Description

PCR sealing membrane is a key tool for PCR (polymerase chain reaction) experiments. Its main uses include: avoiding cross-contamination, preventing evaporation, temperature stability and real-time PCR applications.

The transparent adhesive film firmly seals the sample in the wells, reducing sample leakage, evaporation, and contamination between wells.

The adhesive film is firmly adhered to the well plate by removing the adhesive film backing and grasping the sides to align and secure the adhesive film to the well plate. Once sealed, it can be safely stacked without worrying about contamination.

Specifications

P/N	802021B03
Product name	Product name
Description	Optical, Easily simply, Pressure sensitive
Configuration	100 pcs/pack
Material	Polypropylene (PP)
Color	Transparent
Certificate	ISO9001
Application	Laboratory Usage

Operation Principles

Step1

Paste the sealing plate film on the board, remove the single sealing plate film from the packing bag, and then re-seal the packing bag to maintain the enzyme-free environment, keep the backing face up, hold the sealing plate film, and slowly tear off the backing along the tangent line.
Step2

Use the press plate to slowly scrape the seal plate film so that it is fully sealed to the plate. The film pressing step should be performed at least twice horizontally and vertically. Applying enough force (but not too much) is crucial to getting a good seal.
Step3

After sealing, check the plate carefully to make sure that the film is tightly attached to the plate. Verify the paste marks around each hole, that the entire surface of the plate (including the perimeter) is sealed, and that there is no liquid on the film. The sealing plate film should not be wrinkled, and if wrinkles are observed, the plate is not properly sealed.

Features

Self-adhesive, manual lamination
Both sides can be peeled, easy to operate
Easy to remove, no residue
No reaction with experimental samples
High transparency and good sealing

PCR Plate Sealing Films come in various types, each with distinct characteristicstailored for specific

Types of Sealing Films

Clear Polyester Sealing Film:

Characteristics: High performance for robotic applications, excellent seal tominimize evaporation and well-to-well cross-contamination.

Applications: ldeal for extreme conditions and robotic setups.
Adhesive PCR Sealing Film:

Characteristics: Transparent, thermostable, and functional from -70'C to +95'C;reduces cross-contamination in PCR applications.

Applications:Widely used to prevent cross-contamination in PCR experiments
Specialized PCR Plate Sealing Films:

Characteristics: Designed for real-time PCR with ultra-clear properties for opticalanalysis during reactions.

Applications: Suited for real-time PCR experiments requiring optical analysis
Hotseal Sterile Sealing Film:

Characteristics: Suitable for PCR applications, free of RNase and DNase; providesa reliable seal.

Applications: Used in PCR experiments to maintain sterility and integrity.

Applications

PCR experiment:

The sealing membrane used for PCR reaction can prevent cross-infection between PCR plate wells and ensure the accuracy and reliability of experimental results
Biotechnology research and development:

widely used in life sciences and biotechnology research and development, for gene sequencing, DNA amplification and other fields to provide reliable closed performance.
Deep well plate application:

Some sealing membranes are suitable for deep well plates, which can play a protective role in nucleic acid extraction and other experiments to prevent liquid separation and premature wear of magnetic rods
Real-time PCR and quantitative PCR:

For the special needs of real-time PCR and quantitative PCR, some sealing membrane designs have ultra-clear characteristics to support optical analysis
Disease detection:

In the government procurement of CDC, hospitals and other institutions, sealing film is widely used in disease detection and medical research and development.

Advantage

Optical pressure sensitive film: transparent polypropylene film and transparent silicon-based pressure sensitive adhesive bonding, suitable for fluorescence quantitative PCR detection, with high optical clarity, no auto fluorescence
Strong breathable sealing plate membrane: it has strong permeability and is identified as free from human DNA, DNase, RNase and PCR inhibitor contamination. it is suitable for enzyme labeling plates with various hole numbers, shaking bacteria plates, etc.
Meet a variety of applications: can be used for 96-well enzyme plate, 96-well PCR amplification reaction, PCR fluorescence quantification and other application scenarios

96 Well PCR Plate Sealing Film