Background

The 2X Taq PCR Master Mix (with dye) is a ready-to-use reagent designed for DNA amplification by polymerase chain reaction (PCR). It contains recombinant Taq DNA polymerase derived from Thermus aquaticus and produced in an E. coli expression system. Taq polymerase catalyzes DNA synthesis by incorporating nucleotides onto primer-template complexes. This polymerase exhibits 5'→3' polymerase activity along with weak 5'→3' exonuclease activity and lacks proofreading (3'→5' exonuclease) functionality, leaving adenine overhangs at amplified fragment 3' ends, facilitating subsequent TA cloning assays. The added dye permits direct loading of PCR products onto agarose gels without additional loading buffers. This reagent is suitable for routine molecular biology applications including genotyping, cloning, and DNA sequence analysis, assisting researchers to reduce experimental variability by minimizing handling steps.

Specification

Item	Value
Product Name	2X Taq PCR Master Mix (with Dye)
Package Size	1 mL
Applications	Conventional PCR
Composition	2X SanTaq PCR Mix (with bromophenol blue dye)
Taq Polymerase Source	Recombinant, E. coli Expression
Concentration	25 μg / 500 μL
Color	Blue
Storage	−20 °C

Features

Ready-to-Use 2X Formulation

Pre-mixed with Taq DNA polymerase, MgCl₂, dNTPs, PCR buffer, and enhancers for fast PCR setup.
Direct Gel Loading

Contains bromophenol blue dye, enabling PCR products to be loaded directly onto agarose gels.
Reliable Amplification Performance

Optimized buffer system supports stable and efficient amplification in routine PCR assays.
TA Cloning Compatible

Generates PCR products with 3′-dA overhangs, facilitating efficient ligation into T-vectors.
Enhanced Stability

Formulated with stabilizers; can be stored at 4 °C for up to 3 months and long-term at −20 °C.

Applications

Conventional PCR amplification
Gene cloning and insert preparation
Genotyping and mutation analysis
Template preparation for DNA sequencing
Routine molecular biology research workflows

Storage

Store the 2X master mix at -20°C.

Documents

2X Taq PCR Master Mix (with Dye)