Description

Biofargo polycarbonate Erlenmeyer flasks are engineered for reliable suspension cell culture applications in biopharmaceutical research, vaccine production, and protein expression.

Each flask is manufactured from optically clear, high-strength polycarbonate (PC) under cGMP-compliant, automated production to ensure consistent quality and sterility. The flasks are gamma-irradiated, nonpyrogenic, and free from detectable cytotoxic substances, making them ideal for sensitive mammalian cell cultures such as CHO, 293, and Sf9 lines.

The hydrophobic vent cap provides efficient gas exchange while preventing contamination, and the precision-molded T-type sealing system ensures a tight, leakproof connection. The flask body features uniform wall thickness, reinforced conical shape, and clear graduations, offering excellent stability and visibility during orbital shaking.

Dimensionally and functionally compatible with standard 250 mL baffled polycarbonate Erlenmeyer shake flasks used in mammalian cell culture. Head-to-head validation data available on request.

250 mL PC Erlenmeyer flask with baffled concave bottom and PTFE-lined screw vent cap

Specification

Cap material	PP
Flask material	PC (polycarbonate)
Cap Style	Vented
Cap color	Green
Neck O.D. (Outer Diameter)	43 mm (approx.)
Flask Style	Erlenmeyer
Top Style	Threaded
Graduation Interval	50ml
Temperature Resistance	121℃
Endotoxin	≤0.05 EU/ml
Vent Filter	0.22 µm hydrophobic membrane
Sterilization	Gamma irradiated, sterile, nonpyrogenic
Packaging	Individually packed; 12 flasks per case (Cat. No. 23205L)

A detailed specification comparison sheet is available for download in the Documents section below.

Features

High-quality polycarbonate (PC) construction – excellent optical clarity (≥80% light transmittance), high mechanical strength, and reliable temperature resistance from −130 °C to +130 °C.
Gamma-irradiated, sterile, and nonpyrogenic – produced under cGMP conditions with no human contact to ensure product consistency and biosafety.
Hydrophobic vent cap membrane – maintains effective gas exchange while preventing liquid leakage or contamination.
Precision-engineered sealing system – integrated T-type leakproof closure with conical threads for smooth tightening and secure fit.
Anti-slip and anti-eccentric neck design – ensures safe handling and prevents accidental slippage during shaking.
Uniform wall thickness and reinforced conical shape – offers superior pressure resistance and durability for high-speed shaker applications.
Concave bottom design – enhances stability and ensures even mixing performance.
Clear volume graduations and writable marking area – easy volume observation and sample identification.
Ideal for suspension cell culture – optimized for CHO, 293, Sf9, and other suspension cell lines used in biopharmaceutical, vaccine, and protein production.

Application

Designed for the cultivation of various suspension cells, including 293 and CHO.
Widely used in process development, biopharmaceutical production, vaccine development, and protein research.
Ideal for small-scale experiments, preliminary studies, media optimization, and pilot screenings of bacterial or mammalian cell cultures.
Compact 250 mL Erlenmeyer Flask ensures efficient gas exchange and nutrient utilization in small-scale laboratory setups.
Suitable for small-scale process development and stepwise scale-up culture stages.

Documents

Product Comparison Sheet (PDF)

FAQ

What is PC?

PC refers to polycarbonate, a high-performance engineering plastic with excellent transparency, impact resistance, and temperature resistance. It is used as the flask material.

Can the flask be autoclaved?

Yes. The flask can be autoclaved at 121°C. One autoclave cycle is recommended.

What is the recommended working volume?

For this 250 mL flask, a working volume of approximately 30%–40% of the total flask capacity is recommended, depending on the cell line, shaking speed, and oxygen-transfer requirements.

Validation Data

Material Extractable

Endotoxin

Dnase / Rnase / Pyrogen Free

Sealing

Torque Performance

Membrane Function

250 mL PC Erlenmeyer Flask Baffled Concave Bottom with PTFE-Lined Screw Vent Cap, Sterile, Autoclavable, Individually Packed