{"product_id":"bf-snu333-human-renal-cell-carcinoma-cell-line","title":"BF-SNU333 Human Renal Cell Carcinoma Cell Line","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003csection\u003e\n\u003cp\u003eLaboratories working with human renal cell carcinoma cells need clearly documented information on tissue origin, morphology, growth pattern, culture medium, passaging, and cell identity before beginning routine culture. The BF-SNU333 cell line is derived from the kidney of a 34-year-old Asian male with renal cell carcinoma. These human kidney cancer cells display epithelial morphology and grow as a monolayer.\u003c\/p\u003e\n\u003cp\u003eThis BF-SNU333 human renal cell carcinoma cell line, catalog number BF-SNU333, has a reported population doubling time of 76 hours and a recommended split ratio of 1:3. The specified original culture medium consists of 90% RPMI 1640 containing L-glutamine, HEPES, and sodium bicarbonate, supplemented with 10% heat-inactivated fetal bovine serum. The documented culture information and STR profile below allow researchers to review the characteristics of BF-SNU333 cells before use.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection\u003e\n\u003ch2\u003eSpecification\u003c\/h2\u003e\n\u003cdiv style=\"max-width: 100%; overflow-x: auto; -webkit-overflow-scrolling: touch;\"\u003e\n\u003ctable style=\"width: 100%; min-width: 620px; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"padding: 10px; text-align: left; border: 1px solid #dddddd;\"\u003eProduct Name\u003c\/th\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #dddddd;\"\u003eBF-SNU333 Cell Line\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"padding: 10px; text-align: left; border: 1px solid #dddddd;\"\u003eCatalog Number\u003c\/th\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #dddddd;\"\u003eBF-SNU333\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"padding: 10px; text-align: left; border: 1px solid #dddddd;\"\u003eSpecies\u003c\/th\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #dddddd;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"padding: 10px; text-align: left; border: 1px solid #dddddd;\"\u003eDonor\u003c\/th\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #dddddd;\"\u003eAsian male, 34 years old\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"padding: 10px; text-align: left; border: 1px solid #dddddd;\"\u003eTissue of Origin\u003c\/th\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #dddddd;\"\u003eKidney\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"padding: 10px; text-align: left; border: 1px solid #dddddd;\"\u003eHistopathology\u003c\/th\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #dddddd;\"\u003eRenal cell carcinoma\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"padding: 10px; text-align: left; border: 1px solid #dddddd;\"\u003eCellular Morphology\u003c\/th\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #dddddd;\"\u003eEpithelial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"padding: 10px; text-align: left; border: 1px solid #dddddd;\"\u003eGrowth Pattern\u003c\/th\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #dddddd;\"\u003eMonolayer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"padding: 10px; text-align: left; border: 1px solid #dddddd;\"\u003ePopulation Doubling Time\u003c\/th\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #dddddd;\"\u003e76 hours\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"padding: 10px; text-align: left; border: 1px solid #dddddd;\"\u003eSplit Ratio\u003c\/th\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #dddddd;\"\u003e1:3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"padding: 10px; text-align: left; border: 1px solid #dddddd;\"\u003eOriginal Culture Medium\u003c\/th\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #dddddd;\"\u003eRPMI 1640 containing L-glutamine (300 mg\/L), 25 mM HEPES, and 25 mM NaHCO3, 90%; heat-inactivated fetal bovine serum, 10%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"padding: 10px; text-align: left; border: 1px solid #dddddd;\"\u003eFreeze Medium\u003c\/th\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #dddddd;\"\u003eRPMI 1640, 52.5%; FBS, 40%; DMSO, 7.5%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"padding: 10px; text-align: left; border: 1px solid #dddddd;\"\u003eMedia Change\u003c\/th\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #dddddd;\"\u003eEvery 2 to 3 days, depending on cell density\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"padding: 10px; text-align: left; border: 1px solid #dddddd;\"\u003eDepositor\u003c\/th\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #dddddd;\"\u003eJae-Gahb Park, M.D.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3\u003eBF-SNU333 STR Profile\u003c\/h3\u003e\n\u003cdiv style=\"max-width: 100%; overflow-x: auto; -webkit-overflow-scrolling: touch;\"\u003e\n\u003ctable style=\"width: 100%; min-width: 500px; border-collapse: collapse;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth scope=\"col\" style=\"padding: 10px; text-align: left; border: 1px solid #dddddd;\"\u003eSTR Locus\u003c\/th\u003e\n\u003cth scope=\"col\" style=\"padding: 10px; text-align: left; border: 1px solid #dddddd;\"\u003eAlleles\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #dddddd;\"\u003eD3S1358\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #dddddd;\"\u003e17\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #dddddd;\"\u003evWA\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #dddddd;\"\u003e17\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #dddddd;\"\u003eFGA\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #dddddd;\"\u003e22, 23\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #dddddd;\"\u003eAmelogenin\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #dddddd;\"\u003eX, Y\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #dddddd;\"\u003eTH01\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #dddddd;\"\u003e7, 9\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #dddddd;\"\u003eTPOX\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #dddddd;\"\u003e8\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #dddddd;\"\u003eCSF1P0\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #dddddd;\"\u003e10, 11\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #dddddd;\"\u003eD5S818\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #dddddd;\"\u003e11, 13\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #dddddd;\"\u003eD13S317\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #dddddd;\"\u003e11\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #dddddd;\"\u003eD7S820\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #dddddd;\"\u003e9, 13\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eHuman renal cell carcinoma cell line\u003c\/li\u003e\n\u003cli\u003eDerived from kidney tissue\u003c\/li\u003e\n\u003cli\u003eEpithelial cellular morphology\u003c\/li\u003e\n\u003cli\u003eMonolayer growth pattern\u003c\/li\u003e\n\u003cli\u003eReported population doubling time of 76 hours\u003c\/li\u003e\n\u003cli\u003eRecommended split ratio of 1:3\u003c\/li\u003e\n\u003cli\u003eRPMI 1640-based original culture medium\u003c\/li\u003e\n\u003cli\u003eDocumented 10-locus STR profile\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003csection\u003e\n\u003ch2\u003eApplication\u003c\/h2\u003e\n\u003cp\u003eBF-SNU333 cells are intended for research involving a human kidney-derived renal cell carcinoma cell line with epithelial morphology and a monolayer growth pattern.\u003c\/p\u003e\n\u003ch3\u003eSubculturing Procedure\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003eRemove the culture medium.\u003c\/li\u003e\n\u003cli\u003eAdd fresh 0.25% trypsin and 0.02% EDTA solution.\u003c\/li\u003e\n\u003cli\u003eKeep the culture flask at 37°C for 3 to 5 minutes.\u003c\/li\u003e\n\u003cli\u003eAdd culture medium and collect the cells.\u003c\/li\u003e\n\u003cli\u003eTransfer the medium into a 15 mL tube and centrifuge.\u003c\/li\u003e\n\u003cli\u003eAspirate the medium.\u003c\/li\u003e\n\u003cli\u003eResuspend the cell pellet with culture medium and dispense it into the culture flask.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch3\u003eMedia Change\u003c\/h3\u003e\n\u003cp\u003eRemove two-thirds of the medium and add fresh medium every 2 to 3 days, depending on cell density.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eWhat is the BF-SNU333 cell line?\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cp\u003eBF-SNU333 is a human renal cell carcinoma cell line derived from kidney tissue. The cells have epithelial morphology and grow as a monolayer.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eWhat is the catalog number for the BF-SNU333 cell line?\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cp\u003eThe catalog number for this BF-SNU333 cell line is BF-SNU333.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eWhat is the origin of BF-SNU333 cells?\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cp\u003eBF-SNU333 cells were derived from the kidney of a 34-year-old Asian male. The reported histopathology is renal cell carcinoma.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eWhat morphology and growth pattern do BF-SNU333 cells have?\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cp\u003eBF-SNU333 cells have epithelial cellular morphology and a monolayer growth pattern.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eWhat medium is specified for culturing BF-SNU333 cells?\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cp\u003eThe specified original medium is 90% RPMI 1640 containing L-glutamine at 300 mg\/L, 25 mM HEPES, and 25 mM NaHCO3, supplemented with 10% heat-inactivated fetal bovine serum.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eWhat is the population doubling time of BF-SNU333 cells?\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cp\u003eThe reported population doubling time of the BF-SNU333 cell line is 76 hours.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eWhat split ratio is recommended for BF-SNU333 cells?\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cp\u003eThe recommended split ratio for BF-SNU333 cells is 1:3.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eHow often should the BF-SNU333 culture medium be changed?\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cp\u003eTwo-thirds of the medium should be removed and replaced with fresh medium every 2 to 3 days, depending on cell density.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eHow are BF-SNU333 cells subcultured?\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cp\u003eRemove the medium and add fresh 0.25% trypsin with 0.02% EDTA. Keep the flask at 37°C for 3 to 5 minutes, add culture medium, and collect the cells. Transfer the suspension to a 15 mL tube, centrifuge, aspirate the medium, resuspend the pellet, and dispense the cells into a culture flask.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eWhat freezing medium is specified for BF-SNU333 cells?\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cp\u003eThe specified freeze medium contains 52.5% RPMI 1640, 40% FBS, and 7.5% DMSO.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eIs an STR profile available for BF-SNU333 cells?\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cp\u003eYes. The documented loci are D3S1358, vWA, FGA, Amelogenin, TH01, TPOX, CSF1P0, D5S818, D13S317, and D7S820. The corresponding allele values are provided in the specification table.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003c\/section\u003e\n\u003cp\u003e```\u003c\/p\u003e","brand":"Biofargo","offers":[{"title":"Frozen","offer_id":53189191041205,"sku":"BF-SNU333","price":0.0,"currency_code":"USD","in_stock":true},{"title":"Culture","offer_id":53189191073973,"sku":"BF-SNU334","price":0.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/biofargo.com\/products\/bf-snu333-human-renal-cell-carcinoma-cell-line","provider":"Biofargo","version":"1.0","type":"link"}