Description

Preparing consistent polyacrylamide gels requires accurate acrylamide and bisacrylamide ratios to achieve reliable separation performance during electrophoresis workflows. Variations in gel preparation can affect pore structure, electrophoretic separation quality, and downstream protein or nucleic acid analysis. 30% Acrylamide/Bisacrylamide Solution (29:1) is a ready-to-use PAGE gel preparation reagent designed to simplify polyacrylamide gel preparation for SDS-PAGE and other polyacrylamide gel electrophoresis applications.

30% Acr-Bis (29:1) contains acrylamide and bisacrylamide at a 29:1 ratio in aqueous solution and serves as a commonly used stock solution for preparing polyacrylamide gels. By providing a standardized acrylamide-bisacrylamide mixture, this reagent helps researchers prepare gels for protein electrophoresis, SDS-PAGE analysis, and nucleic acid separation workflows.

This acrylamide/bis-acrylamide solution is suitable for laboratories performing PAGE gel preparation and is commonly used together with other electrophoresis reagents for customized polyacrylamide gel casting according to experimental requirements.

Specifications

Product Name	30% Acrylamide/Bisacrylamide Solution (29:1)
SKU	AP0101L
Volume	500 mL
Product Type	Acrylamide/Bisacrylamide PAGE Gel Preparation Solution
Acrylamide : Bisacrylamide Ratio	29:1
Concentration	30% Acrylamide-Bisacrylamide Solution
Applications	PAGE gel preparation, SDS-PAGE, protein separation, nucleic acid electrophoresis
Storage	4°C, protected from light
Shelf Life	18 months

Features

Standardized acrylamide/bisacrylamide mixture: Provides a consistent 29:1 acrylamide to bisacrylamide ratio for polyacrylamide gel preparation.
Ready-to-use PAGE gel reagent: Eliminates the need for individual weighing and mixing of acrylamide and bisacrylamide components.
Compatible with SDS-PAGE workflows: Suitable for preparing polyacrylamide gels used in protein electrophoresis and protein separation.
Supports customized gel preparation: Allows researchers to prepare PAGE gels according to specific electrophoresis requirements.
Suitable for protein and nucleic acid analysis: Used as a common laboratory stock solution for PAGE-based separation applications.

Applications

30% Acrylamide/Bisacrylamide Solution (29:1) is widely used as a core reagent for polyacrylamide gel electrophoresis workflows in molecular biology and biochemical research.

SDS-PAGE Protein Electrophoresis: Used for preparing polyacrylamide gels for protein separation before staining or Western blot analysis.
PAGE Gel Preparation: Provides the acrylamide and bisacrylamide components required for laboratory-prepared PAGE gels.
Protein Separation Research: Supports electrophoretic separation of proteins according to molecular characteristics.
Nucleic Acid Electrophoresis: Used in polyacrylamide gel systems for separation and analysis of nucleic acid samples.
Western Blot Sample Analysis: Supports SDS-PAGE workflows used for subsequent protein detection and characterization.
Molecular Biology Research: Suitable for laboratories conducting protein analysis, gel electrophoresis, and biochemical studies.

Protocol Overview

30% Acrylamide/Bisacrylamide Solution (29:1) is used as a stock reagent during polyacrylamide gel preparation. Researchers can combine this solution with appropriate gel preparation components according to the required PAGE gel formulation.

Prepare PAGE gel components according to the selected gel concentration and experimental requirements.
Add 30% Acrylamide/Bisacrylamide Solution (29:1) as the acrylamide source during gel preparation.
Mix gel components thoroughly and prepare the polyacrylamide gel according to the selected electrophoresis protocol.
Use the prepared gel for SDS-PAGE, PAGE electrophoresis, protein separation, or nucleic acid analysis.

For SDS-PAGE gel preparation, refer to compatible SDS-PAGE gel preparation protocols and reagents.

Ordering Information

SKU	Product	Size
AP0101L	30% Acrylamide/Bisacrylamide Solution (29:1)	500 mL

FAQ

What is 30% Acrylamide/Bisacrylamide Solution (29:1) used for?

30% Acrylamide/Bisacrylamide Solution (29:1) is used as a stock reagent for preparing polyacrylamide gels. It is commonly used for SDS-PAGE, PAGE gel preparation, protein electrophoresis, and nucleic acid separation workflows.

Why is the acrylamide to bisacrylamide ratio 29:1 important?

The 29:1 ratio provides a standardized acrylamide and bisacrylamide composition commonly used for polyacrylamide gel preparation. The ratio influences the gel structure and separation characteristics during electrophoresis.

Can this product be used for SDS-PAGE gel preparation?

Yes. 30% Acrylamide/Bisacrylamide Solution (29:1) is commonly used for preparing polyacrylamide gels for SDS-PAGE protein electrophoresis workflows.

What types of samples can be separated using PAGE gels prepared with this reagent?

PAGE gels prepared with this reagent can be used for separation and analysis of proteins and nucleic acids depending on the selected electrophoresis system.

How should 30% Acrylamide/Bisacrylamide Solution be stored?

The product should be stored at 4°C and protected from light. The recommended shelf life is 18 months under proper storage conditions.

Is acrylamide solution hazardous?

Acrylamide-containing products require careful handling. Appropriate laboratory protective equipment, including gloves and proper laboratory practices, should be used during operation.

30% Acrylamide-Bisacrylamide (29:1)