Description

Acridine orange is a metachromatic fluorescent cationic dye that permeates the cell membrane and intercalates DNA and RNA. It allows for visual detection of nucleic acids on agarose and polyacrylamide gels.

Specifications

Grade	Indicator
Formula	C17H20ClN3 · HCl · 1/2ZnCl2
MW	369.96
CAS	10127-02-3
Appearance	Reddish-Brown powder
Solubility/Clarity	Complies
Loss on drying @105 °C/1hr.	Report
Spectro Photometric Assay	Conc. 10mg/l in acetic acid and water Mixture (20:80) at 492.5 nm
Storage	R.T

Application

Acridine Orange, a cell-permeable metachromatic fluorescent cationic dye that intercalates DNA and RNA, is used in fluorescence and epiflouresence microscopy. Acridine Orange dye has been used to analyze mitochondria and lysosomal content by flow cytometry, characterize multidrug resistance, and measure changes in mitochondrial mass during apoptosis in rat thymocytes.

Suitable for

detection of nucleic acids separated by gel electrophoresis
fluorescence and epifluorescence microscopy
analysis of mitochondria and lysosomes by flow cytometry
DNA staining in apoptosis studies

Features

120 microM of acridine orange detects 25-50 ng of purified DNA per band in gels
differential staining of single- and double-stranded polynucleotides

Principle

Acridine orange intercalates into the nucleic acids of double helix and is detectable as green fluorescence at 530 nm. It binds electrostatically to the phosphate groups in single stranded nucleic acids and is detectable at red fluorescence at 640 nm.

Documents

COA

SDS

Disclaimer: For laboratory research use only.

Acridine orange hemi (zinc chloride) salt