Intended Use

The 2X qPCR Master Mix is used for real-time qualitative and quantitative qPCR with TaqMan probe.
The master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probe and DNA template.

Product Characterizations

The kit is designed for singleplex qPCR with TaqMan probe.
This kit uses Taq-Probe DNA polymerase specially engineered for TaqMan probe, which increased 5’ to 3’ exonuclease efficiency produces S-shaped curve.
The concentrations of the primers and probe are variable depending on specific assays and thermocycling protocols (User Manual Table 1).
The preferable PCR product size is ≤150bp.
The kit has three formulations of ROX, Low ROX or High ROX concentrations for your choice (see User Manual Table 2).