Description

Antibody Dilution is an efficient diluent containing various antibody protective agents. The dilution buffer has the properties of enhancing antibody activity and stability, reducing non-specific binding, and improving the specificity and sensitivity of experiments.

Features

Suitable for Western blot, Immunofluorescence and Immunohistochemistry
Enhanced Antibody Stability And Efficiency:
Antibodies diluted with this antibody diluent can be stored at 4°C for up to 6 months without the need for repeated freeze-thaw cycles.
Highly Versatile:
No cross-reactivity with antibodies or target proteins, higher signal-noise ratio compared to non-fat milk and BSA. Suitable for the detection for phosphoproteins.

Fig1. Accelerated stability testing of the primary antibody and HRP-conjugated secondary antibody after dilution with antibody diluent (YamayBio Cat.NO.: BM3153). a. Rabbit Anti-GAPDH antibody and b. Goat anti-Rabbit IgG (H + L), HRP-conjugated antibodies were diluted 1:5000 with antibody diluent. The diluted antibodies were then aliquoted into two tubes and stored at 4°C and 37°C for 14 days, respectively. Subsequently, Western Blot analysis was performed. As shown in the figures, the results indicated that the WB bands obtained with the GAPDH antibody and the HRP-conjugated secondary antibody stored at 37°C for 14 days were comparable to those stored at 4°C for 14 days.

Fig2. Immunofluorescence staining for PSD95 was performed on mouse brain tissue sections using an anti-PSD95 primary antibody diluted 1:100 in antibody diluent (YamayBio Cat. No.:BM3153). A goat anti-rabbit secondary antibody (green fluorescence) was diluted 1:1000 in the same diluent.

Fig3. Formalin-fixed, paraffin-embedded mouse uterine tissue sections were undergo immunohistochemical staining for ER alpha. Anti-ER alpha antibody was diluted in antibody diluent (YamayBio Cat. No.:BM3153) prior to incubation with the tissue sections.

Specifications

Product Type	Buffer
Form	Liquid
Quantity	100 mL / 500 mL

Storage

Transported at room temperature and stored at 4°C. The shelf life is 12 months.

Documents

BM3153-100 COA

BM3153-500 COA

SDS

User Guide

Flyer of Product_ Antibody Diluent

Antibody Diluent-Yamaybio