Description

The 96 Well PCR Plate Sealing Film is designed to deliver reliable sealing performance for PCR and qPCR applications, helping protect samples from evaporation, contamination, and volume loss during thermal cycling. Manufactured from optically transparent film with a strong, uniform adhesive, it ensures consistent sealing across standard 96-well PCR plates without interfering with fluorescence signal detection.

This DNase/RNase-free PCR sealing film provides excellent adhesion across a wide temperature range and can be easily removed without residue. It is compatible with both manual handling and automated workflows, making it ideal for routine PCR, real-time PCR, and high-throughput molecular biology applications.

Specifications

Parameter	Description
Product Type	PCR Plate Sealing Film
Plate Format	96-Well
Material	Optical transparent film
Adhesive	Pressure-sensitive, residue-free
Cleanliness	DNase/RNase free
Compatibility	PCR & qPCR instruments
Packaging	100 sheets per pack

Operation Principles

Step1

Paste the sealing plate film on the board, remove the single sealing plate film from the packing bag, and then re-seal the packing bag to maintain the enzyme-free environment, keep the backing face up, hold the sealing plate film, and slowly tear off the backing along the tangent line.
Step2

Use the press plate to slowly scrape the seal plate film so that it is fully sealed to the plate. The film pressing step should be performed at least twice horizontally and vertically. Applying enough force (but not too much) is crucial to getting a good seal.
Step3

After sealing, check the plate carefully to make sure that the film is tightly attached to the plate. Verify the paste marks around each hole, that the entire surface of the plate (including the perimeter) is sealed, and that there is no liquid on the film. The sealing plate film should not be wrinkled, and if wrinkles are observed, the plate is not properly sealed.

Features

Designed for 96-Well PCR Plates

Precisely sized to fit standard 96-well PCR plates, ensuring uniform sealing and consistent performance.
Low Evaporation & Secure Seal

Effectively minimizes sample evaporation during thermal cycling and prevents cross-contamination between wells.
Optical Transparency for qPCR

High optical clarity supports accurate fluorescence signal detection in real-time PCR (qPCR) assays.
Strong Adhesive, Residue-Free Removal

Provides a secure seal during PCR while allowing clean removal without adhesive residue.
Wide Temperature Compatibility

Maintains sealing integrity throughout repeated heating and cooling cycles.
DNase/RNase Free & PCR-Grade

Manufactured under clean conditions for sensitive molecular biology workflows.
Automation Compatible

Suitable for manual application as well as automated plate handling systems.

Types of Sealing Films

Clear Polyester Sealing Film:

Characteristics: High performance for robotic applications, excellent seal tominimize evaporation and well-to-well cross-contamination.

Applications: ldeal for extreme conditions and robotic setups.
Adhesive PCR Sealing Film:

Characteristics: Transparent, thermostable, and functional from -70'C to +95'C;reduces cross-contamination in PCR applications.

Applications:Widely used to prevent cross-contamination in PCR experiments
Specialized PCR Plate Sealing Films:

Characteristics: Designed for real-time PCR with ultra-clear properties for opticalanalysis during reactions.

Applications: Suited for real-time PCR experiments requiring optical analysis
Hotseal Sterile Sealing Film:

Characteristics: Suitable for PCR applications, free of RNase and DNase; providesa reliable seal.

Applications: Used in PCR experiments to maintain sterility and integrity.

Applications

PCR experiment:

The sealing membrane used for PCR reaction can prevent cross-infection between PCR plate wells and ensure the accuracy and reliability of experimental results
Biotechnology research and development:

widely used in life sciences and biotechnology research and development, for gene sequencing, DNA amplification and other fields to provide reliable closed performance.
Deep well plate application:

Some sealing membranes are suitable for deep well plates, which can play a protective role in nucleic acid extraction and other experiments to prevent liquid separation and premature wear of magnetic rods
Real-time PCR and quantitative PCR:

For the special needs of real-time PCR and quantitative PCR, some sealing membrane designs have ultra-clear characteristics to support optical analysis
Disease detection:

In the government procurement of CDC, hospitals and other institutions, sealing film is widely used in disease detection and medical research and development.

Advantage

Optical pressure sensitive film: transparent polypropylene film and transparent silicon-based pressure sensitive adhesive bonding, suitable for fluorescence quantitative PCR detection, with high optical clarity, no auto fluorescence
Strong breathable sealing plate membrane: it has strong permeability and is identified as free from human DNA, DNase, RNase and PCR inhibitor contamination. it is suitable for enzyme labeling plates with various hole numbers, shaking bacteria plates, etc.
Meet a variety of applications: can be used for 96-well enzyme plate, 96-well PCR amplification reaction, PCR fluorescence quantification and other application scenarios

Why Choose This PCR Plate Sealing Film

Reliable PCR results depend on consistent sealing. This 96 Well PCR Plate Sealing Film is engineered to reduce evaporation, protect sample integrity, and support accurate fluorescence detection—making it a dependable choice for both routine PCR and demanding qPCR applications. Its balance of performance, ease of use, and compatibility makes it well suited for research, academic, and industrial laboratories.

FAQ

Is this sealing film compatible with real-time PCR (qPCR)?

Yes. This 96 well PCR plate sealing film is optically transparent and suitable for real-time PCR (qPCR) applications. It allows accurate fluorescence signal detection without interfering with optical readings.
Does this PCR sealing film prevent sample evaporation during thermal cycling?

Yes. The film forms a secure seal that significantly reduces evaporation during repeated heating and cooling cycles, helping maintain consistent reaction volumes and reliable PCR results.
Is this PCR plate sealing film DNase and RNase free?

Yes. This DNase/RNase-free PCR sealing film is manufactured under controlled conditions, making it safe for sensitive molecular biology applications.
Can the sealing film be removed without leaving residue?

Yes. The pressure-sensitive adhesive provides strong adhesion during PCR while allowing clean, residue-free removal after amplification.
What plate formats is this sealing film designed for?

This product is specifically designed for standard 96-well PCR plates and ensures uniform sealing across all wells.
Is this PCR sealing film compatible with automated workflows?

Yes. The sealing film is compatible with both manual application and automated plate handling systems, making it suitable for high-throughput PCR workflows.
What temperature range can this sealing film withstand?

The film maintains sealing integrity across a wide temperature range typically used in PCR and qPCR thermal cycling, without warping or loss of adhesion.
How many sealing films are included in each package?

Each package contains 100 sheets of 96 well PCR plate sealing film, suitable for routine laboratory use.
Can this sealing film be used for sample storage?

Yes. In addition to PCR applications, the sealing film can be used for short-term sample protection during storage or transport to help prevent contamination and evaporation.
What makes this PCR plate sealing film a reliable choice?

This PCR plate sealing membrane combines strong adhesion, low evaporation, optical transparency, and clean removal—providing consistent performance for both PCR and qPCR applications.

96 Well PCR Plate Sealing Film, DNase/RNase Free, Optical Transparency, 100 Sheets