Intended Use

The 2X Fast qPCR Master Mix is used for real-time qualitative and quantitative qPCR with SYBR Green dye.
The master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers and DNA template.

Product Characterizations

The kit is designed for singleplex qPCR with SYBR Green dye.
The kit contains Taq-Fast DNA polymerase which can extend more than 300 bases with a short cycling program.
The concentrations of the primers are variable depending on assay designs and thermo-cycling protocols (User Manual Table 1).
The preferable PCR product size is ≤150bp.
The kit has three formulations of ROX, Low ROX or High ROX concentrations for your choice (see User Manual Table 2).