Description

AMMS® HEK293 serum-free medium is a serum-free, protein-free, and animal-free chemical component qualified medium used in the HEK293 cell suspension culture and transfection process.

The product contains HEPES, sodium bicarbonate, amino acids, vitamins, phenol red, trace elements, F-68, and other components conducive to the growth and expression of HEK293 cells.

L-glutamide should be added before use.

Specifications

Parameter	Specification	Results
Appearance	Red liquid	Compliance
Visible Particle	Should be no visible particle	Compliance
Content	Should not be lower than the indicated value	Compliance
pH	7.0 ~ 8.0	7.2
Endotoxin	< 5 EU/μg	< 0.25 EU/μg
Mycoplasma	Should be negative	Negative
Osmotic Pressure	260 ~ 320 mOsm/kg	272 mOsm/kg
Sterility	No growth	No growth
Cell Proliferation	Cell proliferation rate qualified	Compliance

Feature

✔ Serum-Free, Protein-Free Cell Culture Medium
Chemically defined formulation free of serum, proteins, and animal-derived components, providing a controlled culture environment for HEK293 cells.
✔ Optimized for HEK293 Suspension Culture
Designed to support efficient suspension growth and expression of HEK293 cells during culture and transfection processes.
✔ Comprehensive Nutrient Composition
Contains HEPES, sodium bicarbonate, amino acids, vitamins, phenol red, trace elements, and F-68 to promote robust cell growth and protein expression.
✔ Strict Quality Control and Consistency
Manufactured with rigorous quality control to ensure stable performance, sterility, and reproducible cell proliferation results.

Application

HEK293 Suspension Cell Culture

Suitable for routine cultivation and expansion of HEK293 suspension cells in research laboratories.

Transient Transfection Processes

Supports HEK293-based transfection workflows commonly used for recombinant protein and viral vector production.

Serum-Free Cell Culture Systems

Provides a defined serum-free environment to reduce variability in cell culture experiments.

Biopharmaceutical Research Platforms

Applicable for upstream research workflows involving HEK293 cell-based expression systems.

Stroage

2-8°C, protect from light

Documents

📄 AS-11 Data Sheet 📄 AS-11 SDS 📄 AS-11 COA

AMMS®HEK293 serum-free medium