Synonym(s): N,N-Bis(2-hydroxyethyl)glycine

Description

In biological experiments, it is an important pH stabilizing reagent. Usually, an appropriate weak acid is mixed with its conjugate base to obtain a suitable pH value. Most biological reactions occur under neutral conditions, and the pH is generally selected between 6-8. Meanwhile, the effective buffering range of the buffer is required to be between 6-8 as well. In addition, it is required that the acidic and basic forms of the buffer do not undergo chelation with certain metal ions.

Specifications

Item	Value
Chemical Name	N,N-Bis(2-Hydroxyethyl)Glycine
Appearance	White Crystal
CAS No.	150-25-4
Molecular Formula	C6H13NO4
Molecular Weight	163.0953
Grade	Biochemical Grade
Purity	≥99.0%
Heavy Metals	≤5ppm
Loss on Drying	≤0.5%
Water Solubility	Transparent
pH (1M Aqueous Solution at 20℃)	4.0-6.0
UV Absorbance (260nm, 1mmol/L, 1cm)	≤0.07
UV Absorbance (280nm, 1mmol/L, 1cm)	≤0.04
Storage / Handling	Store at Room Temperature.

Features

High-purity biochemical grade with ≥99% assay for reliable experimental performance.
White crystalline powder with excellent water solubility for fast and easy buffer preparation.
Low UV absorbance at 260 nm and 280 nm, suitable for spectrophotometric and UV-sensitive assays.
Stable physicochemical properties with low heavy metal content and minimal loss on drying.
Effective buffer capacity near neutral to slightly alkaline pH, offering stable performance in a wide range of biological experiments.
Convenient room-temperature storage with strong stability under standard laboratory conditions.

Applications

Protein research: Suitable for protein purification, crystallization, stability studies, and structural analysis.
Enzyme assays: Provides controlled pH environments for enzymatic reactions without interfering with metal-dependent enzymes.
Electrophoresis: Used in buffer systems for PAGE and other protein or peptide separation techniques.
Chromatography: Applicable in ion-exchange and general biochemical chromatography workflows.
General laboratory use: Ideal for preparing biological buffers for molecular biology, biochemistry, and cell-free systems requiring stable pH around 7.6–9.0.

Safety Disclaimer

This product is intended for laboratory research use only. Not for drug, human, or veterinary use. Review the Safety Data Sheet (SDS) provided by BioFargo for detailed handling, storage, hazard, and disposal information. Use appropriate personal protective equipment (PPE) including gloves, lab coat, and eye protection when handling. Dispose of waste and residual buffer in accordance with local, state, and federal regulations. Ensure proper ventilation if preparing large volumes or heating solutions.

Documents

SDS

BICINE 99% Biochemical Grade