Description

Alcian Blue 8GX, also known as Ingrain Blue 1, is a basic copper phthalocyanine dye whose copper content imparts a blue color. It is tetravalent, cationic, and water-soluble in nature. It forms salt linkages with the acid groups of acid mucopolysaccharides.

Specifications

Grade	High Purity
Formula	C56H68N16S4Cl14Cu
MW	1298.88
CAS	33864-99-2
Appearance	Dark-blue powder
Identification	PASS
Solubility	75mg dissolved in 250ml de-ionized water yield a clear, bright solution
Dye Content	≥50%
Absorption Max (Spectroscopy)	Report
Storage	18~25℃. Protect from moisture

Application

Alcian Blue 8GX has been used for the staining and precipitation of glycosaminoglycans and as a heteroglycan stain for neutral, sulfated, and phosphated mucopolysaccharides and glycosaminoglycans in tissues such as cartilage and extracellular matrices.
It has been employed in the in vivo visualization of intravascular Bonghan ducts and corpuscles in murine models.
It has been used to develop a novel histochemical staining method to assess multiple respiratory parameters in various species.

Features

Desired staining patterns can be obtained by manipulating pH and salt concentrations.

Principle

The Alcian blue staining is easily combined with the Periodic acid-Schiff (PAS) reaction. In the tissue, acid mucosubstances are stained selectively and nuclear fast red is commonly used as counterstain. In combination with the PAS reaction, non-substituted polysaccharides, neutral mucopolysaccharides, muco- and glycoproteins, and glycol- and phospholipids can be visualized in addition. An Acetic acid-Alcian blue solution is used for the staining, whereby carboxylated and sulfatic proteoglycans are selectively stained when a pH of 2.5 is applied. The differentiation between carboxyl and sulfate groups is only possible at pH 1.

Documents

COA

SDS

Disclaimer: For laboratory research use only.