{"product_id":"basement-membrane-matrix-ipsc-qualified","title":"Basement Membrane Matrix, iPSC-Qualified","description":"\u003c!--\nSEO TITLE:\niPSC-Qualified Basement Membrane Matrix for Feeder-Free Culture | Biofargo\nMETA DESCRIPTION:\nBiofargo iPSC-qualified basement membrane matrix (8–12 mg\/mL) for thin-layer coating in feeder-free hESC and iPSC culture and expansion. 5 mL, Research Use Only.\n--\u003e\n\u003cstyle\u003e\n.pdp-container{font-family:-apple-system,BlinkMacSystemFont,\"Segoe UI\",Roboto,Helvetica,Arial,sans-serif;color:#333333;line-height:1.6;max-width:1200px;margin:0 auto;padding:15px;}\n.pdp-container h2{font-size:24px;color:#0B3C5D;border-bottom:2px solid #328CC1;padding-bottom:8px;margin-top:40px;margin-bottom:20px;font-weight:600;}\n.pdp-container h3{font-size:18px;color:#1D2731;margin-top:30px;margin-bottom:15px;font-weight:600;}\n.pdp-container p{margin-bottom:16px;font-size:15px;color:#4A4A4A;text-align:justify;}\n.pdp-container ul,.pdp-container ol{padding-left:20px;margin-bottom:24px;}\n.pdp-container li{margin-bottom:10px;font-size:15px;color:#4A4A4A;}\n.pdp-container ul li{list-style-type:square;}\n.pdp-table-responsive{width:100%;overflow-x:auto;margin:25px 0;border:1px solid #E0E0E0;border-radius:6px;}\n.pdp-table{width:100%;border-collapse:collapse;min-width:600px;background:#FFFFFF;}\n.pdp-table td{padding:12px 16px;border-bottom:1px solid #E0E0E0;font-size:14px;}\n.pdp-table tr:nth-child(even){background:#F8F9FA;}\n.pdp-table td strong{color:#0B3C5D;}\n.pdp-container details{background:#F8F9FA;border:1px solid #E0E0E0;border-radius:6px;margin-bottom:12px;padding:14px 18px;}\n.pdp-container details[open]{background:#FFFFFF;border-color:#328CC1;}\n.pdp-container summary{font-weight:600;color:#0B3C5D;cursor:pointer;font-size:15px;}\n\u003c\/style\u003e\n\u003cdiv class=\"pdp-container\"\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eFeeder-free culture of human pluripotent stem cells depends on a consistent attachment surface. Variation in the coating matrix can affect colony attachment, morphology and expansion from one passage to the next.\u003c\/p\u003e\n\u003cp\u003eBiofargo Basement Membrane Matrix, iPSC-Qualified is a basement membrane extract prepared from mouse sarcoma tissue and supplied at a protein concentration of 8–12 mg\/mL. It is intended for thin-layer coating of culture vessels for feeder-free culture and expansion of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs).\u003c\/p\u003e\n\u003cp\u003eThe matrix is liquid at 2–8°C and gels rapidly above 10°C. Its temperature-dependent gelation is reversible, so it can be thawed, aliquoted and diluted on ice before coating.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cdiv class=\"pdp-table-responsive\"\u003e\n\u003ctable class=\"pdp-table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eBiofargo Basement Membrane Matrix, iPSC-Qualified\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eBFiPSCMat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProduct Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eBasement Membrane Matrix for Pluripotent Stem Cell Culture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThin-layer coating for feeder-free hESC\/iPSC culture and expansion\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMouse sarcoma tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProtein Concentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e8–12 mg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBuffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eDMEM containing 10 μg\/mL gentamicin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eVolume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e−80°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eShelf Life\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGrade\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eResearch Use Only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eiPSC-Qualified\u003c\/strong\u003e — Supplied for feeder-free culture and expansion of human iPSCs and ESCs.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003e8–12 mg\/mL Protein Concentration\u003c\/strong\u003e — Basement membrane extract suitable for dilution to a thin-layer coating.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eReversible Temperature-Dependent Gelation\u003c\/strong\u003e — Liquid at 2–8°C and gels above 10°C; prematurely gelled matrix can be returned to a liquid state at 4°C.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eLong Shelf Life\u003c\/strong\u003e — 24 months when stored at −80°C.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eAliquot-Friendly 5 mL Format\u003c\/strong\u003e — Thaw once, aliquot by intended use, and avoid repeated freeze–thaw cycles.\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eInstructions for Use\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eThaw the matrix overnight at 2–8°C. For best temperature control, place the vial on wet ice inside a 4°C refrigerator during thawing.\u003c\/li\u003e\n\u003cli\u003eKeep the thawed matrix on ice throughout handling. The matrix gels rapidly above 10°C.\u003c\/li\u003e\n\u003cli\u003eChoose the coating thickness and matrix concentration for your application. For feeder-free culture and expansion of hESCs and iPSCs, use a thin-layer coating, not a thick gel.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch3\u003eHandling Notes\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eStore at −80°C for long-term storage. Do not use matrix that has been kept at 4°C for more than 24 hours.\u003c\/li\u003e\n\u003cli\u003eAfter thawing, aliquot into sterile tubes according to intended use. Avoid repeated freeze–thaw cycles.\u003c\/li\u003e\n\u003cli\u003eIf the matrix gels prematurely, incubate at 4°C for 12–24 hours to restore the liquid state.\u003c\/li\u003e\n\u003cli\u003eIf the matrix looks uneven after thawing, pipette gently up and down until homogeneous.\u003c\/li\u003e\n\u003cli\u003eTo remove air bubbles, centrifuge at 5,000 × g for 1 minute at 0–4°C.\u003c\/li\u003e\n\u003cli\u003eFollow appropriate laboratory biosafety procedures and wear suitable personal protective equipment.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eFeeder-free hESC and iPSC culture\u003c\/strong\u003e — Thin-layer coating of plates and flasks for routine pluripotent stem cell maintenance.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eiPSC expansion\u003c\/strong\u003e — Attachment surface for scaling up human iPSC cultures.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eiPSC differentiation workflows\u003c\/strong\u003e — Coated surface for pluripotent stem cells before directed differentiation.\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eWhy Choose Biofargo Basement Membrane Matrix, iPSC-Qualified?\u003c\/h2\u003e\n\u003cp\u003eIt is supplied specifically for pluripotent stem cell work, with a defined concentration range, a 24-month shelf life and clear handling guidance. It pairs with Biofargo hESC\/iPSC expansion media, InstantSelect passaging reagent and PluriCryo cryopreservation solution for a complete feeder-free iPSC workflow.\u003c\/p\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cdetails\u003e\u003csummary\u003eWhat is this matrix used for?\u003c\/summary\u003e\u003cp\u003eIt is used as a thin-layer coating for feeder-free culture and expansion of human ESCs and iPSCs.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails\u003e\u003csummary\u003eWhat is the protein concentration?\u003c\/summary\u003e\u003cp\u003e8–12 mg\/mL.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails\u003e\u003csummary\u003eHow should it be stored?\u003c\/summary\u003e\u003cp\u003eStore at −80°C. Shelf life is 24 months. Do not use matrix kept at 4°C for more than 24 hours.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails\u003e\u003csummary\u003eWhat if the matrix gels before I use it?\u003c\/summary\u003e\u003cp\u003eIncubate at 4°C for 12–24 hours to return it to a liquid state.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails\u003e\u003csummary\u003eShould I use a thin layer or a thick gel?\u003c\/summary\u003e\u003cp\u003eFor feeder-free hESC\/iPSC culture and expansion, use a thin-layer coating rather than a thick gel.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails\u003e\u003csummary\u003eIs it for clinical use?\u003c\/summary\u003e\u003cp\u003eNo. For Research Use Only. Not intended for clinical use.\u003c\/p\u003e\u003c\/details\u003e\n\u003c\/div\u003e","brand":"Biofargo","offers":[{"title":"5mL","offer_id":53607105593525,"sku":"BFiPSCMat","price":249.0,"currency_code":"USD","in_stock":false}],"url":"https:\/\/biofargo.com\/products\/basement-membrane-matrix-ipsc-qualified","provider":"Biofargo","version":"1.0","type":"link"}