

Human induced pluripotent stem cell (iPSC) expansion
Supports expansion workflows where researchers require increased recovery and proliferation performance during repeated iPSC culture passages.
Human embryonic stem cell (hESC) culture
Suitable for adherent culture maintenance of human embryonic stem cells under defined culture conditions.
Feeder-free iPSC culture workflows
Designed for feeder-free human pluripotent stem cell culture systems requiring reliable maintenance and expansion.
Human pluripotent stem cell maintenance
Supports routine hPSC culture workflows while maintaining commonly evaluated pluripotency characteristics.
High-passage iPSC expansion studies
Suitable for researchers evaluating expansion performance after multiple culture passages.
iPSC recovery after thawing
Provides a culture environment for recovery and subsequent expansion of thawed human iPSC populations.
Stem cell characterization studies
Compatible with workflows evaluating pluripotency markers including SSEA-3 and TRA-1-81.
Human iPSC disease modeling research
Supports preparation and expansion of human iPSC populations used in stem cell research models.
Stem cell differentiation research preparation
Provides expanded pluripotent stem cell populations for downstream differentiation research workflows.
Basic human pluripotent stem cell biology research
Suitable for laboratory studies requiring consistent hESC/iPSC culture conditions.

Why Choose Biofargo hES/iPSC Culture Medium?

Human pluripotent stem cell culture requires a reliable environment that supports cell expansion while preserving important characteristics associated with pluripotency. Differences in cell source, passage number, and recovery conditions may influence expansion performance during routine hESC and iPSC culture workflows.

Biofargo hES/iPSC Adherent Culture Medium Kit combines an animal-origin-free (AOF) formulation with recombinant human serum albumin to provide a defined culture solution for feeder-free human pluripotent stem cell expansion. The formulation is designed to support researchers looking for a consistent iPSC culture medium for adherent hESC and iPSC maintenance.

In comparative evaluation against TeSR™-AOF, Biofargo high-protein AOF medium demonstrated increased cumulative viable cell numbers during five-passage iPSC expansion experiments while maintaining comparable cell viability and pluripotency marker expression.

FAQ

What is Biofargo hES/iPSC Adherent Culture Medium Kit used for?

Biofargo hES/iPSC Adherent Culture Medium Kit is a human pluripotent stem cell culture medium designed for feeder-free maintenance and expansion of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs).

Is Biofargo hES/iPSC Medium suitable for iPSC expansion?

Yes. Biofargo hES/iPSC Medium is designed as an iPSC expansion medium for adherent culture workflows. Performance evaluation demonstrated enhanced cumulative viable cell expansion compared with TeSR™-AOF under tested conditions.

What does AOF mean in iPSC culture medium?

AOF means animal-origin-free. Biofargo hES/iPSC Adherent Culture Medium Kit uses an animal-origin-free formulation without animal-origin components or human-origin components, providing a defined option for human pluripotent stem cell culture workflows.

Can this medium be used for feeder-free iPSC culture?

Yes. Biofargo hES/iPSC Adherent Culture Medium Kit is designed for feeder-free human ES cell and iPSC adherent culture workflows.

What is the advantage of the high-protein formulation?

The high-protein formulation contains recombinant human serum albumin, which provides additional protein support within the culture system and contributes to enhanced iPSC proliferation performance observed during evaluation.

Which pluripotency markers were evaluated with Biofargo hES/iPSC Medium?

Biofargo hES/iPSC Medium was evaluated using commonly used human pluripotent stem cell markers including SSEA-3 and TRA-1-81. Both markers showed comparable expression levels compared with TeSR™-AOF under tested conditions.

How was Biofargo iPSC culture medium evaluated for expansion performance?

Expansion performance was evaluated through continuous five-passage human iPSC culture experiments. Viable cell numbers were analyzed using AO/PI staining-based cell counting methods.

What cells are compatible with Biofargo hES/iPSC Culture Medium?

Biofargo hES/iPSC Culture Medium is intended for human embryonic stem cells (hESCs) and human induced pluripotent stem cells (iPSCs) maintained under adherent culture conditions.

How should Biofargo hES/iPSC Medium be stored?

The basal medium should be stored at 2–8°C, while the additive component should be stored at -20°C.

hESC/iPSC Adherent Expansion Medium Kit (High-Protein, AOF)