Intended Use

The 2X Multiplex qPCR Master Mix is used for real-time qualitative and quantitative multiplex qPCR with TaqMan probes for up to 4 targets.
The master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probes and templates.

Product Characterizations

The kit is designed for multiplex qPCR with TaqMan probes.
The kit uses Taq-Probe DNA polymerase specially engineered for TaqMan probes, which increases 5’ to 3’ exonuclease efficiency and produces S-shaped curve.
Two to four pairs of gene-specific primers can be applied in one reaction.
The concentrations of the primers and probes are variable depending on specific assays and thermocycling protocols (User Manual Table 1).
The preferable PCR product size is ≤150bp.
The kit has three formulations of ROX, Low ROX or High ROX concentrations for your choice (see User Manual Table 2).