{"product_id":"bacterial-protein-extraction-buffer-pbs","title":"Bacterial Protein Extraction Lysis Buffer (with PBS)","description":"\u003c!-- =========================\nPRODUCT DESCRIPTION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eBacterial Protein Extraction Lysis Buffer (with PBS) is a PBS-based bacterial protein extraction buffer designed for efficient disruption of bacterial cells and preparation of protein lysates from expression systems such as \u003ci\u003eEscherichia coli\u003c\/i\u003e. During recombinant protein production, incomplete bacterial cell lysis, unstable extraction conditions, or poor separation of soluble proteins and inclusion bodies can reduce protein recovery and affect downstream purification and analysis. This bacterial cell lysis buffer provides a stable phosphate-buffered environment to help researchers obtain reliable bacterial protein extracts.\u003c\/p\u003e\n\u003cp\u003eFormulated with PBS (phosphate buffered saline), this bacterial protein extraction reagent maintains a suitable ionic environment and pH stability during bacterial protein preparation. After cell disruption, the lysate can be separated into soluble protein fractions and insoluble inclusion body fractions, supporting recombinant protein purification workflows, SDS-PAGE analysis, and protein expression studies.\u003c\/p\u003e\n\u003cp\u003eBacterial Protein Extraction Lysis Buffer (with PBS) is suitable for routine bacterial protein extraction applications, especially for researchers working with \u003ci\u003eE. coli\u003c\/i\u003e expression systems who require a convenient lysis solution for soluble protein preparation and inclusion body recovery.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nSPECIFICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eBacterial Protein Extraction Lysis Buffer (with PBS)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eAP0821\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eVolume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003ePBS-Based Bacterial Protein Extraction Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eBuffer System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003ePBS (Phosphate Buffered Saline)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eBacterial protein extraction, E. coli lysis, recombinant protein preparation, protein purification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSample Types\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eE. coli and other bacterial cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eExtraction Output\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eSoluble protein fraction and inclusion body fraction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eRoom temperature\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eShelf Life\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFEATURES\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003ePBS-based extraction environment:\u003c\/strong\u003e Provides stable phosphate-buffered conditions to support consistent bacterial protein preparation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eEfficient bacterial cell lysis:\u003c\/strong\u003e Designed for disruption of bacterial cells including commonly used \u003ci\u003eE. coli\u003c\/i\u003e expression strains.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSoluble protein and inclusion body separation:\u003c\/strong\u003e Enables fractionation of bacterial lysates into soluble proteins and insoluble inclusion bodies after centrifugation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSupports recombinant protein workflows:\u003c\/strong\u003e Suitable for protein expression analysis and purification preparation from bacterial expression systems.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCompatible with downstream protein analysis:\u003c\/strong\u003e Prepared lysates can be used for SDS-PAGE, Western blot, and biochemical protein studies.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nAPPLICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eBacterial Protein Extraction Lysis Buffer (with PBS) is widely used for bacterial protein preparation, recombinant protein research, and molecular biology workflows requiring efficient bacterial cell disruption.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eE. coli Protein Extraction:\u003c\/strong\u003e Used for extracting recombinant proteins expressed in \u003ci\u003eE. coli\u003c\/i\u003e and other bacterial expression hosts.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBacterial Cell Lysis:\u003c\/strong\u003e Provides a convenient bacterial lysis buffer for releasing intracellular proteins from bacterial cells.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Protein Preparation:\u003c\/strong\u003e Supports preparation of bacterial lysates before affinity purification workflows such as His-tag or GST-tag protein purification.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSoluble Protein Extraction:\u003c\/strong\u003e Helps researchers obtain soluble protein fractions for downstream biochemical analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eInclusion Body Preparation:\u003c\/strong\u003e Enables separation and collection of insoluble inclusion body fractions from overexpressed bacterial proteins.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSDS-PAGE Protein Analysis:\u003c\/strong\u003e Provides bacterial protein samples for electrophoretic analysis and expression verification.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eWestern Blot Detection:\u003c\/strong\u003e Suitable for antibody-based detection of bacterial expressed proteins and recombinant tags.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProtein Purification Research:\u003c\/strong\u003e Used as an initial extraction step before bacterial protein purification and characterization.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPROTOCOL OVERVIEW\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eProtocol Overview\u003c\/h2\u003e\n\u003cp\u003eBacterial Protein Extraction Lysis Buffer (with PBS) is commonly used with mechanical disruption methods such as sonication for efficient bacterial protein extraction.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eCollect bacterial cells by centrifugation after cultivation.\u003c\/li\u003e\n\u003cli\u003eAdd appropriate volume of bacterial protein extraction buffer and resuspend the cell pellet completely.\u003c\/li\u003e\n\u003cli\u003ePerform cell disruption on ice using sonication or other suitable mechanical methods.\u003c\/li\u003e\n\u003cli\u003eCentrifuge the lysate to separate soluble protein fractions and inclusion body fractions.\u003c\/li\u003e\n\u003cli\u003eCollect the supernatant or pellet according to downstream protein purification requirements.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eKeeping samples cold during bacterial protein extraction is recommended to minimize protein degradation and maintain sample quality.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nORDERING INFORMATION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eOrdering Information\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eProduct\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eAP0821\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eBacterial Protein Extraction Lysis Buffer (with PBS)\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFAQ\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is Bacterial Protein Extraction Lysis Buffer (with PBS) used for?\u003c\/summary\u003e\n\u003cp\u003eBacterial Protein Extraction Lysis Buffer (with PBS) is used for bacterial cell lysis and protein extraction, especially for preparing protein samples from \u003ci\u003eE. coli\u003c\/i\u003e expression systems. It helps obtain soluble proteins and inclusion body fractions for downstream purification and analysis.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan this bacterial protein extraction buffer be used for E. coli recombinant protein purification?\u003c\/summary\u003e\n\u003cp\u003eYes. This PBS-based bacterial protein extraction buffer is suitable as an initial extraction step before recombinant protein purification workflows, including preparation of lysates for affinity purification methods.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the difference between soluble proteins and inclusion bodies after bacterial lysis?\u003c\/summary\u003e\n\u003cp\u003eAfter bacterial cell disruption and centrifugation, soluble proteins remain in the supernatant, while insoluble inclusion bodies are collected in the pellet fraction. Separating these fractions helps researchers select appropriate purification strategies.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eDoes this buffer work for bacterial protein extraction from E. coli?\u003c\/summary\u003e\n\u003cp\u003eYes. The buffer is designed for bacterial protein extraction from commonly used expression hosts such as \u003ci\u003eE. coli\u003c\/i\u003e, supporting recombinant protein preparation and protein expression analysis.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat downstream applications are compatible with bacterial lysates?\u003c\/summary\u003e\n\u003cp\u003eBacterial lysates prepared with this buffer can be used for SDS-PAGE, Western blot analysis, protein purification preparation, and biochemical characterization workflows.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhy is PBS used in bacterial protein extraction buffers?\u003c\/summary\u003e\n\u003cp\u003ePBS provides a stable phosphate-buffered environment and maintains suitable ionic conditions during bacterial protein preparation, helping improve extraction consistency.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPRODUCT SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c!-- =========================\nFAQ SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AccuRef Scientific","offers":[{"title":"500mL","offer_id":52776416444597,"sku":"AP0821","price":42.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/5_e0e26342-d590-4e52-8b25-ac415b811315.jpg?v=1783496146","url":"https:\/\/biofargo.com\/products\/bacterial-protein-extraction-buffer-pbs","provider":"Biofargo","version":"1.0","type":"link"}