Specification

Full name	6×His Tag rabbit polyclonal antibody
Reactivity	Fusion proteins containing the HIS Tag
Applications	WB
Host	Rabbit
Clone type	rabbit polyclonal
Conjugate	Unconjugate
Target Background	The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification. It can then be purified using immobilized metal ions, traditionally nickel, in a procedure termed immobilized-metal affinity chromatography. Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting. The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells.
WB Predicted band size	55 kDa
WB Positive control	0.5/0.25/0.5/0.25 μg Fusion protein containing the HIS Tag
WB Recommended dilution	2000-10000

Storage

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol