Description

Preparing consistent polyacrylamide gels requires accurate acrylamide and bisacrylamide ratios to control gel structure and electrophoretic separation performance. Variations in acrylamide concentration or crosslinking ratio may affect pore size, protein migration behavior, and reproducibility during PAGE workflows.

30% Acrylamide/Bisacrylamide Solution (37.5:1) is a ready-to-use PAGE gel preparation reagent containing acrylamide and bisacrylamide at a standardized 37.5:1 ratio. This premixed solution helps simplify polyacrylamide gel preparation by reducing preparation steps and providing consistent composition for routine electrophoresis applications.

Designed for molecular biology and biochemical research workflows, this Acrylamide/Bisacrylamide solution is commonly used for SDS-PAGE gel preparation, polyacrylamide gel electrophoresis, protein separation, and other PAGE-based analysis applications.

30% Acrylamide/Bisacrylamide Solution (37.5:1) is part of a PAGE gel preparation reagent series, providing different acrylamide/bisacrylamide ratios to support various electrophoresis requirements.

Specifications

Product Name	30% Acrylamide/Bisacrylamide Solution (37.5:1)
SKU	AP0441L
Volume	500 mL
Product Type	Acrylamide-Bisacrylamide PAGE Gel Preparation Reagent
Acrylamide/Bisacrylamide Ratio	37.5:1
Concentration	30%
Applications	PAGE gel preparation, SDS-PAGE, protein electrophoresis, polyacrylamide gel electrophoresis
Storage	4 °C
Shipping Condition	Room temperature transport
Shelf Life	12 months

Features

Standardized 37.5:1 acrylamide/bisacrylamide ratio: Provides a consistent crosslinking composition for routine PAGE gel preparation.
Ready-to-use 30% Acrylamide/Bisacrylamide solution: Reduces preparation time compared with weighing and mixing individual acrylamide components.
Supports reproducible electrophoresis workflows: Helps maintain consistent gel preparation conditions for protein and nucleic acid separation.
Compatible with common PAGE applications: Suitable for SDS-PAGE reagent preparation, polyacrylamide gel electrophoresis, and protein analysis workflows.
Convenient laboratory storage: Designed for refrigerated storage at 4 °C with a 12-month stability period.

Applications

30% Acrylamide/Bisacrylamide Solution (37.5:1) is widely used as a core reagent for polyacrylamide gel preparation in molecular biology and protein research workflows.

SDS-PAGE Gel Preparation: Used as a stock acrylamide solution for preparing polyacrylamide gels used in protein electrophoresis.
Protein Electrophoresis: Supports separation and analysis of proteins based on molecular size using PAGE workflows.
Western Blot Sample Analysis: Provides the acrylamide component required for preparation of separation gels used before protein transfer and antibody detection.
Polyacrylamide Gel Electrophoresis: Suitable for routine PAGE workflows requiring controlled acrylamide and bisacrylamide composition.
Nucleic Acid Electrophoresis: Can be used for PAGE-based nucleic acid separation applications requiring polyacrylamide gel matrices.
Molecular Biology Research: Used in laboratory workflows involving protein characterization, electrophoretic analysis, and biochemical studies.

Protocol Overview

30% Acrylamide/Bisacrylamide Solution (37.5:1) can be used as a stock reagent for preparing polyacrylamide gels according to the requirements of specific PAGE applications.

Select the appropriate final acrylamide concentration according to the target molecule size and separation requirement.
Combine the Acrylamide/Bisacrylamide solution with other PAGE gel preparation components according to the selected gel formulation.
Allow gel polymerization before performing electrophoresis experiments.
Use prepared polyacrylamide gels for downstream protein or nucleic acid separation workflows.

Ordering Information

SKU	Product	Size
AP0441L	30% Acrylamide/Bisacrylamide Solution (37.5:1)	500 mL

FAQ

What is 30% Acrylamide/Bisacrylamide Solution (37.5:1) used for?

30% Acrylamide/Bisacrylamide Solution (37.5:1) is used for preparing polyacrylamide gels in PAGE workflows, including SDS-PAGE, protein electrophoresis, and other molecular biology applications requiring acrylamide-based gel matrices.

What does the 37.5:1 acrylamide/bisacrylamide ratio mean?

The 37.5:1 ratio represents the relative proportion of acrylamide to bisacrylamide in the solution. This ratio determines the crosslinking characteristics of the resulting polyacrylamide gel and influences gel structure during electrophoresis.

What is the difference between 29:1 and 37.5:1 Acrylamide/Bisacrylamide solutions?

The main difference is the acrylamide-to-bisacrylamide crosslinking ratio. Different ratios create different gel properties and may be selected according to electrophoresis requirements, separation performance, and experimental workflow.

Can this Acrylamide/Bisacrylamide solution be used for SDS-PAGE?

Yes. 30% Acrylamide/Bisacrylamide Solution (37.5:1) is commonly used as a stock reagent for SDS-PAGE gel preparation and protein electrophoresis workflows.

How should 30% Acrylamide/Bisacrylamide Solution be stored?

The solution should be stored at 4 °C. The product is stable for 12 months under recommended storage conditions.

Why use a premixed Acrylamide/Bisacrylamide solution instead of preparing it from individual reagents?

A premixed Acrylamide/Bisacrylamide solution simplifies gel preparation by reducing manual weighing and mixing steps, helping researchers achieve consistent reagent composition during PAGE workflows.

30% Acr-Bis（37.5:1）