Description

Animal Total RNA Isolation kit uses the spin column and formula developed by our company, which can extract high-purity and high-quality total RNA from various animal tissues with high efficiency. It provides an efficient DNA-Cleaning Column, which can easily separate and adsorb genomic DNA from the supernatant and tissue lysate, making the process simple and time-saving; the RNA-only Column can efficiently bind RNA and can be processed simultaneously with a unique formula suitable for lots of samples.

The whole system is RNase-Free, so that the extracted RNA is not degraded; Buffer RW1, Buffer RW2 buffer washing system, so that the obtained RNA is free of protein, DNA, ion, and organic compound pollution.

Features

No need to worry about RNA degradation; the whole system is RNase-Free
Effectively remove DNA using DNA-Cleaning Column
Remove DNA without adding DNase
Simple—all operations are completed at room temperature
Fast—operation can be completed in 30 minutes
Safe-no organic reagent extraction required
High purity -OD260/280≈1.8-2.1

Application

It is suitable for the extraction and purification of total RNA from a variety of fresh or frozen animal tissues or cultured cells.

Parameters

Downstream applications: first-strand cDNA synthesis, RT-PCR, molecular cloning, Northern Blot, etc.
Samples: animal tissues, cultured cells
Dosage: Tissues 10-20mg, Cells(2-5)×106
Maximum DNA binding capacity of purification column: 80 μg
Elution volume: 50-200 μl

Work Flow

Diagram

Storage

The kit can be stored for 24 months at room temperature (15–25 ℃) and for a longer time at 2–8 ℃. Buffer RL1 can be stored at 4 ℃ for 1 month after adding β- mercaptoethanol (optional).

Documents

Animal Total RNA Isolation Kit Instruction Manual-V1.0-EN