{"product_id":"1x-dsdna-hs-assay-kit-starlighter","title":"1X dsDNA HS Assay Kit——StarLighter","description":"\u003csection\u003e\n  \u003ch2\u003eDescription\u003c\/h2\u003e\n\n  \u003cp\u003eLaboratories quantifying low-concentration double-stranded DNA may encounter measurement interference when using traditional UV absorbance at 260 nm. RNA, proteins, salts, free nucleotides, solvents, and detergents can affect DNA measurements, while preparing a working solution before every assay adds another handling step.\u003c\/p\u003e\n\n  \u003cp\u003eThe StarLighter 1× dsDNA HS Assay Kit provides a ready-to-use fluorescence-based method for high-sensitivity double-stranded DNA quantification. The supplied 1× dsDNA HS Working Solution does not require dilution or preparation before use. Add the required volume of sample to the working solution, incubate for 2 minutes at room temperature while protected from light, and measure the fluorescence.\u003c\/p\u003e\n\n  \u003cp\u003eThis ready-to-use 1X dsDNA HS assay kit is highly selective for dsDNA over RNA. Depending on sample volume, it supports initial dsDNA concentrations from 10 pg\/µL to 100 ng\/µL and a total assay range of 0.2–100 ng. Each assay uses 1–20 µL of sample in a final volume of 200 µL.\u003c\/p\u003e\n\n  \u003cp\u003eThe fluorescent dsDNA quantitation kit is compatible with Qubit 2, Qubit 3, Qubit 4, and Qubit Flex fluorometers. It can also be used with a fluorescence microplate reader at 485 nm excitation and 530 nm emission. When protected from light, the fluorescent signal remains stable for up to 3 hours after incubation.\u003c\/p\u003e\n\n  \u003cp\u003e\u003cstrong\u003eFor Research Use Only. Not for use in diagnostic or therapeutic procedures.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/section\u003e\n\n\u003csection\u003e\n  \u003ch2\u003eSpecification\u003c\/h2\u003e\n\n  \u003cdiv style=\"width:100%; overflow-x:auto; -webkit-overflow-scrolling:touch;\"\u003e\n    \u003ctable style=\"width:100%; min-width:680px; border-collapse:collapse;\"\u003e\n      \u003ctbody\u003e\n        \u003ctr\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eProduct Name\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eStarLighter 1× dsDNA HS Assay Kit\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eAssay Type\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eHigh Sensitivity dsDNA Assay\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eTarget\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eDouble-stranded DNA (dsDNA)\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eDetection Method\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eFluorescence\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eFormulation\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eReady-to-use 1× dsDNA HS Working Solution\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eInitial Sample Concentration\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e10 pg\/µL–100 ng\/µL, depending on sample volume\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eTotal Assay Range\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e0.2–100 ng\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eSample Volume\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e1–20 µL\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eFinal Assay Volume\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e200 µL per tube or well\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eAssay Temperature\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eRoom temperature, 20–25°C\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eIncubation\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e2 minutes at room temperature, protected from light\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eSignal Stability\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eUp to 3 hours after incubation when protected from light\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eCompatible Fluorometers\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eQubit 2, Qubit 3, Qubit 4, and Qubit Flex fluorometers\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eMicroplate Reader Compatibility\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eFluorescence microplate reader with 485 nm excitation and 530 nm emission\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eRecommended Microplate\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eBlack-wall, clear-bottom 96-well microplate\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eShipping Condition\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eShip on ice\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eStorage\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e2–8°C, protected from light\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eIntended Use\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eResearch Use Only\u003c\/td\u003e\n        \u003c\/tr\u003e\n      \u003c\/tbody\u003e\n    \u003c\/table\u003e\n  \u003c\/div\u003e\n\n  \u003ch3\u003eAvailable Sizes and Kit Components\u003c\/h3\u003e\n\n  \u003cdiv style=\"width:100%; overflow-x:auto; -webkit-overflow-scrolling:touch;\"\u003e\n    \u003ctable style=\"width:100%; min-width:760px; border-collapse:collapse;\"\u003e\n      \u003cthead\u003e\n        \u003ctr\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eComponent\u003c\/th\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eFS-T1005-S — 100 Reactions\u003c\/th\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eFS-T1005 — 1,200 Reactions\u003c\/th\u003e\n        \u003c\/tr\u003e\n      \u003c\/thead\u003e\n      \u003ctbody\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e1× dsDNA HS Working Solution\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e20 mL\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e250 mL\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003edsDNA HS Standard QS0, 0 ng\/µL\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e100 µL\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e5 mL\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003edsDNA HS Standard QS1, 10 ng\/µL\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e100 µL\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e5 mL\u003c\/td\u003e\n        \u003c\/tr\u003e\n      \u003c\/tbody\u003e\n    \u003c\/table\u003e\n  \u003c\/div\u003e\n\u003c\/section\u003e\n\n\u003csection\u003e\n  \u003ch2\u003eFeatures\u003c\/h2\u003e\n\n  \u003cul\u003e\n    \u003cli\u003e\n\u003cstrong\u003eReady-to-use formulation:\u003c\/strong\u003e The supplied 1× dsDNA HS Working Solution can be used directly without preparing a diluted working solution.\u003c\/li\u003e\n    \u003cli\u003e\n\u003cstrong\u003eHigh-sensitivity dsDNA quantification:\u003c\/strong\u003e The assay covers a total DNA range of 0.2–100 ng.\u003c\/li\u003e\n    \u003cli\u003e\n\u003cstrong\u003eSelective dsDNA measurement:\u003c\/strong\u003e The assay is highly selective for double-stranded DNA over RNA.\u003c\/li\u003e\n    \u003cli\u003e\n\u003cstrong\u003eFlexible sample input:\u003c\/strong\u003e Each assay accepts 1–20 µL of sample in a final volume of 200 µL.\u003c\/li\u003e\n    \u003cli\u003e\n\u003cstrong\u003eShort incubation:\u003c\/strong\u003e Fluorescent signal development requires 2 minutes at room temperature while protected from light.\u003c\/li\u003e\n    \u003cli\u003e\n\u003cstrong\u003eStable reading window:\u003c\/strong\u003e The signal remains stable for up to 3 hours when samples are protected from light.\u003c\/li\u003e\n    \u003cli\u003e\n\u003cstrong\u003eMultiple instrument formats:\u003c\/strong\u003e The kit can be used with Qubit fluorometers or a fluorescence microplate reader.\u003c\/li\u003e\n    \u003cli\u003e\n\u003cstrong\u003eContaminant tolerance:\u003c\/strong\u003e The assay was evaluated in the presence of salts, solvents, detergents, protein, ssDNA, RNA, PEG, and dNTPs.\u003c\/li\u003e\n  \u003c\/ul\u003e\n\n  \u003ch3\u003eContaminant Tolerance\u003c\/h3\u003e\n\n  \u003cp\u003eDNA standards were tested in the presence or absence of the contaminants listed below. An “OK” result usually represents less than 10% perturbation at the indicated final assay concentration.\u003c\/p\u003e\n\n  \u003cdiv style=\"width:100%; overflow-x:auto; -webkit-overflow-scrolling:touch;\"\u003e\n    \u003ctable style=\"width:100%; min-width:760px; border-collapse:collapse;\"\u003e\n      \u003cthead\u003e\n        \u003ctr\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eContaminant\u003c\/th\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eFinal Concentration in Assay\u003c\/th\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eApproximate Concentration in 10 µL Sample\u003c\/th\u003e\n          \u003cth style=\"text-align:left; padding:10px; border:1px solid #ddd;\"\u003eResult\u003c\/th\u003e\n        \u003c\/tr\u003e\n      \u003c\/thead\u003e\n      \u003ctbody\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eBSA\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e10 mg\/mL\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e200 mg\/mL\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eOK\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eSodium Acetate\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e20 mM\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e400 mM\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eOK\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eAmmonium Acetate\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e20 mM\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e400 mM\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eOK\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eSodium Chloride\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e20 mM\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e400 mM\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eOK\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eMagnesium Chloride\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e5 mM\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e100 mM\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eOK\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003ePhenol\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e0.1%\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e2%\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eOK\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eChloroform\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e0.5%\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e10%\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eOK\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eEthanol\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e0.5%\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e10%\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eOK\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eSDS\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e0.01%\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e0.2%\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eOK\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eTriton X-100\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e0.01%\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e0.2%\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eOK\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003essDNA\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e1×\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e1×\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eOK\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003ePolyethylene Glycol (PEG)\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e1%\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e20%\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eOK\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eRNA\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e1×\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e1×\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eOK\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003edNTPs\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e100 µM\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003e2 mM\u003c\/td\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #ddd;\"\u003eOK\u003c\/td\u003e\n        \u003c\/tr\u003e\n      \u003c\/tbody\u003e\n    \u003c\/table\u003e\n  \u003c\/div\u003e\n\u003c\/section\u003e\n\n\u003csection\u003e\n  \u003ch2\u003eApplication\u003c\/h2\u003e\n\n  \u003ch3\u003eHigh-Sensitivity dsDNA Quantification with a Qubit Fluorometer\u003c\/h3\u003e\n\n  \u003col\u003e\n    \u003cli\u003eEquilibrate all kit components to room temperature before use.\u003c\/li\u003e\n    \u003cli\u003ePrepare two standard tubes and the required number of sample tubes.\u003c\/li\u003e\n    \u003cli\u003eUse only thin-wall, clear 0.5 mL PCR tubes.\u003c\/li\u003e\n    \u003cli\u003eLabel the tube lids. Do not label the sides of the tubes because this may interfere with sample reading.\u003c\/li\u003e\n    \u003cli\u003eAdd 190 µL of ready-to-use 1× dsDNA HS Working Solution to each standard tube.\u003c\/li\u003e\n    \u003cli\u003eAdd 180–199 µL of Working Solution to each sample tube, depending on the sample volume.\u003c\/li\u003e\n    \u003cli\u003eAdd 10 µL of Standard QS0 to the first standard tube and 10 µL of Standard QS1 to the second standard tube.\u003c\/li\u003e\n    \u003cli\u003eAdd 1–20 µL of sample to the appropriate sample tube. The final volume must be 200 µL.\u003c\/li\u003e\n    \u003cli\u003eWhen adding 1–2 µL of sample, use a 2 µL pipette for best results.\u003c\/li\u003e\n    \u003cli\u003eVortex each tube vigorously for 3–5 seconds.\u003c\/li\u003e\n    \u003cli\u003eIncubate all tubes for 2 minutes at room temperature while protected from light.\u003c\/li\u003e\n    \u003cli\u003eOn the Qubit 4 Fluorometer, select \u003cstrong\u003edsDNA\u003c\/strong\u003e and then \u003cstrong\u003e1× dsDNA High Sensitivity\u003c\/strong\u003e.\u003c\/li\u003e\n    \u003cli\u003eRead Standard QS0 followed by Standard QS1, then proceed to the samples.\u003c\/li\u003e\n    \u003cli\u003eSelect the sample volume and output concentration units before reading each sample.\u003c\/li\u003e\n  \u003c\/ol\u003e\n\n  \u003cp\u003eDo not hold assay tubes in your hand before reading because warming the solution may change the fluorescence measurement. Do not use calibration curves generated with other kits.\u003c\/p\u003e\n\n  \u003ch3\u003eHigh-Sensitivity dsDNA Quantification with a Fluorescence Microplate Reader\u003c\/h3\u003e\n\n  \u003col\u003e\n    \u003cli\u003eEquilibrate all kit components to room temperature and mix thoroughly by vortexing.\u003c\/li\u003e\n    \u003cli\u003eUse a black-wall, clear-bottom 96-well microplate to minimize cross-talk.\u003c\/li\u003e\n    \u003cli\u003eAdd 190 µL of ready-to-use 1× dsDNA HS Working Solution to each standard well.\u003c\/li\u003e\n    \u003cli\u003eAdd 180–199 µL of Working Solution to each sample well, depending on the sample volume.\u003c\/li\u003e\n    \u003cli\u003eSerially dilute Standard QS1 to prepare standards ranging from 0 to 10 ng\/µL.\u003c\/li\u003e\n    \u003cli\u003eAdd 10 µL of each standard to the designated standard wells.\u003c\/li\u003e\n    \u003cli\u003eAdd 1–20 µL of each sample to the corresponding sample wells.\u003c\/li\u003e\n    \u003cli\u003eEnsure that the final volume in every well is exactly 200 µL.\u003c\/li\u003e\n    \u003cli\u003eVortex the plate briefly for 10–15 seconds.\u003c\/li\u003e\n    \u003cli\u003eIncubate the microplate for 2 minutes at room temperature while protected from light.\u003c\/li\u003e\n    \u003cli\u003eSet the fluorescence microplate reader to 485 nm excitation and 530 nm emission.\u003c\/li\u003e\n    \u003cli\u003eRead the fluorescence of the standards and samples.\u003c\/li\u003e\n    \u003cli\u003eGenerate a standard curve from the serially diluted standards and calculate the concentrations of the unknown samples.\u003c\/li\u003e\n  \u003c\/ol\u003e\n\n  \u003cp\u003eIf a sample concentration exceeds the linear range, dilute the sample appropriately and repeat the assay.\u003c\/p\u003e\n\u003c\/section\u003e\n\n\u003csection\u003e\n  \u003ch2\u003eFAQ\u003c\/h2\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhat is the StarLighter 1× dsDNA HS Assay Kit?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eIt is a ready-to-use high-sensitivity fluorescence assay for quantifying double-stranded DNA. The kit includes a premixed 1× dsDNA HS Working Solution and two dsDNA standards.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eDoes the 1× dsDNA HS Working Solution require dilution?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eNo. The supplied 1× dsDNA HS Working Solution is ready to use and does not require preparation or dilution before adding standards or samples.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhat is the assay range of the ready-to-use dsDNA assay kit?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eThe total assay range is 0.2–100 ng. Depending on sample volume, the supported initial dsDNA concentration range is 10 pg\/µL–100 ng\/µL.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eIs this 1X dsDNA HS assay kit compatible with Qubit fluorometers?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eYes. The protocol supports Qubit 2, Qubit 3, Qubit 4, and Qubit Flex fluorometers. On a Qubit 4 Fluorometer, select dsDNA and then 1× dsDNA High Sensitivity.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eCan the kit be used with a fluorescence microplate reader?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eYes. Use a black-wall, clear-bottom 96-well microplate and set the fluorescence reader to 485 nm excitation and 530 nm emission.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eHow much sample is required for each dsDNA assay?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eEach assay requires 1–20 µL of sample in a final volume of 200 µL. When adding only 1–2 µL of sample, use a 2 µL pipette for best results.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eHow long should the assay be incubated?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eIncubate the tubes or microplate for 2 minutes at room temperature while protected from light. The signal remains stable for up to 3 hours when protected from light.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhat catalog numbers and kit sizes are available?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eFS-T1005-S contains reagents for 100 reactions. FS-T1005 contains reagents for 1,200 reactions according to the supplied product manual.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eHow should the kit be shipped and stored?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eShip the kit on ice. Store all components at 2–8°C and protect them from light.\u003c\/p\u003e\n  \u003c\/details\u003e\n\u003c\/section\u003e","brand":"Qihengxing","offers":[{"title":"100 Reactions","offer_id":53192830124213,"sku":"FS-T1005-S","price":0.0,"currency_code":"USD","in_stock":false},{"title":"1200 Reactions","offer_id":53192830156981,"sku":"FS-T1005","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/StarLighter_1X_dsDNA_HS_Assay_Kit.jpg?v=1788226288","url":"https:\/\/biofargo.com\/products\/1x-dsdna-hs-assay-kit-starlighter","provider":"Biofargo","version":"1.0","type":"link"}