Description

CD52 is a widely distributed antigen belonging to a GPI anchored glycoprotein with only twelve amino acid residues (GQNDTSQTSSPS). CD52 can be found on the surface of lymphocytes, monocytes, eosophils and dendritic cells in hematopoietic system. with a density of up to 5×10⁵molecules on lymphocytes, CD52 antigens express on many lymphoid cell malignancies and certain acute myeloid leukemia cells. When CD52 molecules are crosslinked with mAb, it triggers a series of signaling within the target cells. The signaling caused by the activation of CD52 molecules is similar to that caused by anti-CD3 antibodies. When CD52 molecules are triggered, PMA (Phorbol Myristate acetate, acetylmyridrobol ester) can promote the proliferation and secretion of cytokines in normal human CD4⁺and CD8⁺Tlymphocytes in vitro.

Characterization

Cytokines
Expression Host	HEK293 cells
QC Testing Purity	> 90 % as determined by SDS-PAGE.
Endotoxin	< 0.1EU per μg of the protein as determined by the LAL method.
Activity	The binding rate with MC/CAR
Formulation	Supplied as a 0.22μm filtered solution in PBS, PH 7.4.
Stability	24 months at 2℃ to 8℃. Avoid repeated freeze-thaw cycles.
Storage	24 months at 2℃ to 8℃. Avoid repeated freeze-thaw cycles.

Documents

GMP-TL112

GMP-TL112_SDS

Anti-Human CD52 mAb T cell activation and expansion