Specification

Full name	6×His tag mouse monoclnal antibody
Alternative names	50 μl/100 μl
Reactivity	mouse monoclonal
Applications	WB, IF, IP
Host	Mouse
Clone type	mouse monoclonal
Target Background	The 6 X histidine motif is used as a Tag on many recombinant proteins to facilitate purification. It can then be purified using immobilized metal ions, traditionally nickel, in a procedure termed immobilized-metal affinity chromatography. Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting. The antibody recognizes the His-Tag fused to targeted proteins in transfected or transformed cells.
Swissprot No
Gene Accession
WB Predicted band size	50 kDa
WB Positive control	Fusion proteins containing the His Tag
WB Recommended dilution	1000-5000
IHC predicted cell location
IHC positive control
IHC Recommed dilution

Storage

Ascitic fluid with 0.05% sodium azide and 40% glycerol

P113832-WB-1-P