Overview

The AdvSHENTEK Mycoplasma DetectInnova System transformed RMM(rapid microbiological method) into a streamlined, fast, fully automated, and easily portable laboratory system. It integrates nucleic acid extraction and real-time PCR assay, allowing a turn-around time of 2.5 hours from sample to result. Importantly it only requires less than 5 minutes of hands-on time, without specific DNA extraction and PCR expertise to operate.

Features

Easy on-time testing

Complete automation from sample to result
Hands-on time<5 min

High Sensitivity & Efficiency

LOD<10 CFU/mL
4 standardized assays in a single run

Accessible Anywhere & Anyone

No PCR lab or skills required
Seamless integration from extraction to amplification without manual intervention

Data Reliability

Fully validated workflow
Effective internal control across every stage
21 CFR Part 11 compliance

High Specificity

No cross-reactivity with diferent bacteria or cell lines
No sample matrix interference

Comprehensive coverage

Able to detect over 180 species of Mycoplasma, Spiroplasma and Achole-plasma

On-time Testing in 3 Easy Steps to Anywhere & Anyone

Table 1DetectInnova Detection Level at 10 & 100 CFU/mL For Different Mycoplasma Species

Strain	Detection Concentration
Strain	10 CFU/mL	100 CFU/mL
Mycoplasma synoviae	24/24	24/24
Mycoplasma arginine	24/24	24/24
Mycoplasma gallisepticam	24/24	24/24
Spiroplasma citri	24/24	24/24
*Mycoplasma orale	24/24	24/24
Mycoplasma hyorhinis	24/24	24/24

Note:24/24=24 out of 24 test results positive

Strain	Detection Concentration
Strain	10 CFU/mL	100 CFU/mL
*Acholeplasma laidlawii	24/24	24/24
*Mycoplasmafermentans	24/24	24/24
*Mycoplasma hominis	24/24	24/24
*Mycoplasma pneumoniae	24/24	24/24
*Mycoplasma salivarium	24/24	24/24

*Strains sourced from ATCC

Table 2 DetectInnova LOD at 10 CFU/mLin Different Matrices

Sample Matrix		Strain
		Mycoplasma orale		Mycoplasma hyorhinis		Mycoplasma pneumoniae
		10 CFU	100 CFU	10 CFU	100 CFU	10 CFU	100 CFU
5% human albumin		24/24	24/24	24/24	24/24	24/24	24/24
CHO-S	10⁶ cells/mL	24/24	24/24	24/24	24/24	24/24	24/24
CHO-S	10⁷cells/mL	24/24	24/24	24/24	24/24	24/24	24/24
DMEM (20%FBS, 10%DMSO)		24/24	24/24	24/24	24/24	24/24	24/24
ExpiCHOTM(10%DMSO)		24/24	24/24	24/24	24/24	24/24	24/24

Note:24/24=24 out of 24 test results positive

Documents

AdvSHENTEK Mycoplasma DetectInnova System_Flyer

AdvSHENTEK Mycoplasma DetectInnova System-SHENTEK™