{"product_id":"bf-001agbm-sheep-anti-rat-isolated-gbm-serum","title":"Bf-001AGBM Sheep Anti-Rat Isolated GBM Serum","description":"\u003carticle\u003e\u003cheader\u003e\u003c\/header\u003e\n\u003csection\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eEstablishing an experimental anti-GBM nephritis model requires an antiserum that targets the rat glomerular basement membrane and provides sufficient material for a defined number of animals. Biofargo Bf-001AGBM is a sheep anti-rat isolated glomerular basement membrane serum supplied for the induction of passive anti-GBM nephritis in rats.\u003c\/p\u003e\n\u003cp\u003eThis nephrotoxic anti-GBM serum is supplied as 25 mL in 0.02 M phosphate-buffered saline at pH 7.3. One bottle contains sufficient antibody to induce passive anti-GBM nephritis in at least 25 rats weighing 175–200 g when used according to the stated directions.\u003c\/p\u003e\n\u003cp\u003eThe rat anti-GBM nephritis model is associated with mesangial proliferation, inflammatory cell vasculitis, glomerulosclerosis and renal fibrosis. The disease response is dose-dependent, so the complete dose must be delivered. Because antiserum lots, rat sources and experimental requirements can vary, a dose-response study should be performed to establish the desired disease severity for each lot.\u003c\/p\u003e\n\u003cp\u003eBf-001AGBM replaces the previous sheep anti-whole-rat-glomeruli serum. Both products target rat GBM and produce the same type of disease model. The current product has not been validated or recommended for use in mice.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection\u003e\n\u003ch2\u003eSpecification\u003c\/h2\u003e\n\u003cdiv style=\"max-width: 100%; overflow-x: auto; -webkit-overflow-scrolling: touch;\"\u003e\n\u003ctable\u003e\n\u003ccaption\u003eBiofargo Bf-001AGBM product specifications\u003c\/caption\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth scope=\"col\"\u003eParameter\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eSpecification\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eBrand\u003c\/th\u003e\n\u003ctd\u003eBiofargo\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eCatalog number\u003c\/th\u003e\n\u003ctd\u003eBf-001AGBM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eProduct name\u003c\/th\u003e\n\u003ctd\u003eSheep Anti-Rat Isolated Glomerular Basement Membrane Serum\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eAlternative name\u003c\/th\u003e\n\u003ctd\u003eSheep Anti-Rat Anti-GBM Serum\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eProduct type\u003c\/th\u003e\n\u003ctd\u003eNephrotoxic antiserum\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eHost species\u003c\/th\u003e\n\u003ctd\u003eSheep\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eTarget species\u003c\/th\u003e\n\u003ctd\u003eRat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eIsolated rat glomerular basement membrane\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eResearch model\u003c\/th\u003e\n\u003ctd\u003ePassive anti-GBM nephritis and immune-mediated glomerulonephritis\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eForm\u003c\/th\u003e\n\u003ctd\u003eSerum\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eVolume\u003c\/th\u003e\n\u003ctd\u003e25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eBuffer\u003c\/th\u003e\n\u003ctd\u003e0.02 M phosphate-buffered saline\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003epH\u003c\/th\u003e\n\u003ctd\u003e7.3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eHeat treatment\u003c\/th\u003e\n\u003ctd\u003e56°C for 30 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eStorage\u003c\/th\u003e\n\u003ctd\u003e-20°C or below\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eFreeze-thaw instruction\u003c\/th\u003e\n\u003ctd\u003eAvoid repeated freeze-thaw cycles\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eAnimal coverage\u003c\/th\u003e\n\u003ctd\u003eSufficient to induce disease in at least 25 rats weighing 175–200 g when used according to the stated directions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eRecommended rat strain\u003c\/th\u003e\n\u003ctd\u003eSprague-Dawley\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eMouse validation\u003c\/th\u003e\n\u003ctd\u003eNot validated or recommended for mice\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eBulk quantities\u003c\/th\u003e\n\u003ctd\u003eAvailable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDefined anti-GBM target:\u003c\/strong\u003e Sheep antiserum directed against isolated rat glomerular basement membrane.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePassive anti-GBM nephritis model:\u003c\/strong\u003e Supplied for the induction of immune-mediated glomerulonephritis in rats.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e25 mL package:\u003c\/strong\u003e One bottle contains sufficient antibody for at least 25 rats weighing 175–200 g when used according to the stated directions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecified formulation:\u003c\/strong\u003e Supplied in 0.02 M phosphate-buffered saline at pH 7.3.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHeat-treated serum:\u003c\/strong\u003e Treated at 56°C for 30 minutes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFrozen storage:\u003c\/strong\u003e Store at -20°C or below and avoid repeated freeze-thaw cycles.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel-associated findings:\u003c\/strong\u003e The product page identifies mesangial proliferation, inflammatory cell vasculitis, glomerulosclerosis and renal fibrosis.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBulk supply:\u003c\/strong\u003e Bulk quantities are available.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003csection\u003e\n\u003ch2\u003eApplication\u003c\/h2\u003e\n\u003cp\u003eBiofargo Bf-001AGBM sheep anti-rat GBM serum is supplied for the induction of passive anti-GBM nephritis and immune-mediated glomerulonephritis in rats.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003ePassive anti-GBM nephritis induction\u003c\/li\u003e\n\u003cli\u003eImmune-mediated glomerulonephritis model establishment\u003c\/li\u003e\n\u003cli\u003eMesangial proliferation research\u003c\/li\u003e\n\u003cli\u003eInflammatory cell vasculitis research\u003c\/li\u003e\n\u003cli\u003eGlomerulosclerosis research\u003c\/li\u003e\n\u003cli\u003eRenal fibrosis research\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eModel Characteristics Described in the Product Data\u003c\/h3\u003e\n\u003cp\u003eFollowing administration of the anti-GBM serum, linear deposits of heterologous sheep IgG can become detectable in the glomerular basement membrane by immunofluorescence within minutes and increase in intensity during the following 3–5 days. Rat C3 can also localize in a linear pattern, and proteinuria can develop after 24 hours.\u003c\/p\u003e\n\u003cp\u003eThe autologous phase can become noticeable 8–10 days after anti-GBM antibody administration, with increased proteinuria and linear glomerular localization of rat IgG. By three weeks, the product data state that proteinuria can exceed 200 mg per 24 hours and that glomerular lesions can become apparent and increase in severity over time, leading to glomerulosclerosis.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is Biofargo Bf-001AGBM?\u003c\/summary\u003e\n\u003cp\u003eBf-001AGBM is a sheep anti-rat isolated glomerular basement membrane serum supplied for the induction of passive anti-GBM nephritis and immune-mediated glomerulonephritis in rats.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is nephrotoxic anti-GBM serum used for?\u003c\/summary\u003e\n\u003cp\u003eThis nephrotoxic antiserum is used to induce an experimental rat anti-GBM nephritis model. The product information associates the model with mesangial proliferation, inflammatory cell vasculitis, glomerulosclerosis and renal fibrosis.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat species is Bf-001AGBM produced in?\u003c\/summary\u003e\n\u003cp\u003eThe antiserum is produced in sheep and targets isolated rat glomerular basement membrane.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the package size?\u003c\/summary\u003e\n\u003cp\u003eBf-001AGBM is supplied in a 25 mL bottle.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow many rats can be studied with one bottle?\u003c\/summary\u003e\n\u003cp\u003eOne 25 mL bottle contains sufficient antibody to induce passive anti-GBM nephritis in at least 25 rats weighing 175–200 g when used according to the stated directions.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat rat strain is recommended?\u003c\/summary\u003e\n\u003cp\u003eThe product data recommend the Sprague-Dawley rat strain. Other rat strains were not tested in the referenced product data.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the recommended dose of sheep anti-rat GBM serum?\u003c\/summary\u003e\n\u003cp\u003eThe stated recommended dose is 0.4–0.5 mL per 100 g of body weight. Disease production is dose-dependent, and the complete dose must be delivered.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eShould a dose-response study be performed?\u003c\/summary\u003e\n\u003cp\u003eYes. Because antiserum lots, rat sources and investigator requirements can vary, the product data recommend performing a dose-response study to establish the desired disease severity for each lot.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat buffer is used for Bf-001AGBM?\u003c\/summary\u003e\n\u003cp\u003eThe serum is supplied in 0.02 M phosphate-buffered saline at pH 7.3.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHas the serum been heat-treated?\u003c\/summary\u003e\n\u003cp\u003eYes. The product has been heat-treated at 56°C for 30 minutes.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow should Bf-001AGBM be stored?\u003c\/summary\u003e\n\u003cp\u003eStore the sheep anti-rat GBM serum at -20°C or below and avoid repeated freeze-thaw cycles.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat should be done if a precipitate appears after thawing?\u003c\/summary\u003e\n\u003cp\u003eA slight precipitate may form after thawing. The product data instruct users to centrifuge the serum at 3,000–5,000 rpm for 30 minutes before injection.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan Bf-001AGBM be used in mice?\u003c\/summary\u003e\n\u003cp\u003eThe product has not been validated or recommended for use in mice.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat happened to the previous sheep anti-whole-rat-glomeruli serum?\u003c\/summary\u003e\n\u003cp\u003eBf-001AGBM replaces the previous sheep anti-whole-rat-glomeruli serum. Both products target rat glomerular basement membrane and produce the same type of disease model.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHas chronic disease been verified with this anti-GBM serum?\u003c\/summary\u003e\n\u003cp\u003eChronic disease was not verified in the referenced product data.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eAre bulk quantities of Bf-001AGBM available?\u003c\/summary\u003e\n\u003cp\u003eYes. Bulk quantities are available.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003c\/section\u003e\n\u003c\/article\u003e","brand":"Biofargo","offers":[{"title":"25ml","offer_id":53104693674165,"sku":"BF-001AGBM","price":850.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Bf-001AGBMSheepAnti-RatGBMSerum.jpg?v=1787879920","url":"https:\/\/biofargo.com\/products\/bf-001agbm-sheep-anti-rat-isolated-gbm-serum","provider":"Biofargo","version":"1.0","type":"link"}