Specification

Full name	AMV Reverse Transcriptase
Applications	1. First-strand cDNA synthesis for RT-PCR and real-time RT-PCR; 2. It can increase the reaction temperature and reduce the influence of secondary structure on reverse transcription; 3. Synthesis of cDNA for cloning and expression research; 4. Synthetic labeled cDNA probe; 5. Primer extension method for RNA analysis.
Summary	This product is a recombinant avian myeloblastoma reverse transcriptase expressed by Escherichia coli. AMV RT is a heterodimer consisting of two distinct subunits (alpha 65 KDa and beta 94 KDa subunits). AMV RT has a variety of activities, including RNA and DNA-dependent DNA polymerase activity, DNA-RNA unwinding activity, sequence-specific Mn2+-dependent endonuclease activity, and RNase H (45~60°C) retention activity, so it can reverse transcribe RNA containing a large number of secondary complex structures.
Features	The product uses all imported raw materials, the properties are more stable, the active temperature is 37~58 °C, the reverse transcription temperature can reach 60 °C, and the full-length first-strand cDNA can be efficiently prepared, up to 13kb.
Composition	AMV RT (10 U/ul) and 5X AMV RT Buffer
Reaction Buffer	5X AMV RT buffer
Unit Definition	One unit is defined as the amount ofenzyme required to catalyze the transfer of 1 nmol of Deoxynucleotidein to acid-precipitable material in 10 minutes at 37°C.
Thermal Inactivation	Heat at 85 °C for 5 min to lose activity.
Concentration	10 U/μl
Storage Buffer	200 mM potassium phosphate (pH 7.2 ,4°C), 2 mM DTT, 0.2% (V/V) Triton X-100 and 50% (V/V) glycerol.
Applications	1. First-strand cDNA synthesis for RT-PCR and real-time RT-PCR; 2. It can increase the reaction temperature and reduce the influence of secondary structure on reverse transcription; 3. Synthesis of cDNA for cloning and expression research; 4. Synthetic labeled cDNA probe; 5. Primer extension method for RNA analysis.
Component	AMV RT (10 U/ul) and 5X AMV RT Buffer

Storage

-20°C

AMV Reverse Transcriptase-KleanAB