{"product_id":"bf-hcc2108-human-lung-adenocarcinoma-cell-line","title":"BF-HCC2108 Human Lung Adenocarcinoma Cell Line","description":"\u003csection\u003e\n  \u003ch2\u003eDescription\u003c\/h2\u003e\n  \u003cp\u003eLaboratories selecting a human lung adenocarcinoma cell line need clearly documented information on tissue origin, cellular morphology, growth pattern, culture medium, freezing medium, and cell identity before beginning routine culture. BF-HCC2108 is a human lung-derived adenocarcinoma cell line established from a 59-year-old male. The cells have epithelial morphology and grow as a monolayer.\u003c\/p\u003e\n  \u003cp\u003eThe BF-HCC2108 cell line uses an RPMI 1640-based culture medium supplemented with heat-inactivated fetal bovine serum. The documented culture information includes the medium formulation, freezing medium, media-change interval, available subculture information, and a 10-locus STR profile. These specifications allow laboratories to review the reported characteristics of BF-HCC2108 cells before use.\u003c\/p\u003e\n\u003c\/section\u003e\n\n\u003csection\u003e\n  \u003ch2\u003eSpecification\u003c\/h2\u003e\n\n  \u003cdiv style=\"max-width:100%;overflow-x:auto;-webkit-overflow-scrolling:touch;\"\u003e\n    \u003ctable style=\"width:100%;min-width:620px;border-collapse:collapse;\"\u003e\n      \u003ctbody\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px;text-align:left;border:1px solid #dddddd;\"\u003eProduct Name\u003c\/th\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eBF-HCC2108 Human Lung Adenocarcinoma Cell Line\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px;text-align:left;border:1px solid #dddddd;\"\u003eCatalog Number\u003c\/th\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eBF-HCC2108\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px;text-align:left;border:1px solid #dddddd;\"\u003eSpecies\u003c\/th\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eHuman\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px;text-align:left;border:1px solid #dddddd;\"\u003eDonor\u003c\/th\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eMale, 59 years old\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px;text-align:left;border:1px solid #dddddd;\"\u003eTissue of Origin\u003c\/th\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eLung\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px;text-align:left;border:1px solid #dddddd;\"\u003eHistopathology\u003c\/th\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eAdenocarcinoma\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px;text-align:left;border:1px solid #dddddd;\"\u003eCellular Morphology\u003c\/th\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eEpithelial\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px;text-align:left;border:1px solid #dddddd;\"\u003eGrowth Pattern\u003c\/th\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eMonolayer\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px;text-align:left;border:1px solid #dddddd;\"\u003eOriginal Culture Medium\u003c\/th\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eRPMI 1640 containing L-glutamine (300 mg\/L), 25 mM HEPES, and 25 mM NaHCO3, 90%; heat-inactivated fetal bovine serum, 10%\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px;text-align:left;border:1px solid #dddddd;\"\u003eCulture Medium\u003c\/th\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eRPMI 1640 containing L-glutamine (300 mg\/L), 25 mM HEPES, and 25 mM NaHCO3, 90%; heat-inactivated fetal bovine serum, 10%\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px;text-align:left;border:1px solid #dddddd;\"\u003eFreeze Medium\u003c\/th\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eRPMI 1640, 52.5%; FBS, 40%; DMSO, 7.5%\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px;text-align:left;border:1px solid #dddddd;\"\u003eMedia Change\u003c\/th\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eEvery 2 to 3 days, depending on cell density\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px;text-align:left;border:1px solid #dddddd;\"\u003eAvailable Subculture Information\u003c\/th\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eRemove the medium, add fresh 0.25% trypsin and 0.02% EDTA solution, and keep the culture flask at 37°C. Additional subculture steps are not listed in the provided product information.\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"padding:10px;text-align:left;border:1px solid #dddddd;\"\u003eDepositor\u003c\/th\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eGazdar AF\u003c\/td\u003e\n        \u003c\/tr\u003e\n      \u003c\/tbody\u003e\n    \u003c\/table\u003e\n  \u003c\/div\u003e\n\n  \u003ch3\u003eBF-HCC2108 STR Profile\u003c\/h3\u003e\n\n  \u003cdiv style=\"max-width:100%;overflow-x:auto;-webkit-overflow-scrolling:touch;\"\u003e\n    \u003ctable style=\"width:100%;min-width:500px;border-collapse:collapse;\"\u003e\n      \u003cthead\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"col\" style=\"padding:10px;text-align:left;border:1px solid #dddddd;\"\u003eSTR Locus\u003c\/th\u003e\n          \u003cth scope=\"col\" style=\"padding:10px;text-align:left;border:1px solid #dddddd;\"\u003eAlleles\u003c\/th\u003e\n        \u003c\/tr\u003e\n      \u003c\/thead\u003e\n      \u003ctbody\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eD3S1358\u003c\/td\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003e15\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003evWA\u003c\/td\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003e16\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eFGA\u003c\/td\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003e22\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eAmelogenin\u003c\/td\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eX\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eTH01\u003c\/td\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003e7, 9\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eTPOX\u003c\/td\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003e10, 11\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eCSF1P0\u003c\/td\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003e11\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eD5S818\u003c\/td\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003e11, 13\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eD13S317\u003c\/td\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003e11\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003eD7S820\u003c\/td\u003e\n          \u003ctd style=\"padding:10px;border:1px solid #dddddd;\"\u003e9, 11\u003c\/td\u003e\n        \u003c\/tr\u003e\n      \u003c\/tbody\u003e\n    \u003c\/table\u003e\n  \u003c\/div\u003e\n\u003c\/section\u003e\n\n\u003csection\u003e\n  \u003ch2\u003eFeatures\u003c\/h2\u003e\n  \u003cul\u003e\n    \u003cli\u003eHuman lung adenocarcinoma cell line\u003c\/li\u003e\n    \u003cli\u003eDerived from lung tissue\u003c\/li\u003e\n    \u003cli\u003eDonor was a 59-year-old male\u003c\/li\u003e\n    \u003cli\u003eEpithelial cellular morphology\u003c\/li\u003e\n    \u003cli\u003eMonolayer growth pattern\u003c\/li\u003e\n    \u003cli\u003eRPMI 1640-based culture medium\u003c\/li\u003e\n    \u003cli\u003eSpecified freezing medium containing RPMI 1640, FBS, and DMSO\u003c\/li\u003e\n    \u003cli\u003eMedia change every 2 to 3 days, depending on cell density\u003c\/li\u003e\n    \u003cli\u003eDocumented 10-locus STR profile\u003c\/li\u003e\n  \u003c\/ul\u003e\n\u003c\/section\u003e\n\n\u003csection\u003e\n  \u003ch2\u003eApplication\u003c\/h2\u003e\n  \u003cp\u003eBF-HCC2108 cells are intended for research involving a human lung-derived adenocarcinoma cell line with epithelial morphology and a monolayer growth pattern.\u003c\/p\u003e\n\n  \u003ch3\u003eCulture Information\u003c\/h3\u003e\n  \u003cp\u003eThe specified culture medium contains 90% RPMI 1640 with L-glutamine at 300 mg\/L, 25 mM HEPES, and 25 mM NaHCO3, supplemented with 10% heat-inactivated fetal bovine serum.\u003c\/p\u003e\n\n  \u003ch3\u003eMedia Change\u003c\/h3\u003e\n  \u003cp\u003eRemove two-thirds of the medium and add fresh medium every 2 to 3 days, depending on cell density.\u003c\/p\u003e\n\n  \u003ch3\u003eAvailable Subculture Information\u003c\/h3\u003e\n  \u003cp\u003eRemove the culture medium, add fresh 0.25% trypsin and 0.02% EDTA solution, and keep the culture flask at 37°C. Additional subculture steps are not listed in the provided product information.\u003c\/p\u003e\n\u003c\/section\u003e\n\n\u003csection\u003e\n  \u003ch2\u003eFAQ\u003c\/h2\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhat is the BF-HCC2108 cell line?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eBF-HCC2108 is a human lung adenocarcinoma cell line derived from lung tissue. The cells have epithelial morphology and grow as a monolayer.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhat is the catalog number for this human lung adenocarcinoma cell line?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eThe catalog number is BF-HCC2108.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhat is the origin of BF-HCC2108 cells?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eBF-HCC2108 cells were derived from the lung of a 59-year-old male. The reported histopathology is adenocarcinoma.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhat morphology do BF-HCC2108 cells have?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eBF-HCC2108 cells have epithelial cellular morphology and a monolayer growth pattern.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhat medium is used to culture BF-HCC2108 cells?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eThe specified medium contains 90% RPMI 1640 with L-glutamine at 300 mg\/L, 25 mM HEPES, and 25 mM NaHCO3, supplemented with 10% heat-inactivated fetal bovine serum.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhat freezing medium is specified for BF-HCC2108 cells?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eThe specified freezing medium contains 52.5% RPMI 1640, 40% FBS, and 7.5% DMSO.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eHow often should the BF-HCC2108 culture medium be changed?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eTwo-thirds of the medium should be removed and replaced with fresh medium every 2 to 3 days, depending on cell density.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhat subculture information is available for BF-HCC2108 cells?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eThe available information states that the medium should be removed, fresh 0.25% trypsin and 0.02% EDTA solution should be added, and the culture flask should be kept at 37°C. Additional subculture steps are not listed in the provided product information.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eIs a doubling time specified for BF-HCC2108 cells?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eNo population doubling time is listed in the provided product information.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eIs a split ratio specified for BF-HCC2108 cells?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eNo recommended split ratio is listed in the provided product information.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eIs an STR profile available for BF-HCC2108 cells?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eYes. The documented loci are D3S1358, vWA, FGA, Amelogenin, TH01, TPOX, CSF1P0, D5S818, D13S317, and D7S820. The corresponding allele values are provided in the specification table.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhat Amelogenin result is reported for BF-HCC2108 cells?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eThe reported Amelogenin result is X.\u003c\/p\u003e\n  \u003c\/details\u003e\n\u003c\/section\u003e","brand":"Biofargo","offers":[{"title":"Frozen","offer_id":53189199397045,"sku":"BF-HCC2108","price":0.0,"currency_code":"USD","in_stock":true},{"title":"Culture","offer_id":53189199429813,"sku":"BF-HCC2109","price":0.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/biofargo.com\/products\/bf-hcc2108-human-lung-adenocarcinoma-cell-line","provider":"Biofargo","version":"1.0","type":"link"}