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Annexin V-Alexa Fluor 647/PI Apoptosis Detection Kit is an Annexin Fluor 647 labeled with Annexin V as a probe to detect the occurrence of early apoptosis, which can be detected by flow cytometry or other fluorescence detection equipment.
The detection principle is that in normal living cells, phosphotidylserine (PS) is located on the inner side of the cell membrane, but in early apoptotic cells, PS reverses from the inner side to the surface of the cell membrane and is exposed to the extracellular environment. Annexin V is a Ca2+ -dependent phospholipid binding protein with a molecular weight of 35-36 kD that binds PS with high affinity. Phosphatidylserine can bind to the membrane of early-apoptotic cells by exposing it laterally.
In addition, Propidium Iodide (PI) is provided in this kit to distinguish between surviving early cells and necrotic or late apoptotic cells. PI is a kind of nucleic acid dye, which can not penetrate the intact cell membrane of normal cells or early apoptotic cells, but can penetrate the cell membrane of late apoptotic and necrotic cells and make the cell nucleus red. Therefore, when Annexin V was used in combination with PI, PI was excluded from living cells (Annexin V-/PI-) and early apoptotic cells (Annexin V+/PI-). The apoptotic and necrotic cells were double positive by Alexa Fluor 647 and PI binding staining (Annexin V+/PI+).This kit requires flow cytometry.
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