Description

Traditional SDS-PAGE gel preparation requires multiple manual steps, including gel percentage calculation, separate preparation of stacking and resolving gels, polymerization reagent handling, and repeated optimization between experiments. These procedures can increase preparation time and introduce variation in gel quality, especially when laboratories require consistent protein separation results for Western blotting and protein characterization.

The 8% One-Step SDS-PAGE Color Gel Fast Preparation Kit (RP0231) is a complete SDS-PAGE gel preparation kit designed for rapid preparation of medium-percentage polyacrylamide gels used in protein electrophoresis applications. The kit contains pre-optimized separation gel solution, concentrating gel solution, corresponding buffers, and a novel coagulant system, enabling researchers to prepare PAGE gels without additional TEMED addition or complex reagent calculations.

Designed for the Tris-glycine SDS-PAGE system, the RP0231 One-Step PAGE Gel Kit provides a simplified gel casting workflow for laboratories analyzing medium to high molecular weight proteins. The 8% polyacrylamide gel concentration offers an optimized pore structure for resolving proteins in the approximately 50–200 kDa range, providing improved separation performance compared with lower percentage gels when analyzing moderately sized proteins.

The colored concentrating gel included in this protein gel casting kit helps visualize loading wells and improves sample loading accuracy. By combining pre-mixed gel preparation reagents with a TEMED-free polymerization workflow, the kit reduces preparation complexity and improves consistency compared with conventional hand-cast SDS-PAGE gels.

The RP0231 kit is suitable for research workflows including Western blot gel preparation, recombinant protein analysis, protein expression studies, and polyacrylamide gel electrophoresis. It provides a practical alternative for laboratories seeking a faster and more reproducible SDS-PAGE gel preparation solution.

Specification

Product Name	8% One-Step SDS-PAGE Color Gel Fast Preparation Kit
SKU	RP0231
Gel Type	Polyacrylamide SDS-PAGE Gel
Gel Concentration	8%
Electrophoresis System	Tris-Glycine SDS-PAGE System
Recommended Protein Range	Approximately 50–200 kDa proteins
Application	Protein electrophoresis, Western blotting, protein separation
Gel Preparation Method	One-step gel casting workflow
Polymerization Time	Approximately 15 minutes
Storage Condition	4 °C; Novel Coagulant long-term storage at −20 °C
Shelf Life	18 months
Research Use	For Research Use Only (RUO)

Kit Components

Component	Volume
Concentrating Gel Solution	80 mL
Concentrating Gel Buffer	80 mL
Separation Gel Solution	250 mL
Separation Gel Buffer	250 mL
Novel Coagulant	8 mL

Features

Optimized 8% SDS-PAGE gel formulation: Provides suitable pore size and separation performance for medium to high molecular weight protein analysis.
One-step SDS-PAGE gel preparation: Pre-optimized gel solutions simplify polyacrylamide gel casting by reducing manual calculations and preparation steps.
TEMED-free PAGE gel preparation: The optimized coagulant system replaces traditional TEMED-assisted workflows, reducing additional reagent handling requirements.
Rapid SDS-PAGE gel preparation: Gel polymerization can be completed in approximately 15 minutes, improving laboratory workflow efficiency.
Color-coded concentrating gel: The visible stacking gel indicator improves identification of loading wells and supports accurate sample application.
Reliable protein separation: The pre-formulated system helps improve batch-to-batch consistency compared with manually prepared SDS-PAGE gels.

Applications

The 8% One-Step SDS-PAGE Color Gel Fast Preparation Kit is designed for researchers requiring reproducible separation of medium to high molecular weight proteins using polyacrylamide gel electrophoresis.

Western blot analysis: Preparation of SDS-PAGE gels for protein transfer and antibody-based detection workflows.
Medium and high molecular weight protein separation: Suitable for proteins requiring an 8% polyacrylamide gel matrix for improved migration and resolution.
Recombinant protein characterization: Analysis of recombinant proteins, purified proteins, and protein expression products.
Protein expression analysis: Evaluation of protein expression levels from bacterial, mammalian, or other biological expression systems.
Protein electrophoresis workflows: Routine SDS-PAGE applications in biotechnology and molecular biology laboratories.
Research and teaching laboratories: Simplified PAGE gel preparation for laboratories requiring efficient and consistent electrophoresis workflows.

Choosing the Right SDS-PAGE Gel Percentage

Selecting the appropriate SDS-PAGE gel concentration is essential for achieving optimal protein separation. Lower percentage polyacrylamide gels provide larger pore sizes for large proteins, while higher percentage gels improve resolution for smaller molecular weight proteins. The 8% SDS-PAGE gel provides a balanced separation range for medium to high molecular weight proteins and is commonly used in protein electrophoresis and Western blot workflows.

Gel Concentration	Recommended Application	Typical Protein Range
6% SDS-PAGE Gel	High molecular weight proteins and large protein complexes	>100 kDa proteins
8% SDS-PAGE Gel	Medium to high molecular weight protein separation	Approximately 50–200 kDa proteins
10% SDS-PAGE Gel	General protein electrophoresis and routine Western blot workflows	Approximately 20–150 kDa proteins
12% SDS-PAGE Gel	Routine protein separation with improved resolution for smaller proteins	Approximately 10–100 kDa proteins
15% SDS-PAGE Gel	Small protein, peptide, and low molecular weight protein analysis	<50 kDa proteins

Preparation Workflow

The RP0231 One-Step SDS-PAGE Gel Preparation Kit simplifies traditional PAGE gel casting by combining optimized gel solutions with a rapid polymerization system. The workflow reduces preparation steps while maintaining compatibility with standard Tris-glycine SDS-PAGE electrophoresis systems.

Mix equal volumes of Separation Gel Solution and Separation Gel Buffer to prepare the resolving gel solution.
Add the Novel Coagulant to the separation gel mixture and gently mix to initiate polymerization while avoiding bubble formation.
Transfer the prepared separation gel solution into the gel cassette.
Mix Concentrating Gel Solution and Concentrating Gel Buffer in equal volumes to prepare the stacking gel solution.
Add Novel Coagulant to the concentrating gel mixture and directly load it above the separation gel layer without waiting for complete resolving gel polymerization.
Insert the comb and allow the gel to polymerize for approximately 15 minutes before performing SDS-PAGE electrophoresis.

This rapid gel preparation workflow provides a convenient alternative to conventional hand-cast SDS-PAGE gels while reducing preparation variation between experiments.

Technical Notes

The RP0231 kit is formulated for denaturing SDS-PAGE electrophoresis. Both separation gel and concentrating gel buffers contain SDS and are not intended for native PAGE applications.
The prepared concentrating gel provides a loading-friendly colored interface and is designed to simplify sample loading. Its performance is similar to commercial precast gel systems rather than traditional high-concentration stacking gels.
Gel polymerization speed may vary depending on ambient temperature. Higher temperatures can accelerate polymerization, while lower temperatures may require additional polymerization time.
For consistent protein separation performance, freshly prepared gels are recommended for electrophoresis experiments.
The 8% polyacrylamide formulation is particularly suitable for samples requiring improved migration of medium to high molecular weight proteins compared with higher percentage SDS-PAGE gels.

Documents

Manual

MSDS

FAQ

What is an 8% SDS-PAGE Gel Preparation Kit used for?

An 8% SDS-PAGE Gel Preparation Kit is designed for preparing polyacrylamide gels suitable for medium to high molecular weight protein separation. The RP0231 kit is commonly used for protein electrophoresis, Western blot sample preparation, recombinant protein analysis, and molecular biology research workflows.

Why choose an 8% SDS-PAGE gel instead of a 6% or 10% gel?

An 8% SDS-PAGE gel provides an intermediate pore size between lower percentage gels designed for very large proteins and higher percentage gels optimized for smaller proteins. It is suitable for researchers who need balanced migration and resolution for proteins approximately 50–200 kDa.

Does RP0231 require TEMED or APS during gel preparation?

No additional TEMED addition is required. The RP0231 kit contains optimized gel preparation reagents and a Novel Coagulant system designed to simplify SDS-PAGE gel casting compared with traditional polymerization workflows.

Can RP0231 be used for Western blot experiments?

Yes. The RP0231 SDS-PAGE protein gel preparation kit is compatible with Western blot workflows where proteins are separated by polyacrylamide gel electrophoresis before membrane transfer and antibody detection.

What protein molecular weight range is recommended for an 8% SDS-PAGE gel?

The RP0231 8% SDS-PAGE gel is recommended for medium to high molecular weight proteins, typically around 50–200 kDa, depending on electrophoresis conditions and sample characteristics.

What are the advantages of a one-step PAGE gel casting kit?

A one-step PAGE gel casting kit reduces preparation steps by combining optimized gel solutions and simplifying polymerization procedures. This helps laboratories improve workflow efficiency and reduce variability caused by manual reagent preparation.

Is RP0231 compatible with standard Tris-glycine SDS-PAGE systems?

Yes. RP0231 is designed for conventional Tris-glycine SDS-PAGE electrophoresis systems and can be used with standard PAGE gel casting and electrophoresis equipment.

What is the difference between RP0231 and other One-Step SDS-PAGE Gel Kits?

The main difference between products in the series is gel concentration. RP0231 provides an 8% gel formulation optimized for medium to high molecular weight protein separation, while other concentrations are designed for different protein size ranges.

8% One-Step PAGE Color Gel Quick Preparation Kit