{"product_id":"bacterial-protein-extraction-lysis-buffer","title":"Bacterial Protein Extraction Lysis Buffer (with Tris-HCl)","description":"\u003c!-- =========================\nPRODUCT DESCRIPTION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eBacterial Protein Extraction Lysis Buffer (with Tris-HCl) is a specialized bacterial protein extraction buffer designed for efficient disruption of bacterial cells and recovery of recombinant proteins from expression systems such as \u003ci\u003eEscherichia coli\u003c\/i\u003e. During bacterial protein preparation, incomplete cell disruption, poor protein solubilization, or inefficient separation of soluble proteins and inclusion bodies can reduce downstream purification efficiency. This bacterial lysis buffer provides a reliable extraction environment to help researchers obtain bacterial protein lysates for protein purification, SDS-PAGE analysis, and biochemical studies.\u003c\/p\u003e\n\u003cp\u003eFormulated with Tris-HCl, Triton X-100, and NaCl, this bacterial protein extraction reagent supports efficient cell lysis while maintaining a suitable ionic environment for protein stability. After bacterial disruption, the lysate can be separated into soluble protein fractions and insoluble inclusion body fractions, allowing researchers to select appropriate downstream purification strategies according to protein characteristics.\u003c\/p\u003e\n\u003cp\u003eBacterial Protein Extraction Lysis Buffer (with Tris-HCl) is suitable for recombinant protein expression workflows, including His-tag protein purification, GST fusion protein preparation, and other bacterial protein production applications requiring efficient extraction of intracellular proteins.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nSPECIFICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eBacterial Protein Extraction Lysis Buffer (with Tris-HCl)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eAP0991\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eVolume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eBacterial Protein Extraction Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eTris-HCl, Triton X-100, NaCl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eBacterial cell lysis, recombinant protein extraction, protein purification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSample Types\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eE. coli and other bacterial expression systems\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eRoom temperature\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eShelf Life\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e1 year\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFEATURES\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eEfficient bacterial protein extraction:\u003c\/strong\u003e Enables effective lysis of bacterial cells to release intracellular proteins for downstream analysis and purification.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSuitable for recombinant protein workflows:\u003c\/strong\u003e Designed for protein preparation from bacterial expression systems including E. coli recombinant protein production.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSoluble protein and inclusion body recovery:\u003c\/strong\u003e Allows separation of soluble protein fractions and insoluble inclusion bodies after centrifugation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eTris-HCl buffered formulation:\u003c\/strong\u003e Provides a stable extraction environment to support consistent bacterial protein preparation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eTriton X-100 based cell lysis:\u003c\/strong\u003e Uses a nonionic detergent system to assist bacterial membrane disruption and protein release.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCompatible with protein purification workflows:\u003c\/strong\u003e Suitable for preparation of samples before affinity purification and protein characterization.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nAPPLICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eBacterial Protein Extraction Lysis Buffer (with Tris-HCl) is widely used in molecular biology and protein research workflows involving bacterial expression systems and recombinant protein production.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eE. coli Protein Extraction:\u003c\/strong\u003e Used for efficient extraction of intracellular proteins from E. coli expression strains after recombinant protein production.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Protein Preparation:\u003c\/strong\u003e Supports bacterial protein extraction workflows before purification and characterization of recombinant proteins.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProtein Purification:\u003c\/strong\u003e Provides bacterial lysates suitable for downstream affinity purification methods such as His-tag and GST fusion protein purification.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eInclusion Body Extraction:\u003c\/strong\u003e Enables recovery of insoluble protein fractions generated during high-level bacterial protein expression.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSoluble Protein Analysis:\u003c\/strong\u003e Helps researchers obtain soluble bacterial protein fractions for protein expression evaluation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSDS-PAGE Analysis:\u003c\/strong\u003e Suitable for preparing bacterial protein samples for electrophoretic separation and expression analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eWestern Blot Protein Detection:\u003c\/strong\u003e Supports preparation of bacterial protein lysates for antibody-based protein detection.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBiochemical Research:\u003c\/strong\u003e Used in bacterial protein characterization, enzyme studies, and recombinant protein development.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPROTOCOL OVERVIEW\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eProtocol Overview\u003c\/h2\u003e\n\u003cp\u003eBacterial Protein Extraction Lysis Buffer (with Tris-HCl) is typically used after bacterial culture and cell harvesting.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eCollect bacterial cells by centrifugation after reaching the desired culture density.\u003c\/li\u003e\n\u003cli\u003eAdd appropriate volume of bacterial protein extraction buffer and resuspend the cell pellet thoroughly.\u003c\/li\u003e\n\u003cli\u003eDisrupt bacterial cells using ultrasonic cell disruption under cold conditions to minimize protein degradation.\u003c\/li\u003e\n\u003cli\u003eCentrifuge the lysate to separate soluble protein-containing supernatant and insoluble inclusion body fractions.\u003c\/li\u003e\n\u003cli\u003eUse the collected protein fractions for downstream purification, SDS-PAGE, Western blot, or biochemical analysis.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eOptimization of lysis conditions may be required depending on bacterial strain, expression level, and target protein characteristics.\u003c\/p\u003e\n\u003ch2\u003eOrdering Information\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eAP0991\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eBacterial Protein Extraction Lysis Buffer (with Tris-HCl)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFAQ\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is bacterial protein extraction lysis buffer used for?\u003c\/summary\u003e\n\u003cp\u003eBacterial Protein Extraction Lysis Buffer is used to disrupt bacterial cells and extract intracellular proteins for downstream applications including recombinant protein purification, SDS-PAGE, Western blot, and biochemical analysis.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan this bacterial lysis buffer be used for E. coli protein extraction?\u003c\/summary\u003e\n\u003cp\u003eYes. This bacterial protein extraction buffer is suitable for E. coli expression systems and can be used to prepare bacterial lysates containing soluble proteins and inclusion body fractions.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eDoes this reagent support recombinant protein purification?\u003c\/summary\u003e\n\u003cp\u003eYes. The extracted bacterial protein lysates can be used as starting material for downstream purification workflows, including affinity purification of recombinant proteins.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the purpose of Triton X-100 in bacterial protein extraction buffer?\u003c\/summary\u003e\n\u003cp\u003eTriton X-100 is a nonionic detergent that assists bacterial membrane disruption and improves release of intracellular proteins while maintaining a relatively mild extraction environment.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan this buffer extract inclusion bodies from bacteria?\u003c\/summary\u003e\n\u003cp\u003eYes. After bacterial cell disruption and centrifugation, insoluble fractions containing inclusion bodies can be collected separately for further processing and protein refolding studies.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat downstream applications are compatible with bacterial protein extraction reagent?\u003c\/summary\u003e\n\u003cp\u003eThe extracted bacterial proteins can be used for protein purification, SDS-PAGE analysis, Western blot detection, recombinant protein characterization, and other molecular biology applications.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPRODUCT SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c!-- =========================\nFAQ SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AccuRef Scientific","offers":[{"title":"500mL","offer_id":52776392687797,"sku":"AP0991","price":42.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/5_849f040f-b3dc-41cf-af73-7870899bfb60.jpg?v=1783495653","url":"https:\/\/biofargo.com\/products\/bacterial-protein-extraction-lysis-buffer","provider":"Biofargo","version":"1.0","type":"link"}