{"product_id":"aged-mouse-pancreatic-fibroblasts","title":"Aged Mouse Pancreatic Fibroblasts","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eAged Mouse Primary Pancreatic Fibroblasts from Cell Biologics are isolated from the pancreatic tissues of pathogen-free laboratory mice of 58-78 weeks and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Aged Mouse Primary Pancreatic Fibroblasts are characterized by their spindle morphology and immunofluorescence staining with anti-FSP1\/S100A4 (Millipore USA) antibody. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eM2267 Complete Fibroblast Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/A57-6201_A57-6201.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eA57-6201\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/A57-6201_Cell-Culture-Protocol-CB.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eCell-Culture-Protocol-CB\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\n","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":52481614381237,"sku":"A57-6201","price":510.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":52481614414005,"sku":"A57-6201-1","price":560.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":52481614446773,"sku":"A57-6201-2","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Fb1-500x500_3cb14e09-0280-4af0-bc6a-1e2092f0e3f6.jpg?v=1778252712","url":"https:\/\/biofargo.com\/products\/aged-mouse-pancreatic-fibroblasts","provider":"Biofargo","version":"1.0","type":"link"}