{"product_id":"aged-mouse-oral-mucosal-epithelial-cells","title":"Aged Mouse Oral Mucosal Epithelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eAged Mouse Oral Mucosal Epithelial Cells are isolated from oral mucosa tissue of pathogen-free laboratory mice of 58-78 weeks. Aged Mouse Oral Mucosal Epithelial Cells are grown in a T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics\u003c\/i\u003e’ Culture Complete Growth Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with EpCAM-1 (CD326) antibody, followed by the application of magnetic beads pre-coated with secondary antibody.\u003cb\u003e \u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003eAged Mouse Oral Mucosal Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin (Catalog 610182, BD) or ZO-1 (Catalog 617300, Life Technology). Aged Mouse Oral Mucosal Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics\u003c\/i\u003e. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003e\u003cb\u003eCell Biologics\u003c\/b\u003e\u003c\/em\u003e\u003cstrong\u003e' Cell Genotype Promise\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eCell Biologics\u003c\/em\u003e has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). \u003cem\u003eCell Biologics' \u003c\/em\u003ecell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that Cell Biologics' cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project. \u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003e\u003c\/b\u003e  Epithelial Cell Medium \/w Kit – 500 ml\u003c\/p\u003e\n\u003cp\u003eCatalog No. \u003cb\u003eM6621\u003c\/b\u003e\u003c\/p\u003e\n\u003ch2\u003eLaboratory Applications\u003c\/h2\u003e\n\u003cp\u003eAged Mouse Oral Mucosal Epithelial Cells can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/A57-6234_A57-6234.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eA57-6234\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/A57-6234_Cell-Culture-Protocol-CB.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eCell-Culture-Protocol-CB\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eAlthough Aged Mouse Oral Mucosal Epithelial Cells are isolated from laboratory mice testing pathogen-free, investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\n","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":52481618673845,"sku":"A57-6234","price":620.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":52481618706613,"sku":"A57-6234-1","price":670.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":52481618739381,"sku":"A57-6234-2","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Image_1990_ECAD-500x500_7c5e9471-0b00-44a2-aa3b-029b2c267ed4.jpg?v=1778252439","url":"https:\/\/biofargo.com\/products\/aged-mouse-oral-mucosal-epithelial-cells","provider":"Biofargo","version":"1.0","type":"link"}