{"product_id":"aged-mouse-aortic-fibroblasts","title":"Aged Mouse Aortic Fibroblasts","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eAged Mouse Primary Aortic Fibroblasts from Cell Biologics are isolated from the aorta tissues of pathogen-free laboratory mice of 58-78 weeks and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Aged Mouse Primary Aortic Fibroblasts are characterized by their spindle morphology and immunofluorescence staining with anti-FSP1\/S100A4 (Millipore USA) antibody. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eM2267 Complete Fibroblast Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/A57-6075_A57-6075.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eA57-6075\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/A57-6075_Cell-Culture-Protocol-CB.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eCell-Culture-Protocol-CB\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\n","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":52481367408821,"sku":"A57-6075","price":510.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":52481367441589,"sku":"A57-6075-1","price":560.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":52481367474357,"sku":"A57-6075-2","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Fb1-500x500_97b40cd1-0318-4d75-821b-1e0245b65fd5.jpg?v=1778252799","url":"https:\/\/biofargo.com\/products\/aged-mouse-aortic-fibroblasts","provider":"Biofargo","version":"1.0","type":"link"}