{"product_id":"1x-dsdna-hs-assay-kit","title":"1X dsDNA HS Assay Kit","description":"\u003cstyle\u003e\n  .bf-rich .josh-faq {\n    background: #fff;\n    color: #000;\n    font-family: \"IBM Plex Sans\", sans-serif;\n  }\n\n  .bf-rich .josh-faq__inner { padding: 30px 300px 60px 30px; }\n  .bf-rich .josh-faq__item { border-bottom: 1px solid #e5e7eb; padding: 0; }\n  .bf-rich .josh-faq__item:first-child { border-top: 1px solid #e5e7eb; }\n\n  .bf-rich .josh-faq__question {\n    width: 100%;\n    margin: 0;\n    padding: 20px 0;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    color: #10213d;\n    background: none;\n    border: 0;\n    font: 700 16px\/1.4 \"IBM Plex Sans\", sans-serif;\n    text-align: left;\n    cursor: pointer;\n    list-style: none;\n  }\n\n  .bf-rich .josh-faq__question::-webkit-details-marker { display: none; }\n  .bf-rich .josh-faq__question:hover { color: #055da9; }\n\n  .bf-rich .josh-faq__icon {\n    position: relative;\n    width: 20px;\n    height: 20px;\n    flex: 0 0 20px;\n    transition: transform .25s ease;\n  }\n\n  .bf-rich .josh-faq__icon::before,\n  .bf-rich .josh-faq__icon::after {\n    position: absolute;\n    content: \"\";\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity .25s ease, transform .25s ease;\n  }\n\n  .bf-rich .josh-faq__icon::before {\n    top: 50%;\n    left: 0;\n    width: 100%;\n    height: 2px;\n    transform: translateY(-50%);\n  }\n\n  .bf-rich .josh-faq__icon::after {\n    top: 0;\n    left: 50%;\n    width: 2px;\n    height: 100%;\n    transform: translateX(-50%);\n  }\n\n  .bf-rich .josh-faq__item[open] .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .bf-rich .josh-faq__answer {\n    max-width: 860px;\n    padding: 0 0 20px;\n    color: #333;\n    font: 400 16px\/1.6 \"IBM Plex Sans\", sans-serif;\n  }\n\n  .bf-rich .josh-faq__answer p { margin: 0 0 15px; color: #333; font: inherit; }\n  .bf-rich .josh-faq__answer p:last-child { margin-bottom: 0; }\n  .bf-rich .josh-faq__answer ul { margin: 8px 0 12px; padding-left: 22px; }\n  .bf-rich .josh-faq__answer li { margin-bottom: 6px; font: inherit; list-style-type: disc; }\n\n  @media (max-width: 767px) {\n    .bf-rich .josh-faq__inner { padding: 24px 0 40px; }\n    .bf-rich .josh-faq__question { padding: 16px 0; font-size: 15px; }\n    .bf-rich .josh-faq__answer { font-size: 15px; }\n  }\n\u003c\/style\u003e\n\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eLaboratories measuring low concentrations of double-stranded DNA may find that UV absorbance at 260 nm is affected by other substances in the sample. Preparing a working solution before every assay also adds a handling step.\u003c\/p\u003e\n\u003cp\u003eThe Biofargo 1× dsDNA HS Assay Kit provides a ready-to-use, fluorescence-based method for high-sensitivity dsDNA quantification. The supplied 1× dsDNA HS Working Solution requires no dilution. Add the sample, incubate for 2 minutes at room temperature protected from light, and measure the fluorescence.\u003c\/p\u003e\n\u003cp\u003eThe assay is highly selective for dsDNA over RNA. It accepts 1–20 µL of sample in a final assay volume of 200 µL. The current product details state an initial dsDNA concentration range of 10 pg\/µL–100 ng\/µL, depending on sample volume, and a total assay range of 0.2–100 ng.\u003c\/p\u003e\n\u003cp\u003eThe kit supports Qubit 2, Qubit 3, Qubit 4, and Qubit Flex fluorometers. It can also be used with a fluorescence microplate reader at 485 nm excitation and 530 nm emission. When protected from light, the fluorescent signal remains stable for up to 3 hours after incubation.\u003c\/p\u003e\n\u003cp\u003eFor Research Use Only. Not for use in diagnostic or therapeutic procedures.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 90%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eParameter\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eSpecification\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eProduct Name\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eBiofargo 1× dsDNA HS Assay Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eHigh Sensitivity dsDNA Assay\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eTarget\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eDouble-stranded DNA (dsDNA)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eDetection Method\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eFluorescence\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eFormulation\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eReady-to-use 1× dsDNA HS Working Solution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eInitial Sample Concentration\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e10 pg\/µL–100 ng\/µL, depending on sample volume\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eTotal Assay Range\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e0.2–100 ng\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e1–20 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eFinal Assay Volume\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e200 µL per tube or well\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eAssay Temperature\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eRoom temperature, 20–25°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eIncubation\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e2 minutes at room temperature, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eSignal Stability\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eUp to 3 hours after incubation when protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eCompatible Fluorometers\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eQubit 2, Qubit 3, Qubit 4, and Qubit Flex fluorometers\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eMicroplate Reader Compatibility\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e485 nm excitation and 530 nm emission\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eRecommended Microplate\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eBlack-wall, clear-bottom 96-well microplate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eShipping Condition\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eShip on ice\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e2–8°C, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eIntended Use\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eResearch Use Only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eReady-to-use formulation: The supplied 1× dsDNA HS Working Solution can be used without dilution.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eHigh-sensitivity dsDNA quantification: The current product details state a total assay range of 0.2–100 ng.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSelective dsDNA measurement: The assay is highly selective for double-stranded DNA over RNA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFlexible sample input: Each assay accepts 1–20 µL of sample in a final volume of 200 µL.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eShort incubation: Incubate for 2 minutes at room temperature while protected from light.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eStable reading window: The fluorescent signal remains stable for up to 3 hours when protected from light.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eMultiple instrument formats: The kit supports the specified Qubit fluorometers and a fluorescence microplate reader.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eContaminant tolerance: DNA standards were evaluated with BSA, sodium acetate, ammonium acetate, sodium chloride, magnesium chloride, phenol, chloroform, ethanol, SDS, Triton X-100, ssDNA, polyethylene glycol, RNA, and dNTPs. In the supplied results, “OK” usually represents less than 10% perturbation at the indicated final assay concentration.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eHigh-Sensitivity dsDNA Quantification with a Qubit Fluorometer:\u003c\/strong\u003e Equilibrate the kit components to room temperature. Use thin-wall, clear 0.5 mL PCR tubes and label their lids rather than their sides. Prepare each standard with 190 µL of 1× dsDNA HS Working Solution and 10 µL of QS0 or QS1. Prepare each sample with 180–199 µL of Working Solution and 1–20 µL of sample, for a final volume of 200 µL. Use a 2 µL pipette when adding 1–2 µL of sample. Vortex each tube for 3–5 seconds, then incubate for 2 minutes at room temperature protected from light. On a Qubit 4 Fluorometer, select dsDNA, then 1× dsDNA High Sensitivity. Read QS0 followed by QS1, select the sample volume and output units, and read the samples. Avoid warming the tubes by holding them before measurement. Do not use calibration curves generated with other kits.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eHigh-Sensitivity dsDNA Quantification with a Fluorescence Microplate Reader:\u003c\/strong\u003e Equilibrate the kit components to room temperature and mix thoroughly. Use a black-wall, clear-bottom 96-well microplate. Add 190 µL of Working Solution to each standard well and 180–199 µL to each sample well. Serially dilute QS1 to prepare standards from 0 to 10 ng\/µL, then add 10 µL of each standard to its designated well. Add 1–20 µL of each sample so every well contains exactly 200 µL. Vortex the plate for 10–15 seconds and incubate for 2 minutes at room temperature protected from light. Read at 485 nm excitation and 530 nm emission. Generate a standard curve to calculate unknown concentrations. If a sample exceeds the linear range, dilute it and repeat the assay.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 90%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFS-T1005-S — 100 Reactions\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFS-T1005 — 1,200 Reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1× dsDNA HS Working Solution\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e20 mL\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e250 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003edsDNA HS Standard QS0 (0 ng\/µL)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e100 µL\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003edsDNA HS Standard QS1 (10 ng\/µL)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e100 µL\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eStore at 2–8°C, protected from light.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eShip on ice.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eFrequently Asked Questions (FAQs)\u003c\/h2\u003e\n\u003cdiv class=\"josh-faq\"\u003e\n\u003csection class=\"josh-faq__inner\"\u003e\n\u003cdiv class=\"josh-faq__list\"\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\n\u003csummary class=\"josh-faq__question\"\u003e What is the Biofargo 1× dsDNA HS Assay Kit? \u003cspan class=\"josh-faq__icon\"\u003e\u003c\/span\u003e \u003c\/summary\u003e\n\u003cdiv class=\"josh-faq__answer\"\u003e\n\u003cp\u003eIt is a ready-to-use, high-sensitivity fluorescence assay for quantifying double-stranded DNA. The kit includes a premixed 1× dsDNA HS Working Solution and two dsDNA standards.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\n\u003csummary class=\"josh-faq__question\"\u003e Does the 1× dsDNA HS Working Solution require dilution? \u003cspan class=\"josh-faq__icon\"\u003e\u003c\/span\u003e \u003c\/summary\u003e\n\u003cdiv class=\"josh-faq__answer\"\u003e\n\u003cp\u003eNo. The supplied solution is ready to use and does not require preparation or dilution before adding standards or samples.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\n\u003csummary class=\"josh-faq__question\"\u003e What is the assay range? \u003cspan class=\"josh-faq__icon\"\u003e\u003c\/span\u003e \u003c\/summary\u003e\n\u003cdiv class=\"josh-faq__answer\"\u003e\n\u003cp\u003eThe current product details state a total assay range of 0.2–100 ng and an initial dsDNA concentration range of 10 pg\/µL–100 ng\/µL, depending on sample volume.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\n\u003csummary class=\"josh-faq__question\"\u003e Is the kit compatible with Qubit fluorometers? \u003cspan class=\"josh-faq__icon\"\u003e\u003c\/span\u003e \u003c\/summary\u003e\n\u003cdiv class=\"josh-faq__answer\"\u003e\n\u003cp\u003eYes. The protocol supports Qubit 2, Qubit 3, Qubit 4, and Qubit Flex fluorometers. On a Qubit 4 Fluorometer, select dsDNA and then 1× dsDNA High Sensitivity.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\n\u003csummary class=\"josh-faq__question\"\u003e Can the kit be used with a fluorescence microplate reader? \u003cspan class=\"josh-faq__icon\"\u003e\u003c\/span\u003e \u003c\/summary\u003e\n\u003cdiv class=\"josh-faq__answer\"\u003e\n\u003cp\u003eYes. Use a black-wall, clear-bottom 96-well microplate and set the reader to 485 nm excitation and 530 nm emission.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\n\u003csummary class=\"josh-faq__question\"\u003e How much sample is required for each assay? \u003cspan class=\"josh-faq__icon\"\u003e\u003c\/span\u003e \u003c\/summary\u003e\n\u003cdiv class=\"josh-faq__answer\"\u003e\n\u003cp\u003eUse 1–20 µL of sample in a final assay volume of 200 µL. When adding 1–2 µL of sample, use a 2 µL pipette.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\n\u003csummary class=\"josh-faq__question\"\u003e How long should the assay be incubated? \u003cspan class=\"josh-faq__icon\"\u003e\u003c\/span\u003e \u003c\/summary\u003e\n\u003cdiv class=\"josh-faq__answer\"\u003e\n\u003cp\u003eIncubate for 2 minutes at room temperature protected from light. The signal remains stable for up to 3 hours when protected from light.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\n\u003csummary class=\"josh-faq__question\"\u003e What catalog numbers and kit sizes are available? \u003cspan class=\"josh-faq__icon\"\u003e\u003c\/span\u003e \u003c\/summary\u003e\n\u003cdiv class=\"josh-faq__answer\"\u003e\n\u003cp\u003eFS-T1005-S is listed as 100 reactions, and FS-T1005 is listed as 1,200 reactions.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\n\u003csummary class=\"josh-faq__question\"\u003e How should the kit be shipped and stored? \u003cspan class=\"josh-faq__icon\"\u003e\u003c\/span\u003e \u003c\/summary\u003e\n\u003cdiv class=\"josh-faq__answer\"\u003e\n\u003cp\u003eShip on ice. Store all components at 2–8°C protected from light.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003c\/div\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca rel=\"noopener\" type=\"application\/pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1x_dsDNA_HS_Assay_Kit-User_guide.pdf?v=1789625733\" target=\"_blank\"\u003e\u003cstrong\u003eProduct User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Qihengxing","offers":[{"title":"100 Reactions","offer_id":53192830124213,"sku":"FS-T1005-S","price":29.0,"currency_code":"USD","in_stock":true},{"title":"1200 Reactions","offer_id":53192830156981,"sku":"FS-T1005","price":299.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/dsDNA_HS_Assay_Kit_white_1500.jpg?v=1789626531","url":"https:\/\/biofargo.com\/products\/1x-dsdna-hs-assay-kit","provider":"Biofargo","version":"1.0","type":"link"}