Descprition

This kit uses a DNA-only column that can specifically bind DNA, Foregene protease and a unique buffer system. High-quality genomic DNA can be extracted from various cultured cells and animal tissues within 30 to 50 minutes.

The DNA-only silica gel membrane used in the spin column is Foregene's unique new material, which can effectively and specifically bind to DNA, and maximize the removal of RNA, impurity proteins, ions and other organic compounds in cells. 5-80μg high-quality genomic DNA can be purified from 10-50mg tissue.

Features

No RNase contamination: The DNA-Only Column provided in the kit makes it possible to remove RNA from genomic DNA without additional RNase during the experiment, thereby preventing the laboratory from being contaminated by exogenous RNase.
Fast speed: Foregene Protease has higher activity than similar proteases and digests tissue samples faster;
Simple: the genomic DNA purification operation can be completed in 50 minutes.
Convenient: The centrifugation is performed at room temperature, 4℃ centrifugation and ethanol precipitation of DNA is not required.
Safety: no organic reagent is used.
High quality: The purified genomic DNA has large fragments, no RNA, no RNase, and extremely low ion content, which can meet the requirements of various experiments.

Applications

The genomic DNA purified by Animal Tissue DNA Isolation Kit has high purity and can be used for routine molecular biology operations, such as: enzyme digestion, PCR, Southern hybridization, library construction and other experiments.

Genomic DNA extraction yield and purity

The extraction yield of animal tissue genomic DNA is related to the tissue source, storage conditions, storage time, dosage and other factors. The purity of the obtained genomic DNA meets the routine molecular biology experimental operation, and its OD260/280 is between 1.7-1.9. The yield of genomic DNA extracted using this kit is shown in the following table:

Sample source	Sample amount	DNA yield (μg)	OD260/280
Cell	10⁶	15-30	1.7-1.9
Liver	10mg	4-10	1.7-1.9
Heart	10mg	2-5	1.7-1.9
Spleen	10mg	5-30	1.7-1.9
Brain	10mg	5-10	1.7-1.9

Precautions:

Sample should avoid repeated freezing and thawing, otherwise the extracted DNA fragments will be smaller and the extraction yield will be reduced.
Before using the kit, carefully check whether there is precipitation in Buffer L1, Buffer L2 and Buffer PW. If there is precipitation, please dissolve it at 37℃, mix well before use.
Before using the kit, be sure to check whether Buffer WB is added with anhydrous ethanol according to the instructions. Buffer WB was added with 60ml anhydrous ethanol (DE-05011), 120ml anhydrous ethanol (DE-05012) and 300ml anhydrous ethanol (DE-053013) before use.
Elution volume: Buffer EB should not be less than 100μl, otherwise it will affect the DNA yield.
Remember not to add RNase to any Buffer.
All centrifugation steps are centrifuged at room temperature (15-25℃) using a desktop centrifuge.
All experimental steps were carried out at room temperature (15-25℃).

Storage

The kit can be stored for 12 months at room temperature (15-25℃), or 2-8℃ for longer time.

Note: If stored at low temperature, the solution is prone to precipitation. Before use, be sure to place the solution in the kit at room temperature for a period of time. If necessary, preheat it in a 37℃ water bath for 10 minutes to dissolve the precipitate, and mix it before use.
Foregene Protease solution has a unique formula and is active for a long time (3 months) at room temperature; its activity and stability will be better when stored at 4℃, so it is recommended to store it at 4℃, remember not to store it at -20℃ save.

Workflow

Documents

Animal Tissue DNA Isolation Kit Instruction Manual