{"product_id":"biofargo-bspqi-gmp-grade","title":"Biofargo BspQI, GMP Grade","description":"\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eBiofargo BspQI, GMP Grade is a restriction endonuclease produced by recombinant expression in \u003cem\u003eE. coli\u003c\/em\u003e and manufactured under GMP specifications, for use as a raw material in mRNA, plasmid and vaccine production. BspQI (an isoschizomer of SapI) is a Type IIS enzyme recognising GCTCTTC and cutting one base downstream, outside the site. Its seven-base recognition sequence is rarer than a six-base site, and it is unaffected by Dam, Dcm and CpG methylation, so it linearises cleanly whatever host the plasmid was grown in. It runs at 50 °C.\u003c\/p\u003e\n\u003cp\u003eManufacturing is fully traceable through the process and raw materials. No antibiotics, animal-derived materials or animal-derived excipients are used anywhere in the process. Process-related impurities are controlled and released against specification: host cell protein, residual host DNA, non-specific endonuclease, DNase and RNase activity, microbial limits, bacterial endotoxin, mycoplasma and heavy metals.\u003c\/p\u003e\n\u003cp\u003eThe enzyme digests target DNA in 15 minutes to 1 hour at 50 °C and is heat-inactivated at 80 °C for 20 minutes. It is supplied at 10 U\/µL with six vials of the matching 10× Cut Buffer C, GMP Grade, so no buffer has to be ordered separately. A Certificate of Analysis is issued for every lot.\u003c\/p\u003e\n\u003cp\u003eFor Research Use Only unless qualified by the customer for its intended manufacturing use. Not for use in diagnostic or therapeutic procedures.\u003c\/p\u003e\n\n\u003c\/div\u003e\n\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 90%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eParameter\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eSpecification\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eProduct Name\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eBiofargo BspQI, GMP Grade\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eCatalog Number\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eBF-E4003\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eGrade\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eGMP Grade\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eEnzyme Type\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eType IIS restriction endonuclease\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eRecognition \/ Cut Site\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e5′…G C T C T T C(N)\u003csub\u003e1\u003c\/sub\u003e…3′\u003cbr\u003e3′…C G A G A A G(N)\u003csub\u003e4\u003c\/sub\u003e…5′\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eIsoschizomers\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eSapI, LguI, PciSI (methylation sensitivities may differ)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eConcentration\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e10 U\/µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003ePack Size\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e10,000 U (1 mL)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eSupplied Buffer\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e10× Cut Buffer C, GMP Grade, 6 × 1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eReaction Temperature\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e50 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eDigestion Time\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e15 minutes to 1 hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eHeat Inactivation\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e80 °C for 20 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eUnit Definition\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eCompletely digest 1 µg of λDNA in 1 hour at 50 °C in a 50 µL reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eProtein Purity\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e≥95% by SDS-PAGE (Coomassie Blue)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eHost Cell Protein\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u0026lt;50 ppm (ChP 2025 Vol. IV, General Chapter 3412)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eResidual Host DNA\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u0026lt;10 copies\/10 U (ChP 2025 Vol. IV, General Chapter 3407, qPCR)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eBacterial Endotoxin\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u0026lt;10 EU\/mg (ChP 2025 Vol. IV, General Chapter 1143, gel-clot)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eMicrobial Limit\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eTotal aerobic count \u0026lt;5 cfu\/mL; yeasts and moulds \u0026lt;5 cfu\/mL (ChP 2025 Vol. IV, General Chapter 1105)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eMycoplasma\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eNegative (LAMP)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eHeavy Metals\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u0026lt;10 ppm (ChP 2025 Vol. IV, General Chapter 0821)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eAnimal-Derived Components\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eNone\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eAntibiotics\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eNone used in manufacture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e−20 ± 5 °C, valid 24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eShipping\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e≤0 °C (blue ice)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eDocumentation\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eLot-specific Certificate of Analysis\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eGMP manufacture with full traceability\u003c\/strong\u003e of process and raw materials, for use as a raw material in mRNA, plasmid and vaccine production.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eType IIS, scarless\u003c\/strong\u003e — cleavage occurs outside the recognition sequence, so no residual bases are left at the cut and the 3′ end of a linearised template is defined by the construct rather than by the enzyme.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eNo animal-derived components and no antibiotics\u003c\/strong\u003e anywhere in the process, which removes two of the questions a raw-material compliance review always asks.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eImpurities released against specification\u003c\/strong\u003e — host cell protein, residual host DNA, non-specific endonuclease, DNase, RNase, microbial limits, endotoxin, mycoplasma and heavy metals, each by a stated pharmacopoeial method.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eBuffer included\u003c\/strong\u003e — six 1 mL vials of 10× Cut Buffer C, GMP Grade ship with the enzyme, so the digest does not depend on a separate buffer order.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eFast, complete digestion\u003c\/strong\u003e in 15 minutes to 1 hour, with heat inactivation at 80 °C for 20 minutes.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eLot-specific Certificate of Analysis\u003c\/strong\u003e with every shipment.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003e24-month shelf life\u003c\/strong\u003e at −20 ± 5 °C.\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003ePlasmid linearisation for IVT:\u003c\/strong\u003e Linearise the DNA template before in vitro transcription, where template integrity and a defined 3′ end set the transcript length and translation efficiency.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eGolden Gate and modular assembly:\u003c\/strong\u003e Cut and ligate in one reaction with no scar between parts.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003emRNA manufacture:\u003c\/strong\u003e Supply the upstream digestion step as a GMP-grade raw material with documented impurity control.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003ePlasmid DNA production:\u003c\/strong\u003e Linearise and verify plasmid constructs under a controlled process.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eVaccine and cell therapy manufacture:\u003c\/strong\u003e Use as a raw material where animal-component-free and antibiotic-free sourcing has to be demonstrated.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eProcess development:\u003c\/strong\u003e Run the same enzyme at research scale and at manufacturing scale without changing supplier or revalidating.\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 90%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eVolume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eBspQI, GMP Grade (10 U\/µL)\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e10,000 U (1 mL)\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e−20 ± 5 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e10× Cut Buffer C, GMP Grade\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e6 × 1 mL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e−20 ± 5 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cp\u003eShip at ≤0 °C on blue ice.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eStore at −20 ± 5 °C. Valid for 24 months.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eAvoid repeated freeze-thaw cycles; keep the enzyme on ice while setting up reactions.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eA lot-specific Certificate of Analysis is supplied with every shipment.\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eProtocol Overview\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003e\u003cp\u003eCombine on ice, in order: nuclease-free water to 50 µL, 5 µL of 10× Cut Buffer C, GMP Grade, 1 µg of DNA, and 1 µL of BspQI, GMP Grade (10 U\/µL).\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eMix gently and spin down.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eIncubate at 50 °C for 15 minutes to 1 hour.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eInactivate by heating at 80 °C for 20 minutes.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eKeep the enzyme below 10% of the total reaction volume to avoid star activity from carried-over glycerol.\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/div\u003e\n\u003cstyle\u003e\n  .bf-rich .josh-faq { background: #fff; color: #000; font-family: \"IBM Plex Sans\", sans-serif; }\n  .bf-rich .josh-faq__inner { padding: 24px 0 40px; max-width: 100%; box-sizing: border-box; }\n  .bf-rich .josh-faq__item { border-bottom: 1px solid #e5e7eb; padding: 0; }\n  .bf-rich .josh-faq__item:first-child { border-top: 1px solid #e5e7eb; }\n  .bf-rich .josh-faq__question { width: 100%; margin: 0; padding: 20px 0; display: flex; align-items: center; justify-content: space-between; gap: 16px; color: #505052; background: none; border: 0; font: 700 16px\/1.4 \"IBM Plex Sans\", sans-serif; text-align: left; cursor: pointer; list-style: none; }\n  .bf-rich .josh-faq__question::-webkit-details-marker { display: none; }\n  .bf-rich .josh-faq__question:hover { color: #505052; }\n  .bf-rich .josh-faq__icon { position: relative; width: 20px; height: 20px; flex: 0 0 20px; transition: transform .25s ease; }\n  .bf-rich .josh-faq__icon::before, .bf-rich .josh-faq__icon::after { position: absolute; content: \"\"; background: currentColor; border-radius: 2px; transition: opacity .25s ease, transform .25s ease; }\n  .bf-rich .josh-faq__icon::before { top: 50%; left: 0; width: 100%; height: 2px; transform: translateY(-50%); }\n  .bf-rich .josh-faq__icon::after { top: 0; left: 50%; width: 2px; height: 100%; transform: translateX(-50%); }\n  .bf-rich .josh-faq__item[open] .josh-faq__icon::after { opacity: 0; transform: translateX(-50%) rotate(90deg); }\n  .bf-rich .josh-faq__answer { max-width: 860px; padding: 0 0 20px; color: #505052; font: 400 16px\/1.6 \"IBM Plex Sans\", sans-serif; }\n  .bf-rich .josh-faq__answer p { margin: 0 0 15px; color: #505052; font: inherit; }\n  .bf-rich .josh-faq__answer p:last-child { margin-bottom: 0; }\n  .bf-rich .josh-faq__answer ul { margin: 8px 0 12px; padding-left: 22px; }\n  .bf-rich .josh-faq__answer li { margin-bottom: 6px; font: inherit; list-style-type: disc; }\n  @media (max-width: 767px) { .bf-rich .josh-faq__inner { padding: 24px 0 40px; } .bf-rich .josh-faq__question { padding: 16px 0; font-size: 15px; } .bf-rich .josh-faq__answer { font-size: 15px; } }\n\u003c\/style\u003e\n\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eFrequently Asked Questions (FAQs)\u003c\/h2\u003e\n\u003cp\u003eCommon questions about Biofargo BspQI, GMP Grade.\u003c\/p\u003e\n\u003cdiv class=\"josh-faq\"\u003e\u003csection class=\"josh-faq__inner\"\u003e\u003cdiv class=\"josh-faq__list\"\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\u003csummary class=\"josh-faq__question\"\u003eWhat does GMP Grade mean for this enzyme?\u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\u003cdiv class=\"josh-faq__answer\"\u003e\u003cp\u003eIt is manufactured and released under GMP specifications, with full traceability of the process and of the raw materials, and with process-related impurities controlled to a written specification. Host cell protein, residual host DNA, non-specific endonuclease, DNase and RNase activity, microbial limits, bacterial endotoxin, mycoplasma and heavy metals are each tested by a stated pharmacopoeial method and reported on the lot Certificate of Analysis.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\u003csummary class=\"josh-faq__question\"\u003eAre animal-derived materials or antibiotics used?\u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\u003cdiv class=\"josh-faq__answer\"\u003e\u003cp\u003eNo. Neither animal-derived materials or excipients nor antibiotics are used anywhere in the manufacturing process. This is normally the first question in a raw-material compliance review, so it is stated on the Certificate of Analysis.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\u003csummary class=\"josh-faq__question\"\u003eWhat impurity levels are specified?\u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\u003cdiv class=\"josh-faq__answer\"\u003e\u003cp\u003eProtein purity is ≥95% by SDS-PAGE. Host cell protein is \u0026lt;50 ppm, residual host DNA \u0026lt;10 copies\/10 U, bacterial endotoxin \u0026lt;10 EU\/mg, heavy metals \u0026lt;10 ppm, total aerobic microbial count \u0026lt;5 cfu\/mL with yeasts and moulds \u0026lt;5 cfu\/mL, and mycoplasma negative by LAMP.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\u003csummary class=\"josh-faq__question\"\u003eHow is a digest set up?\u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\u003cdiv class=\"josh-faq__answer\"\u003e\u003cp\u003eOn ice, combine 5 µL of 10× Cut Buffer C, GMP Grade, 1 µg of DNA, 1 µL of enzyme and nuclease-free water to 50 µL. Mix gently, spin down, incubate at 50 °C for 15 minutes to 1 hour, then inactivate at 80 °C for 20 minutes.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\u003csummary class=\"josh-faq__question\"\u003eWhy should the enzyme stay below 10% of the reaction volume?\u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\u003cdiv class=\"josh-faq__answer\"\u003e\u003cp\u003eThe enzyme storage buffer contains glycerol. Above about 10% of the total reaction volume, the carried-over glycerol can cause star activity. The same logic applies in reverse to contaminants in the DNA: the smaller the reaction, the more strongly salt, EDTA or ethanol in the substrate inhibits digestion.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\u003csummary class=\"josh-faq__question\"\u003eWhat can interfere with the digest?\u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\u003cdiv class=\"josh-faq__answer\"\u003e\u003cp\u003eDNA substrates should carry no phenol, chloroform, ethanol, EDTA, detergent or high salt. All of these reduce enzyme activity, and their effect is proportionally worse in a small reaction volume.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\u003csummary class=\"josh-faq__question\"\u003eIs cleavage affected by methylation?\u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\u003cdiv class=\"josh-faq__answer\"\u003e\u003cp\u003eIt is not — Dam, Dcm, CpG, EcoKI and EcoBI methylation all leave cleavage unaffected, so the template can come from any common host.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\u003csummary class=\"josh-faq__question\"\u003eDoes it come with buffer?\u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\u003cdiv class=\"josh-faq__answer\"\u003e\u003cp\u003eYes. Six 1 mL vials of 10× Cut Buffer C, GMP Grade ship with every unit, so the digest does not depend on ordering a buffer separately.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\u003csummary class=\"josh-faq__question\"\u003eHow is it stored and shipped?\u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\u003cdiv class=\"josh-faq__answer\"\u003e\u003cp\u003eIt ships at ≤0 °C on blue ice and is stored at −20 ± 5 °C, where it is valid for 24 months.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\u003csummary class=\"josh-faq__question\"\u003eCan you support a DMF filing or a custom GMP enzyme?\u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\u003cdiv class=\"josh-faq__answer\"\u003e\u003cp\u003eYes. Drug Master File support and custom GMP enzyme manufacture can be arranged. Contact us at contact@biofargo.com with the enzyme, the scale and the regulatory route you need.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\u003csummary class=\"josh-faq__question\"\u003eDo you provide a Certificate of Analysis?\u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\u003cdiv class=\"josh-faq__answer\"\u003e\u003cp\u003eYes. A lot-specific Certificate of Analysis is issued with every shipment and can be sent in advance for a raw-material qualification review. Request it at contact@biofargo.com.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\u003csummary class=\"josh-faq__question\"\u003eCan it be used for clinical or diagnostic purposes?\u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\u003cdiv class=\"josh-faq__answer\"\u003e\u003cp\u003eNo. This product is for research use only and is not intended for human, diagnostic or therapeutic use.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\n\u003c\/div\u003e\u003c\/section\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cscript type=\"application\/ld+json\"\u003e{\"@context\": \"https:\/\/schema.org\", \"@type\": \"FAQPage\", \"mainEntity\": [{\"@type\": \"Question\", \"name\": \"What does GMP Grade mean for this enzyme?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"It is manufactured and released under GMP specifications, with full traceability of the process and of the raw materials, and with process-related impurities controlled to a written specification. Host cell protein, residual host DNA, non-specific endonuclease, DNase and RNase activity, microbial limits, bacterial endotoxin, mycoplasma and heavy metals are each tested by a stated pharmacopoeial method and reported on the lot Certificate of Analysis.\"}}, {\"@type\": \"Question\", \"name\": \"Are animal-derived materials or antibiotics used?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"No. Neither animal-derived materials or excipients nor antibiotics are used anywhere in the manufacturing process. This is normally the first question in a raw-material compliance review, so it is stated on the Certificate of Analysis.\"}}, {\"@type\": \"Question\", \"name\": \"What impurity levels are specified?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"Protein purity is \u0026ge;95% by SDS-PAGE. Host cell protein is \u0026lt;50 ppm, residual host DNA \u0026lt;10 copies\/10 U, bacterial endotoxin \u0026lt;10 EU\/mg, heavy metals \u0026lt;10 ppm, total aerobic microbial count \u0026lt;5 cfu\/mL with yeasts and moulds \u0026lt;5 cfu\/mL, and mycoplasma negative by LAMP.\"}}, {\"@type\": \"Question\", \"name\": \"How is a digest set up?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"On ice, combine 5 \u0026#181;L of 10\u0026times; Cut Buffer C, GMP Grade, 1 \u0026#181;g of DNA, 1 \u0026#181;L of enzyme and nuclease-free water to 50 \u0026#181;L. Mix gently, spin down, incubate at 50 \u0026#176;C for 15 minutes to 1 hour, then inactivate at 80 \u0026#176;C for 20 minutes.\"}}, {\"@type\": \"Question\", \"name\": \"Why should the enzyme stay below 10% of the reaction volume?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"The enzyme storage buffer contains glycerol. Above about 10% of the total reaction volume, the carried-over glycerol can cause star activity. The same logic applies in reverse to contaminants in the DNA: the smaller the reaction, the more strongly salt, EDTA or ethanol in the substrate inhibits digestion.\"}}, {\"@type\": \"Question\", \"name\": \"What can interfere with the digest?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"DNA substrates should carry no phenol, chloroform, ethanol, EDTA, detergent or high salt. All of these reduce enzyme activity, and their effect is proportionally worse in a small reaction volume.\"}}, {\"@type\": \"Question\", \"name\": \"Is cleavage affected by methylation?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"It is not \u0026mdash; Dam, Dcm, CpG, EcoKI and EcoBI methylation all leave cleavage unaffected, so the template can come from any common host.\"}}, {\"@type\": \"Question\", \"name\": \"Does it come with buffer?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"Yes. Six 1 mL vials of 10\u0026times; Cut Buffer C, GMP Grade ship with every unit, so the digest does not depend on ordering a buffer separately.\"}}, {\"@type\": \"Question\", \"name\": \"How is it stored and shipped?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"It ships at \u0026le;0 \u0026#176;C on blue ice and is stored at \u0026minus;20 \u0026plusmn; 5 \u0026#176;C, where it is valid for 24 months.\"}}, {\"@type\": \"Question\", \"name\": \"Can you support a DMF filing or a custom GMP enzyme?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"Yes. Drug Master File support and custom GMP enzyme manufacture can be arranged. Contact us at contact@biofargo.com with the enzyme, the scale and the regulatory route you need.\"}}, {\"@type\": \"Question\", \"name\": \"Do you provide a Certificate of Analysis?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"Yes. A lot-specific Certificate of Analysis is issued with every shipment and can be sent in advance for a raw-material qualification review. Request it at contact@biofargo.com.\"}}, {\"@type\": \"Question\", \"name\": \"Can it be used for clinical or diagnostic purposes?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"No. This product is for research use only and is not intended for human, diagnostic or therapeutic use.\"}}]}\u003c\/script\u003e\n\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eMethylation Effects on Digestion\u003c\/h2\u003e\n\u003ctable style=\"width:100%;border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding:6px 10px;text-align:center;\"\u003eDam\u003c\/td\u003e\n\u003ctd style=\"padding:6px 10px;text-align:center;\"\u003eDcm\u003c\/td\u003e\n\u003ctd style=\"padding:6px 10px;text-align:center;\"\u003eCpG\u003c\/td\u003e\n\u003ctd style=\"padding:6px 10px;text-align:center;\"\u003eEcoKI\u003c\/td\u003e\n\u003ctd style=\"padding:6px 10px;text-align:center;\"\u003eEcoBI\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding:6px 10px;text-align:center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"padding:6px 10px;text-align:center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"padding:6px 10px;text-align:center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"padding:6px 10px;text-align:center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"padding:6px 10px;text-align:center;\"\u003eNo effect\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2\u003eNumber of Recognition Sites in Common DNA\u003c\/h2\u003e\n\u003ctable style=\"width:100%;border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding:6px 8px;text-align:center;\"\u003eλDNA\u003c\/td\u003e\n\u003ctd style=\"padding:6px 8px;text-align:center;\"\u003eΦX174\u003c\/td\u003e\n\u003ctd style=\"padding:6px 8px;text-align:center;\"\u003epBR322\u003c\/td\u003e\n\u003ctd style=\"padding:6px 8px;text-align:center;\"\u003epUC57\u003c\/td\u003e\n\u003ctd style=\"padding:6px 8px;text-align:center;\"\u003epUC18\/19\u003c\/td\u003e\n\u003ctd style=\"padding:6px 8px;text-align:center;\"\u003eSV40\u003c\/td\u003e\n\u003ctd style=\"padding:6px 8px;text-align:center;\"\u003eM13mp18\/19\u003c\/td\u003e\n\u003ctd style=\"padding:6px 8px;text-align:center;\"\u003eAdeno2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding:6px 8px;text-align:center;\"\u003e10\u003c\/td\u003e\n\u003ctd style=\"padding:6px 8px;text-align:center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"padding:6px 8px;text-align:center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"padding:6px 8px;text-align:center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"padding:6px 8px;text-align:center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"padding:6px 8px;text-align:center;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"padding:6px 8px;text-align:center;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"padding:6px 8px;text-align:center;\"\u003e7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cp\u003eThe Biofargo GMP-grade restriction endonuclease range covers both scarless Type IIS sites and the conventional backbone sites, so a change of plasmid backbone does not mean a change of supplier or a second raw-material qualification.\u003c\/p\u003e\n\u003cul\u003e\n  \u003cli\u003e\u003cp\u003e\u003ca href=\"\/products\/biofargo-bsai-gmp-grade\"\u003e\u003cstrong\u003eBiofargo BsaI, GMP Grade\u003c\/strong\u003e\u003c\/a\u003e — Type IIS, scarless cleavage; 20 U\/µL, 20,000 U.\u003c\/p\u003e\u003c\/li\u003e\n  \u003cli\u003e\u003cp\u003e\u003ca href=\"\/products\/biofargo-xbai-gmp-grade\"\u003e\u003cstrong\u003eBiofargo XbaI, GMP Grade\u003c\/strong\u003e\u003c\/a\u003e — Type II, palindromic site; 20 U\/µL, 20,000 U.\u003c\/p\u003e\u003c\/li\u003e\n  \u003cli\u003e\u003cp\u003e\u003ca href=\"\/products\/biofargo-xhoi-gmp-grade\"\u003e\u003cstrong\u003eBiofargo XhoI, GMP Grade\u003c\/strong\u003e\u003c\/a\u003e — Type II, palindromic site; 20 U\/µL, 20,000 U.\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca target=\"_blank\" rel=\"noopener\" href=\"https:\/\/biofargo.com\/pages\/contact\"\u003e\u003cstrong\u003eRequest Datasheet\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n","brand":"Biofargo","offers":[{"title":"Default Title","offer_id":53432385437877,"sku":"BF-E4003","price":1050.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/biofargo.com\/products\/biofargo-bspqi-gmp-grade","provider":"Biofargo","version":"1.0","type":"link"}