{"product_id":"5x-protein-loading-buffer-reducing","title":"5× Protein Loading Buffer (Reducing)","description":"\u003c!-- =========================\nPRODUCT DESCRIPTION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003e5× Protein Loading Buffer (Reducing) is a concentrated SDS-PAGE protein sample loading buffer designed for efficient protein denaturation and reduction before gel electrophoresis. During protein sample preparation, incomplete protein unfolding or insufficient disulfide bond reduction can affect protein migration, molecular weight estimation, and downstream Western blot analysis. This reducing protein loading buffer provides a reliable solution for preparing consistent protein samples by combining strong denaturing conditions with an advanced reducing system.\u003c\/p\u003e\n\u003cp\u003eUnlike traditional SDS-PAGE sample buffers containing DTT or β-mercaptoethanol, this 5× reducing sample buffer uses a new-generation odor-free reducing agent that provides strong reduction capability with improved thermal stability. The optimized formulation maintains reducing performance during high-temperature sample treatment while avoiding the unpleasant odor associated with commonly used reducing reagents.\u003c\/p\u003e\n\u003cp\u003e5× Protein Loading Buffer (Reducing) is suitable for protein electrophoresis workflows including SDS-PAGE, Western blot sample preparation, recombinant protein analysis, and cell or tissue lysate evaluation. The formulation is designed to maintain a neutral pH after heating, helping minimize protein degradation during sample preparation.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nSPECIFICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e5× Protein Loading Buffer (Reducing)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eAP0181\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e5× SDS-PAGE Protein Sample Loading Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e5× Concentrated Reducing Loading Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eVolume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e1 mL × 10\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eReducing System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eOdor-free reducing agent, DTT and β-mercaptoethanol free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSample Ratio\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e4 μL protein sample + 1 μL 5× loading buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProtein Denaturation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e100°C heating for 5–10 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eSDS-PAGE, Western blot, protein electrophoresis, protein sample preparation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e-20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eShelf Life\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFEATURES\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eStrong reducing performance:\u003c\/strong\u003e Provides efficient disulfide bond reduction to promote complete protein denaturation before SDS-PAGE analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eOdor-free reducing formulation:\u003c\/strong\u003e Eliminates the strong odor associated with β-mercaptoethanol and provides a more convenient laboratory workflow.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eHigh thermal stability:\u003c\/strong\u003e Maintains reducing activity during high-temperature protein sample treatment.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCompatible with SDS-PAGE and Western blot workflows:\u003c\/strong\u003e Supports routine protein electrophoresis and immunoblotting applications.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eOptimized sample preparation:\u003c\/strong\u003e Maintains neutral pH after heating to help reduce protein degradation during denaturation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e5× concentrated format:\u003c\/strong\u003e Provides flexible sample preparation with reduced reagent consumption compared with ready-to-use loading buffers.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nAPPLICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003e5× Protein Loading Buffer (Reducing) is widely used for preparing protein samples in molecular biology, biochemistry, and protein analysis workflows.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSDS-PAGE Protein Electrophoresis:\u003c\/strong\u003e Prepares denatured and reduced protein samples for separation according to molecular weight.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eWestern Blot Sample Preparation:\u003c\/strong\u003e Generates consistent protein lysates for electrophoresis followed by antibody-based detection.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCell Lysate Protein Analysis:\u003c\/strong\u003e Suitable for preparing whole-cell protein samples from cultured cells.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eTissue Protein Extraction Analysis:\u003c\/strong\u003e Supports analysis of proteins extracted from biological tissue samples.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Protein Characterization:\u003c\/strong\u003e Used for evaluating recombinant protein expression and purity after purification.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProtein Molecular Weight Determination:\u003c\/strong\u003e Enables accurate comparison of protein migration patterns during SDS-PAGE.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProtein Expression Studies:\u003c\/strong\u003e Suitable for research involving protein abundance comparison and expression analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProteomics Sample Preparation:\u003c\/strong\u003e Provides standardized protein denaturation and reduction before electrophoretic analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPROTOCOL OVERVIEW\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eProtocol Overview\u003c\/h2\u003e\n\u003cp\u003eFor SDS-PAGE sample preparation, combine protein samples with 5× Protein Loading Buffer (Reducing) according to the recommended dilution ratio.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eAdd 1 μL of 5× Protein Loading Buffer to every 4 μL of protein sample.\u003c\/li\u003e\n\u003cli\u003eMix thoroughly to ensure complete sample homogenization.\u003c\/li\u003e\n\u003cli\u003eHeat samples at 100°C for 5–10 minutes to denature proteins.\u003c\/li\u003e\n\u003cli\u003eCool samples to room temperature.\u003c\/li\u003e\n\u003cli\u003eCentrifuge at 10,000–14,000 rpm for 5 minutes.\u003c\/li\u003e\n\u003cli\u003eTransfer the supernatant and load directly onto SDS-PAGE gels.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis product contains denaturing components and is intended for SDS-PAGE workflows. It is not suitable for native PAGE or non-denaturing electrophoresis.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nORDERING INFORMATION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eOrdering Information\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eAP0181\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e5× Protein Loading Buffer (Reducing)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e1 mL × 10\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFAQ\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is 5× Protein Loading Buffer (Reducing) used for?\u003c\/summary\u003e\n\u003cp\u003e5× Protein Loading Buffer (Reducing) is used for SDS-PAGE protein sample preparation. It denatures proteins and reduces disulfide bonds before electrophoresis, helping generate consistent protein migration patterns for protein analysis and Western blot workflows.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the difference between reducing and non-reducing protein loading buffer?\u003c\/summary\u003e\n\u003cp\u003eReducing protein loading buffer contains reducing components that break disulfide bonds within and between proteins. Non-reducing sample buffers do not disrupt these bonds and are used when maintaining disulfide-linked protein structures is required.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan this reducing sample buffer be used for Western blot?\u003c\/summary\u003e\n\u003cp\u003eYes. This SDS-PAGE reducing sample buffer is suitable for preparing protein samples before electrophoresis and Western blot detection.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eDoes this protein loading buffer contain DTT or β-mercaptoethanol?\u003c\/summary\u003e\n\u003cp\u003eNo. This odor-free reducing protein loading buffer does not contain DTT or β-mercaptoethanol. It uses an alternative reducing system designed to provide strong reduction performance with improved laboratory handling.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhy use a 5× protein loading buffer instead of a 2× sample buffer?\u003c\/summary\u003e\n\u003cp\u003eA 5× concentrated loading buffer allows flexible sample preparation with a smaller buffer volume while maintaining effective protein denaturation and reduction conditions.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan this buffer be used for native PAGE?\u003c\/summary\u003e\n\u003cp\u003eNo. Because this product contains denaturing components, it is designed for SDS-PAGE and is not suitable for native PAGE or non-denaturing electrophoresis.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow should 5× Protein Loading Buffer (Reducing) be stored?\u003c\/summary\u003e\n\u003cp\u003eThe product should be stored at -20°C. Under recommended storage conditions, the shelf life is 24 months.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPRODUCT SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c!-- =========================\nFAQ SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AccuRef Scientific","offers":[{"title":"1mL×10","offer_id":52778523394229,"sku":"AP0181","price":46.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_53e1848d-a405-4d33-97c9-299c14c352ab.jpg?v=1783565603","url":"https:\/\/biofargo.com\/products\/5x-protein-loading-buffer-reducing","provider":"Biofargo","version":"1.0","type":"link"}