Product Description

Polyclonal sheep IgG antibody raised against E.coli host cell proteins (HCPs) from BL21 produced by physical disruption. Supplied as an affinity-purified, unconjugated liquid antibody in PBS without preservatives. Intended for research applications including HCP coverage evaluations, Western blot and ELISA. Supplied as 1 mg of antibody. Maintain at 2-8°C for short-term use; store below -65°C for long-term storage. Validity period 12 months.

Technical Specifications

Parameter	Details
Size	1 mg
Species Reactivity	Escherichia coli (E. coli)
Host/Isotype	Sheep / IgG
Class	Polyclonal
Type	Antibody
Conjugate	Unconjugate
Immunogen	E.coli HCPs (BL21) produced by physical disruption
Form	Liquid
Purification	Affinity Purified
Storage buffer	PBS
Contains	No preservatives
Applications	HCP Coverage evaluations, Western Blot, ELISA
Storage	Maintain at 2-8°C for short-term use; for long-term storage, store below -65°C
Validity period	12 months

Features

Broad HCP recognition: Polyclonal antibody generated against whole-cell E.coli BL21 HCP mixture to provide broad host cell protein coverage.
Affinity purified: Affinity purification improves specificity and reduces background in immunoassays.
Applications ready: Validated for HCP coverage evaluation workflows and compatible with Western blot and ELISA formats.
Preservative-free formulation: Supplied in PBS without preservatives for compatibility with downstream assays and conjugation.

Applications

HCP coverage evaluations
Western Blot (WB)
Enzyme-linked immunosorbent assay (ELISA)
Research use in process development and assay characterization involving E. coli-expressed proteins

Kit Contents

Anti-E.coli (Protein Expression Strains) HCP Antibody1 mg; store 2-8 °C for short-term; store < -65 °C for long-term

Storage buffer: PBSStore 2-8 °C

* Total: Not applicable (antibody reagent) | Method: ELISA, Western Blot, HCP coverage evaluation

Attention

• For research use only (not intended for diagnostic purposes).

• Maintain at 2-8°C for short-term use; for long-term storage, store below -65°C.

• Avoid repeated freeze–thaw cycles; aliquot as required.

• Verify performance and optimal dilution in your assay system prior to critical experiments.

• Do not use beyond the stated validity period (12 months).

Quality Management & Certifications

Quality System

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QMS (ISO, GMP)

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Quality Advantages

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Quality Control Process

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📂 Technical Resources & Downloads

PDF Product Manual ↓ PDF MSDS / SDS ↓

Frequently Asked Questions (FAQ)

Q1: How should I store this antibody?

Maintain at 2-8°C for short-term use. For long-term storage, store below -65°C. Avoid repeated freeze–thaw cycles by aliquoting upon receipt.

Q2: What applications is this antibody suitable for?

This antibody is intended for HCP coverage evaluations and is compatible with Western blot and ELISA formats. Users should optimize assay conditions (dilution, blocking, incubation times) for their specific workflow.

Q3: Is the antibody conjugated?

No. The product is supplied unconjugated (native IgG). It can be conjugated by the user to labels (HRP, biotin, fluorophores) if required.

Q4: Can this product be used for diagnostic purposes?

No. This product is for research use only (RUO) and is not intended for diagnostic or therapeutic applications.

Research Use Only

✔

Research Use Only (RUO) — Not for diagnostic or therapeutic use. – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.

✔

Not intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.

Anti-E.coli (Protein Expression Strains) HCP Antibody SHENTEK®