Descprition

This product contains our company's unique Foregene Taq DNA Polymerase, dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer and stabilizer. The unique reaction system and high-efficiency Taq DNA Polymerase make the PCR reaction have higher amplification efficiency, specificity and sensitivity.

During PCR amplification, specific amplified bands can be obtained in 5-10 cycles less than similar products at home and abroad, and the target gene can be detected and cloned from trace and trace samples at a lower number of cycles. The optimized reaction system can greatly reduce human error; it is suitable for the amplification and detection of conventional PCR reactions, complex templates, and trace templates.

Product components

2× PCR EasyTM Mix include:0.1U Foregene Taq DNA Polymerase/μl, 400 μM dNTPs, 20 mM Tris-HCl(pH8.8), 100 mM KCl, 3 mM MgCl2, other stabilizers and enhancers.

Features

The 2× PCR HeroTM Mix system has higher tolerance to PCR inhibitors than the ordinary PCR Mix system, and can easily cope with PCR amplification of various complex templates. The unique reaction system and high-efficiency Taq Hero make the PCR reaction have higher amplification efficiency, specificity and sensitivity.

Kit Application

PCR amplification of DNA fragments
DNA labeling
DNA sequencing
PCR plus A tail

Instructions

PCR EasyTM is convenient and quick to use, and can avoid contamination during PCR operation and experimental errors caused by multiple preparations of the reaction system to the greatest extent. When using, just take half the volume of the reaction system (for example, if the reaction system is 50μl, take 25μl 2× PCR EasyTM Mix solution) 2× PCR EasyTM Mix, add template and primers, and add deionized water to make up the volume to make the reaction system. The reaction can be carried out when the concentration of PCR Mix is 1×.

Practical examples

1.Reaction System

2× PCR EasyTM Mix	10μl
Template DNA	xμl
Primer 1(10μM)	0.4μl
Primer 2(10μM)	0.4μl
ddH2O	Fill to 20μl
Total volume	20µl

2.Reaction conditions

Temperature	Time	Cycles
94°C	3min	1 25-40
94°C	10sec
55°C	20sec
72°C	2kb/min
72°C	5min	1

Note: For 10µl and 20µl systems, if the PCR machine does not have a thermal cover, add an equal volume of mineral oil. The PCR reaction conditions are based on the specific conditions of the template, primer set, and the GC content and length of the primers to design the best reaction conditions, including annealing temperature, extension time, etc.

Storage and Shelf life

It can be stored for a long time at -20°C; for frequent use, it can be stored at 4°C for a short time.

2×High-efficiency PCR reaction premix system PCR EasyTM