Description

Traditional SDS-PAGE gel preparation often requires multiple preparation steps, including accurate acrylamide formulation, separate stacking and resolving gel preparation, APS activation, and TEMED addition. These manual steps increase preparation time and introduce variability between experiments, especially when consistent protein separation performance is required for Western blotting and protein characterization workflows.

The 6% One-Step SDS-PAGE Color Gel Fast Preparation Kit (RP0221) is a complete SDS-PAGE gel preparation kit designed for rapid preparation of low-percentage polyacrylamide gels used in protein electrophoresis applications. The kit provides pre-optimized separation gel and concentrating gel solutions together with a novel coagulant system, allowing researchers to prepare PAGE gels without additional TEMED addition or complicated reagent calculations.

Designed for the Tris-glycine SDS-PAGE system, this One-Step PAGE Gel Kit simplifies the gel casting workflow while maintaining reliable protein separation performance. The 6% polyacrylamide gel concentration provides a larger pore size suitable for resolving high molecular weight proteins, protein complexes, and samples requiring improved migration of large proteins.

The prepared concentrating gel contains a visible color indicator, helping researchers easily identify loading wells and improve sample loading accuracy. Compared with conventional hand-cast SDS-PAGE gels, this TEMED-free SDS-PAGE gel preparation solution reduces preparation complexity and improves workflow consistency for laboratories performing routine protein analysis.

The RP0221 kit is intended for research use only and is suitable for applications including Western blot gel preparation, protein expression analysis, recombinant protein characterization, and polyacrylamide gel electrophoresis.

Specification

Product Name	6% One-Step SDS-PAGE Color Gel Fast Preparation Kit
SKU	RP0221
Gel Type	Polyacrylamide SDS-PAGE Gel
Gel Concentration	6%
Electrophoresis System	Tris-Glycine SDS-PAGE System
Application	Protein electrophoresis, Western blotting, protein separation
Gel Preparation Method	One-step gel casting workflow
Polymerization Time	Approximately 15 minutes
Storage Condition	4 °C; Novel Coagulant long-term storage at −20 °C
Shelf Life	18 months
Research Use	For Research Use Only (RUO)

Kit Components

Component	Volume
Concentrating Gel Solution	80 mL
Concentrating Gel Buffer	80 mL
Separation Gel Solution	250 mL
Separation Gel Buffer	250 mL
Novel Coagulant	8 mL

Features

One-step SDS-PAGE gel preparation: Pre-formulated gel solutions eliminate complex concentration calculations and simplify polyacrylamide gel casting.
TEMED-free gel preparation: The optimized coagulant system replaces traditional TEMED-assisted polymerization workflows, reducing handling of additional polymerization reagents.
Rapid PAGE gel preparation: Complete gel polymerization can be achieved in approximately 15 minutes, improving laboratory workflow efficiency.
Color-coded concentrating gel: The colored stacking gel improves visualization of loading wells and supports accurate sample application.
Optimized for high molecular weight proteins: The 6% SDS-PAGE gel provides larger pore sizes suitable for separation of large proteins and protein complexes.
Consistent protein separation: Pre-optimized reagent formulation helps reduce variation caused by manual preparation procedures.

Applications

The 6% One-Step SDS-PAGE Color Gel Fast Preparation Kit is designed for laboratories requiring reliable separation of high molecular weight proteins using polyacrylamide gel electrophoresis.

Western blot analysis: Preparation of SDS-PAGE gels for protein transfer and immunodetection workflows.
High molecular weight protein separation: Suitable for resolving large proteins that require lower percentage polyacrylamide gels.
Protein expression analysis: Evaluation of recombinant protein expression and purification results.
Protein characterization: Analysis of purified proteins, protein complexes, and molecular weight distribution.
Biotechnology research: Routine protein electrophoresis workflows in molecular biology laboratories.
Academic and teaching laboratories: Simplified SDS-PAGE gel preparation for training and educational experiments.

Choosing the Right SDS-PAGE Gel Percentage

The optimal SDS-PAGE gel concentration depends on protein molecular weight and desired separation resolution. Lower percentage polyacrylamide gels contain larger pores and are commonly selected for high molecular weight proteins, while higher percentage gels provide better resolution for smaller proteins.

Gel Concentration	Recommended Application	Typical Protein Range
6% SDS-PAGE Gel	High molecular weight proteins and large protein complexes	>100 kDa proteins
8% SDS-PAGE Gel	Medium to high molecular weight protein separation	Approximately 50–200 kDa proteins
10% SDS-PAGE Gel	General protein electrophoresis and routine Western blot workflows	Approximately 20–150 kDa proteins
12% SDS-PAGE Gel	Routine protein separation with improved resolution for smaller proteins	Approximately 10–100 kDa proteins
15% SDS-PAGE Gel	Small protein, peptide, and low molecular weight protein analysis	<50 kDa proteins

Preparation Workflow

The RP0221 kit provides a simplified protein gel casting workflow compared with conventional SDS-PAGE gel preparation methods.

Mix equal volumes of Separation Gel Solution and Separation Gel Buffer to prepare the resolving gel solution.
Add Novel Coagulant to initiate gel polymerization and gently mix to avoid bubble formation.
Transfer the resolving gel mixture into the gel cassette.
Prepare the concentrating gel solution by mixing Concentrating Gel Solution and Concentrating Gel Buffer.
Add Novel Coagulant and directly cast the concentrating gel without waiting for complete separation gel polymerization.
Insert the comb and allow approximately 15 minutes for gel polymerization before electrophoresis.

The simplified casting procedure reduces preparation steps while maintaining compatibility with standard Tris-glycine SDS-PAGE electrophoresis workflows.

Technical Notes

The RP0221 kit contains SDS in both separation and concentrating gel buffers and is designed for denaturing SDS-PAGE electrophoresis.
The prepared concentrating gel functions similarly to commercial precast gels and does not provide the same sample stacking effect as traditional manually prepared stacking gels.
Gel polymerization speed is temperature dependent. Higher temperatures may accelerate polymerization, while lower temperatures may require extended polymerization time.
For optimal electrophoresis performance, freshly prepared gels are recommended for use.

Documents

Manual

MSDS

FAQ

What is a One-Step SDS-PAGE Gel Preparation Kit?

A One-Step SDS-PAGE Gel Preparation Kit is a pre-optimized reagent system designed to simplify polyacrylamide gel preparation for protein electrophoresis. Unlike traditional SDS-PAGE gel casting workflows that require separate calculations and addition of multiple polymerization reagents, this kit provides ready-to-use gel solutions and a simplified casting procedure for faster protein gel preparation.

What are the advantages of a TEMED-free SDS-PAGE gel preparation kit?

Traditional SDS-PAGE gel preparation commonly uses TEMED together with APS to initiate acrylamide polymerization. The RP0221 One-Step PAGE Gel Kit uses a novel coagulant system instead of additional TEMED addition, helping simplify gel casting and reduce handling of traditional polymerization reagents.

How long does it take to prepare an SDS-PAGE gel using RP0221?

The RP0221 SDS-PAGE Gel Preparation Kit can complete gel polymerization in approximately 15 minutes under suitable conditions. The one-step workflow eliminates several preparation steps associated with conventional hand-cast PAGE gels.

What proteins are suitable for a 6% SDS-PAGE gel?

A 6% SDS-PAGE gel has larger pore sizes and is recommended for separation of high molecular weight proteins, protein complexes, and samples where improved migration of large proteins is required. It is commonly used for proteins above approximately 100 kDa.

Can the RP0221 PAGE gel kit be used for Western blot?

Yes. The RP0221 SDS-PAGE protein gel preparation kit is suitable for Western blot workflows requiring separation of proteins by polyacrylamide gel electrophoresis before transfer to membranes for antibody-based detection.

Is RP0221 compatible with standard SDS-PAGE electrophoresis systems?

Yes. The kit is designed for Tris-glycine SDS-PAGE systems and can be used with standard PAGE electrophoresis equipment used in molecular biology and protein analysis laboratories.

What is the difference between a 6% SDS-PAGE gel and a 10% or 12% SDS-PAGE gel?

The main difference is pore size. A 6% SDS-PAGE gel provides larger pores suitable for high molecular weight proteins, while 10% and 12% gels are commonly selected for general protein separation and lower molecular weight proteins.

Does this kit require APS or TEMED during gel preparation?

The RP0221 kit is supplied with optimized gel solutions and a Novel Coagulant system. Additional TEMED is not required during gel preparation.

6% One-Step PAGE Color Gel Quick Preparation Kit