{"product_id":"bf-snu466-human-glioblastoma-cell-line","title":"BF-SNU466 Human Glioblastoma Cell Line","description":"\u003csection\u003e\n  \u003ch2\u003eDescription\u003c\/h2\u003e\n  \u003cp\u003eLaboratories selecting a human glioblastoma cell line need clearly documented information on tissue origin, cellular morphology, growth pattern, culture medium, passaging, and cell identity before beginning routine culture. BF-SNU466 is a human brain-derived glioblastoma cell line established from a 61-year-old Asian male. The cells have spindle morphology and grow as a monolayer.\u003c\/p\u003e\n  \u003cp\u003eThe BF-SNU466 cell line has a reported population doubling time of 54 hours and a recommended split ratio of 1:4. Its specified culture system uses RPMI 1640 containing L-glutamine, HEPES, and sodium bicarbonate, supplemented with fetal bovine serum. The documented culture conditions, subculturing procedure, freezing medium, and STR profile below provide laboratories with the reference information needed to plan the routine handling of BF-SNU466 cells.\u003c\/p\u003e\n\u003c\/section\u003e\n\n\u003csection\u003e\n  \u003ch2\u003eSpecification\u003c\/h2\u003e\n\n  \u003cdiv style=\"max-width:100%;overflow-x:auto;-webkit-overflow-scrolling:touch;\"\u003e\n    \u003ctable style=\"width:100%;min-width:620px;border-collapse:collapse;\"\u003e\n      \u003ctbody\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px;text-align:left;border:1px solid #dddddd;\"\u003eProduct Name\u003c\/th\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eBF-SNU466 Human Glioblastoma Cell Line\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px;text-align:left;border:1px solid #dddddd;\"\u003eCatalog Number\u003c\/th\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eBF-SNU466\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px;text-align:left;border:1px solid #dddddd;\"\u003eSpecies\u003c\/th\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eHuman\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px;text-align:left;border:1px solid #dddddd;\"\u003eDonor\u003c\/th\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eAsian male, 61 years old\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px;text-align:left;border:1px solid #dddddd;\"\u003eTissue of Origin\u003c\/th\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eBrain\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px;text-align:left;border:1px solid #dddddd;\"\u003eHistopathology\u003c\/th\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eGlioblastoma\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px;text-align:left;border:1px solid #dddddd;\"\u003eCellular Morphology\u003c\/th\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eSpindle\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px;text-align:left;border:1px solid #dddddd;\"\u003eGrowth Pattern\u003c\/th\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eMonolayer\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px;text-align:left;border:1px solid #dddddd;\"\u003ePopulation Doubling Time\u003c\/th\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003e54 hours\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px;text-align:left;border:1px solid #dddddd;\"\u003eSplit Ratio\u003c\/th\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003e1:4\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px;text-align:left;border:1px solid #dddddd;\"\u003eOriginal Culture Medium\u003c\/th\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eRPMI 1640 containing L-glutamine (300 mg\/L), 25 mM HEPES, and 25 mM NaHCO3, 90%; heat-inactivated fetal bovine serum, 10%\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px;text-align:left;border:1px solid #dddddd;\"\u003eCulture Medium\u003c\/th\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eRPMI 1640 containing L-glutamine (300 mg\/L), 25 mM HEPES, and 25 mM NaHCO3, 90%; fetal bovine serum, 10%\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px;text-align:left;border:1px solid #dddddd;\"\u003eFreeze Medium\u003c\/th\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eRPMI 1640, 52.5%; FBS, 40%; DMSO, 7.5%\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px;text-align:left;border:1px solid #dddddd;\"\u003eMedia Change\u003c\/th\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eEvery 2 to 3 days, depending on cell density\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px;text-align:left;border:1px solid #dddddd;\"\u003eDepositor\u003c\/th\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eJae-Gahb Park, M.D.\u003c\/td\u003e\n        \u003c\/tr\u003e\n      \u003c\/tbody\u003e\n    \u003c\/table\u003e\n  \u003c\/div\u003e\n\n  \u003ch3\u003eBF-SNU466 STR Profile\u003c\/h3\u003e\n\n  \u003cdiv style=\"max-width:100%;overflow-x:auto;-webkit-overflow-scrolling:touch;\"\u003e\n    \u003ctable style=\"width:100%;min-width:500px;border-collapse:collapse;\"\u003e\n      \u003cthead\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"col\" style=\"padding:10px;text-align:left;border:1px solid #dddddd;\"\u003eSTR Locus\u003c\/th\u003e\n          \u003cth scope=\"col\" style=\"padding:10px;text-align:left;border:1px solid #dddddd;\"\u003eAlleles\u003c\/th\u003e\n        \u003c\/tr\u003e\n      \u003c\/thead\u003e\n      \u003ctbody\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eD3S1358\u003c\/td\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003e15, 18\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003evWA\u003c\/td\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003e17, 18\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eFGA\u003c\/td\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003e22, 24\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eAmelogenin\u003c\/td\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eX, Y\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eTH01\u003c\/td\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003e6, 9\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eTPOX\u003c\/td\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003e9, 11\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eCSF1P0\u003c\/td\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003e12\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eD5S818\u003c\/td\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003e7, 12\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eD13S317\u003c\/td\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003e8, 12\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eD7S820\u003c\/td\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003e10, 12\u003c\/td\u003e\n        \u003c\/tr\u003e\n      \u003c\/tbody\u003e\n    \u003c\/table\u003e\n  \u003c\/div\u003e\n\u003c\/section\u003e\n\n\u003csection\u003e\n  \u003ch2\u003eFeatures\u003c\/h2\u003e\n  \u003cul\u003e\n    \u003cli\u003eHuman glioblastoma cell line\u003c\/li\u003e\n    \u003cli\u003eDerived from brain tissue\u003c\/li\u003e\n    \u003cli\u003eDonor was a 61-year-old Asian male\u003c\/li\u003e\n    \u003cli\u003eSpindle cellular morphology\u003c\/li\u003e\n    \u003cli\u003eMonolayer growth pattern\u003c\/li\u003e\n    \u003cli\u003eReported population doubling time of 54 hours\u003c\/li\u003e\n    \u003cli\u003eRecommended split ratio of 1:4\u003c\/li\u003e\n    \u003cli\u003eRPMI 1640-based culture medium\u003c\/li\u003e\n    \u003cli\u003eDocumented 10-locus STR profile\u003c\/li\u003e\n  \u003c\/ul\u003e\n\u003c\/section\u003e\n\n\u003csection\u003e\n  \u003ch2\u003eApplication\u003c\/h2\u003e\n  \u003cp\u003eBF-SNU466 cells are intended for research involving a human brain-derived glioblastoma cell line with spindle morphology and a monolayer growth pattern.\u003c\/p\u003e\n\n  \u003ch3\u003eSubculturing Procedure\u003c\/h3\u003e\n  \u003col\u003e\n    \u003cli\u003eRemove the culture medium.\u003c\/li\u003e\n    \u003cli\u003eAdd fresh 0.25% trypsin and 0.02% EDTA solution.\u003c\/li\u003e\n    \u003cli\u003eKeep the culture flask at 37°C for 3 to 5 minutes.\u003c\/li\u003e\n    \u003cli\u003eAdd culture medium and collect the cells.\u003c\/li\u003e\n    \u003cli\u003eTransfer the cell suspension into a 15 mL tube and centrifuge.\u003c\/li\u003e\n    \u003cli\u003eAspirate the medium.\u003c\/li\u003e\n    \u003cli\u003eResuspend the cell pellet with culture medium and dispense the cells into a culture flask.\u003c\/li\u003e\n  \u003c\/ol\u003e\n\n  \u003ch3\u003eMedia Change\u003c\/h3\u003e\n  \u003cp\u003eRemove two-thirds of the medium and add fresh medium every 2 to 3 days, depending on cell density.\u003c\/p\u003e\n\u003c\/section\u003e\n\n\u003csection\u003e\n  \u003ch2\u003eFAQ\u003c\/h2\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhat is the BF-SNU466 cell line?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eBF-SNU466 is a human glioblastoma cell line derived from brain tissue. The cells have spindle morphology and grow as a monolayer.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhat is the catalog number for this human glioblastoma cell line?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eThe catalog number is BF-SNU466.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhat is the origin of BF-SNU466 cells?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eBF-SNU466 cells were derived from the brain of a 61-year-old Asian male. The reported histopathology is glioblastoma.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhat morphology do BF-SNU466 cells have?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eBF-SNU466 cells have spindle cellular morphology and a monolayer growth pattern.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhat medium is used to culture BF-SNU466 cells?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eThe specified culture medium contains 90% RPMI 1640 with L-glutamine at 300 mg\/L, 25 mM HEPES, and 25 mM NaHCO3, supplemented with 10% fetal bovine serum.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhat original medium is specified for BF-SNU466 cells?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eThe specified original medium contains 90% RPMI 1640 with L-glutamine at 300 mg\/L, 25 mM HEPES, and 25 mM NaHCO3, supplemented with 10% heat-inactivated fetal bovine serum.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhat is the population doubling time of BF-SNU466 cells?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eThe reported population doubling time of the BF-SNU466 cell line is 54 hours.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhat split ratio is recommended for BF-SNU466 cells?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eThe recommended split ratio for BF-SNU466 cells is 1:4.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eHow often should the BF-SNU466 culture medium be changed?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eTwo-thirds of the medium should be removed and replaced with fresh medium every 2 to 3 days, depending on cell density.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eHow are BF-SNU466 cells subcultured?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eRemove the medium and add fresh 0.25% trypsin with 0.02% EDTA. Keep the flask at 37°C for 3 to 5 minutes, add culture medium, and collect the cells. Transfer the suspension to a 15 mL tube, centrifuge, aspirate the medium, resuspend the pellet, and dispense the cells into a culture flask.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhat freezing medium is specified for BF-SNU466 cells?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eThe specified freeze medium contains 52.5% RPMI 1640, 40% FBS, and 7.5% DMSO.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eIs an STR profile available for BF-SNU466 cells?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eYes. The documented loci are D3S1358, vWA, FGA, Amelogenin, TH01, TPOX, CSF1P0, D5S818, D13S317, and D7S820. The corresponding allele values are provided in the specification table.\u003c\/p\u003e\n  \u003c\/details\u003e\n\u003c\/section\u003e","brand":"Biofargo","offers":[{"title":"Frozen","offer_id":53189192253621,"sku":"BF-SNU466","price":0.0,"currency_code":"USD","in_stock":true},{"title":"Culture","offer_id":53189192286389,"sku":"BF-SNU467","price":0.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/biofargo.com\/products\/bf-snu466-human-glioblastoma-cell-line","provider":"Biofargo","version":"1.0","type":"link"}