Description

In biological experiments, it is an important pH stabilizing reagent. Usually, an appropriate weak acid is mixed with its conjugate base to obtain a suitable pH value. Most biological reactions occur under neutral conditions, and the pH is generally selected between 6-8, while the effective buffering range of the buffer is also required to be between 6-8. In addition, it is required that the acidic and basic forms of the buffer do not chelate with certain metal ions. It can also be used as a desulfurizer for water gas and semi-water gas in synthetic ammonia production, as well as a dye intermediate, pharmaceutical intermediate, diagnostic reagent, etc.

Specifications

Item	Value
Chemical Name	N-(2-acetamido)iminodiacetic Acid
Appearance	White Powder
CAS No.	26239-55-4
Molecular Formula	C8H10N2O5
Molecular Weight	190.2
Grade	Biochemical Grade
Purity	≥99%
pH of 1% Aqueous Solution	4.6-6.0 (25℃)
Ignition Residue	≤0.1%
Water Solubility	Transparent, Colorless
Heavy Metals	≤3ppm
Moisture	≤0.5%
Storage / Handling	Store at Room Temperature.

Features

High-purity biochemical-grade ADA (≥98.5%) supplied as a white, free-flowing powder.
Zwitterionic buffering agent with a pKa of approximately 6.6, providing stable pH control in the range of pH 6.0–7.2.
Excellent compatibility with biological and biochemical systems; minimal interference with metal-ion–dependent reactions due to low chelation tendency.
Highly soluble when neutralized, producing clear and colorless solutions suitable for sensitive assays.
Low impurity profile, including moisture ≤0.5%, heavy metals ≤3 ppm, and ignition residue ≤0.1%, ensuring consistent performance in analytical and research applications.

Applications

Biochemical and Molecular Biology Research: Ideal for preparing physiological buffers for enzyme assays, protein studies, nucleic acid workflows, and cell-based experiments requiring near-neutral pH.
Protein Crystallization & Isoelectric Focusing: Frequently used in crystallization buffers and in systems requiring stable and narrow pH ranges.
Analytical Chemistry: Suitable for metal-ion studies, titration systems, and analytical methods requiring low metal chelation.
Diagnostic Reagent & Chemical Synthesis: Serves as an intermediate in dye manufacturing, diagnostic formulation, and other chemical synthesis processes.
General Laboratory Buffer Preparation: Reliable pH stabilizer for routine laboratory solutions across biological, biochemical, and environmental testing workflows.

Safety Disclaimer

This product is intended for laboratory research use only. Not for drug, human, or veterinary use. Review the Safety Data Sheet (SDS) provided by BioFargo for detailed handling, storage, hazard, and disposal information. Use appropriate personal protective equipment (PPE) including gloves, lab coat, and eye protection when handling. Dispose of waste and residual buffer in accordance with local, state, and federal regulations. Ensure proper ventilation if preparing large volumes or heating solutions.

Documents

COA

SDS

ADA Buffer 99% Biochemical Grade