Description

Traditional SDS-PAGE gel preparation requires multiple manual steps, including preparation of acrylamide solutions, separate preparation of resolving and stacking gel buffers, adjustment of gel concentration, and addition of polymerization reagents. These procedures can increase preparation time and introduce variation between experiments, especially when laboratories need different gel percentages for proteins with different molecular weights.

The 6–15% SDS-PAGE Gradient Gel Quick-Preparation Kit (AP0641) is a complete SDS-PAGE gel preparation kit designed for preparing polyacrylamide gels with adjustable separation ranges. The kit provides the essential reagents required for SDS-PAGE gel casting, including 30% Acr-Bis (29:1) solution, pre-mixed resolving gel buffer, pre-mixed stacking gel buffer, and a novel polymerization enhancer.

Unlike conventional SDS-PAGE gel preparation workflows that require additional TEMED addition, the AP0641 TEMED-free SDS-PAGE gel preparation kit uses an optimized polymerization enhancer system to simplify gel casting procedures. Researchers only need to provide gel casting equipment and distilled water to prepare SDS-PAGE gels for routine protein electrophoresis applications.

The 6–15% gradient SDS-PAGE gel preparation system allows users to prepare different polyacrylamide gel concentrations according to protein molecular weight requirements. From high molecular weight protein separation using lower percentage gels to improved resolution of smaller proteins using higher percentage gels, AP0641 provides flexibility for different protein electrophoresis workflows.

The stacking gel contains a visible non-migrating dye, allowing researchers to easily identify sample wells during loading. This feature improves loading accuracy and simplifies handling during SDS-PAGE protein electrophoresis workflows. The AP0641 kit is suitable for applications including Western blot gel preparation, protein molecular weight analysis, recombinant protein characterization, and polyacrylamide gel electrophoresis.

The AP0641 SDS-PAGE gel casting kit is supplied for research use only and provides a convenient alternative to conventional manual PAGE gel preparation methods for molecular biology and biotechnology laboratories.

Specification

Product Name	6–15% SDS-PAGE Gradient Gel Quick-Preparation Kit
SKU	AP0641
Product Type	SDS-PAGE Gel Preparation Kit
Gel Type	Polyacrylamide SDS-PAGE Gel
Gel Concentration Range	6–15%
Acrylamide/Bisacrylamide Ratio	29:1
Electrophoresis System	Tris-Glycine SDS-PAGE System
Gel Preparation Method	Manual gel casting with pre-mixed reagents
Polymerization Time	Approximately 15 minutes
Gel Capacity	50 gels
Resolving Gel Buffer	4× buffer containing Tris (pH 8.8) and SDS
Stacking Gel Buffer	4× buffer containing Tris (pH 6.8) and SDS
Storage Condition	4°C; Polymerization enhancer stored at −20°C
Shelf Life	18 months
Research Use	For Research Use Only (RUO)

Kit Components

Component	Volume	Storage
30% Acr-Bis (29:1)	100 mL	4°C, protected from light
Resolving Gel Buffer (4×)	70 mL	4°C
Stacking Gel Buffer (4×)	30 mL	4°C
Novel Polymerization Enhancer	5 mL	−20°C, protected from light

Features

Adjustable 6–15% SDS-PAGE gel preparation: The AP0641 kit enables preparation of different polyacrylamide gel concentrations according to experimental requirements, supporting separation of proteins across a broad molecular weight range.
Complete SDS-PAGE gel casting solution: The kit contains pre-mixed resolving gel buffer and stacking gel buffer with SDS, reducing the number of preparation steps required for conventional PAGE gel casting.
TEMED-free SDS-PAGE workflow: The included polymerization enhancer eliminates the need for additional TEMED addition during gel preparation, simplifying laboratory operation.
Rapid polyacrylamide gel preparation: SDS-PAGE gels can be prepared within approximately 15 minutes, improving efficiency for routine protein electrophoresis workflows.
Visible dye stacking gel: The non-migrating dye in the stacking gel provides clear visualization of sample wells for more accurate protein loading.
Consistent protein separation: Pre-mixed components help reduce variability caused by repeated manual preparation of individual gel reagents.
Suitable for multiple protein analysis workflows: The flexible gel concentration range supports routine SDS-PAGE, Western blot sample separation, and molecular weight analysis.

Applications

The 6–15% SDS-PAGE Gradient Gel Quick-Preparation Kit (AP0641) is designed for laboratories requiring flexible polyacrylamide gel preparation for protein separation and analysis. By allowing researchers to prepare different SDS-PAGE gel concentrations, the kit supports a wide range of protein electrophoresis applications from high molecular weight proteins to smaller protein fragments.

Protein electrophoresis: Suitable for routine SDS-PAGE protein separation workflows requiring reliable polyacrylamide gel preparation and consistent protein band migration.
Western blot sample preparation: Provides SDS-PAGE gels for separating protein samples before transfer to membranes for antibody-based detection workflows.
Protein molecular weight analysis: Different gel percentages allow researchers to optimize separation conditions according to protein molecular weight and sample characteristics.
Recombinant protein analysis: Suitable for evaluating recombinant protein expression, purification results, and molecular weight differences during biotechnology research.
Low molecular weight protein separation: Higher percentage SDS-PAGE gels prepared from AP0641 provide improved resolution for smaller proteins compared with lower percentage polyacrylamide gels.
High molecular weight protein analysis: Lower percentage gels provide larger pore structures suitable for separation of larger proteins and protein complexes.
Protein characterization studies: Applicable for laboratories performing protein quality evaluation, sample comparison, and biochemical analysis.
Molecular biology and biotechnology research: Provides a convenient SDS-PAGE gel casting solution for academic laboratories, biotechnology companies, and research institutions.

Preparation Workflow

The AP0641 SDS-PAGE Gel Preparation Kit provides a simplified workflow for preparing polyacrylamide gels using pre-mixed reagents. The optimized formulation reduces preparation complexity while maintaining compatibility with standard SDS-PAGE electrophoresis systems.

Select the required SDS-PAGE gel concentration according to the molecular weight of the target protein.
Mix distilled water, 30% Acr-Bis (29:1), and the appropriate 4× resolving gel buffer to prepare the resolving gel solution.
Add the polymerization enhancer and mix gently to initiate gel formation.
Cast the resolving gel into the gel cassette and allow polymerization.
Prepare the stacking gel using the 4× stacking gel buffer, Acr-Bis solution, distilled water, and polymerization enhancer.
The visible dye-containing stacking gel allows easy identification of sample wells before protein loading.
Perform SDS-PAGE electrophoresis using either rapid electrophoresis buffer conditions or conventional Tris-glycine-SDS running buffer conditions.

The AP0641 workflow enables laboratories to prepare customized SDS-PAGE gels while reducing manual reagent preparation compared with traditional hand-casting methods.

Technical Notes

The AP0641 kit is designed for denaturing SDS-PAGE electrophoresis workflows. The resolving and stacking gel buffers contain SDS and are intended for protein separation under denaturing conditions.
The kit provides a 30% Acr-Bis (29:1) solution, allowing users to prepare different gel percentages according to experimental requirements.
The stacking gel contains a non-migrating dye that improves visualization of loading wells during sample application.
At lower temperatures, SDS precipitation may occur in gel buffers. Warm the solutions gently and mix thoroughly before use.
Polymerization time depends on environmental conditions. Lower room temperatures may require adjustment of polymerization enhancer usage according to experimental conditions.
The product is supplied for Research Use Only (RUO) and is not intended for diagnostic or therapeutic applications.

FAQ

What is a SDS-PAGE Gel Preparation Kit used for?

A SDS-PAGE Gel Preparation Kit is used to prepare polyacrylamide gels for protein electrophoresis. The AP0641 kit provides Acr-Bis solution, resolving gel buffer, stacking gel buffer, and polymerization enhancer for preparing SDS-PAGE gels with different concentrations.

Does the AP0641 SDS-PAGE gel kit require TEMED?

No. The AP0641 kit uses a novel polymerization enhancer system and does not require additional TEMED addition during SDS-PAGE gel preparation. This simplifies gel casting procedures and reduces exposure to TEMED-related odor.

What gel percentages can be prepared with the AP0641 kit?

The AP0641 gradient SDS-PAGE gel preparation kit supports preparation of 6%, 8%, 10%, 12%, and 15% polyacrylamide gels. The selected gel concentration depends on the molecular weight range and required separation resolution.

What proteins are suitable for 6–15% SDS-PAGE gels?

The AP0641 kit supports protein separation across a broad molecular weight range. Lower percentage gels are suitable for larger proteins, while higher percentage gels provide improved separation of smaller proteins.

Can AP0641 be used for Western blot workflows?

Yes. AP0641 can be used to prepare SDS-PAGE gels for Western blot workflows where protein samples require separation before membrane transfer and antibody detection.

What is the advantage of a colored stacking gel?

The colored stacking gel contains a non-migrating dye that makes sample loading wells easier to identify. This helps improve loading accuracy during SDS-PAGE sample preparation.

What is the difference between resolving gel and stacking gel buffer?

The resolving gel buffer contains Tris at pH 8.8 and SDS and forms the main separation layer of the SDS-PAGE gel. The stacking gel buffer contains Tris at pH 6.8 and SDS and helps concentrate protein samples before separation.

How long does it take to prepare an SDS-PAGE gel using AP0641?

The AP0641 SDS-PAGE gel preparation workflow can produce a gel in approximately 15 minutes, depending on temperature and experimental conditions.

What is the difference between a 6% and 15% SDS-PAGE gel?

A 6% SDS-PAGE gel has larger pores and is suitable for higher molecular weight proteins, while a 15% SDS-PAGE gel provides smaller pores and improved resolution for lower molecular weight proteins.

Is AP0641 suitable for native PAGE?

No. AP0641 is designed for SDS-PAGE workflows and contains SDS in the gel buffer system. It is not intended for native PAGE applications.