Application

5-Bromo-4-chloro-3-indolyl phosphate disodium salt has been used to detect protein-antibody complexes.[1] It has also been used for tissue staining in alkaline phosphatase histochemistry.

For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.

BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.

For the colorimetric detection of alkaline phosphatase-labeled molecules.

Specifications

Grade	Ultra Pure
Formula	C8H4BrClNO4P•2Na
MW	370.43
CAS	102185-33-1
Appearance	Off-white solid
I.R.	Consistent with structure
% Water	8.5% ± 1.5%
Purity (HPLC)	Min 98%
Storage	-15~-20℃, in dark

Documents

COA

SDS

Disclaimer: For laboratory research use only.

BCIP Disodium Salt