Description

CRISPR (clustered regularly interspaced short palindromic repeats) and their CRISPR-associated (Cas) proteins constitute the adaptive immune system in bacteria (1-2). This system has been leveraged to create an exemplary genome editing tool with applications in both basic research and therapeutics development (3). AsCas12a is a Cas endonuclease from Acidaminococcus sp. Engineered AsCas12a systems can be used for accurate editing in hematopoietic stem cells, induced pluripotent stem (iPS) cells, and natural killer (NK) cells (2-4).

Accession Number

U2UMQ6

Formulation

25mM Tris, 300mM NaCl, pH7.5, 0.1mM TCEP, 50% glycerol.

Storage

Store product at â€“70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Documents

AsCas12a, Active

References

Horvath, P., et al. 2010. CRISPR/Cas, the immune system of bacteria and archaea. Science, 327(5962):167-170.
Morange, M. 2015. What history tells us XXXVII. CRISPR-Cas: The disconvery of an immune system in prokaryotes. J. Biosci. 40(2):221-223.
Gier, R.A., et al. 2020. High-performance CRISPR-Cas12a genome editing for combinatorial genetic screening. Nat. Commun. 11(1):1-9.
DeLong, R. 2021. Ushering in a new era of RNA-based therapies. Commun Biol. 4(1):557.

Activity

Sample Activity Plot. For specific information on a given lot, see related technical data sheet