Description

Annexin V-EGFP apoptosis detection kit is a kind of apoptosis detection kit, which uses recombinant human Annexin V labeled with EGFP to detect phosphatidylserine appearing on the cell membrane surface during apoptosis. Flow cytometry, fluorescence microscope or other fluorescence detection equipment can be used for detection. Cell apoptosis is one of the most common forms of cell death and is tightly regulated by genes. The process of apoptosis is often accompanied by significant changes in cell morphology. For example, during the early stages of apoptosis, phosphatidylserine (PS), normally located on the inner side of the plasma membrane, flips to the outer surface. In the middle and late stages, as the permeability of the cell membrane increases, larger molecules such as propidium iodide (PI), a DNA-binding dye, can freely diffuse into the cell and stain the nucleus with red fluorescence. Annexin V is a calcium-dependent phospholipid-binding protein with a molecular weight of 35-36 kDa. It specifically binds to phosphatidylserine with high affinity. This assay kit uses Annexin V fused with enhanced green fluorescent protein (EGFP) as a probe to detect phosphatidylserine. In combination with propidium iodide (PI), it enables the rapid detection of cell apoptosis using flow cytometry, fluorescence microscopy, or other fluorescence-based detection methods. After staining with Annexin V-EGFP apoptosis detection reagent, cells undergoing early apoptosis display green fluorescence, while cells in late apoptosis or necrosis show both red and green fluorescence. Live cells show minimal fluorescence.

Features

Stability:

Available as stand-alone reagents or easy-to-use kits.

Specifications

Detection Method	Fluorometric Method ; Flow cytometry
Sample Type	Cell Samples
Dye Type	EGFP
Kit Contents	Annexin V-EGFP / PI / 10√ó Annexin-binding Buffer
Quantity	20 Assays / 50 Assays / 50 Assays √ó2

Storage

Transported in ice packs, stored at 4‚ÑÉ away from light, shelf life is 12 months.

Documents

SDS

Annexin V-EGFP/PI Apoptosis Detection Kit-Yamaybio