2×Hieff Canace Plus PCR Master Mix (With Dye)

2×Hieff Canace Plus PCR Master Mix (With Dye) is a ready-to-use 2×premixed solution containing Hieff Canace Plus High-Fidelity DNA Polymerase, dNTPs, and optimized buffer. Two monoclonal antibodies at room temperature that inhibit polymerase activity and 3'→5' exonuclease activity are added to the master mix for easily and highly specific Hot Start PCR. The extension factor is added to the master mix to give the enzyme a long fragment amplification capacity, the length of the amplification t can be up to 13 kb, the enzyme has a 5'→3' DNA polymerase activity and a 3'→5' exonuclease activity, its fidelity is 83 times that of Taq DNA polymerase, which is 9 times that of ordinary Pfu DNA polymerase. It is suitable for the amplification of complex templates, the amplification product is a blunt end.

2×Hieff Canace Plus PCR Master Mix (With Dye) has the advantages of being fast and easy, high sensitivity, strong specificity, good stability, etc., the reaction system only needs to add primers and templates, and can be amplified by a two-step protocol, simplifying the experimental steps and saving time. This product contains electrophoresis indicator dyes, and PCR products can be used directly for electrophoresis. In addition, the product also contains a specific protective agent, so that the master mix can maintain stable activity after repeated freeze-thaw.